Standard Reference Materials Derived From Ginkgo biloba

Samuel B. Howerton, Catherine A. Rimmer, Lane. C. Sander, Stephen A. Wise

 

 

Ginkgo biloba, a tree of the Ginkgophyta division, has been documented in Traditional Chinese Medicine for the treatment of pneumonic tuberculosis and asthma, as well as poor circulation.  In the past century, extracts of Ginkgo biloba leaves have been used to treat ailments associated with Alzheimer’s disease and other disorders linked to poor cerebral blood circulation.  As a result of these medicinal effects, the worldwide sale of Ginkgo biloba dietary supplements has been estimated at $1 billion (U.S.).

 

As part of an ongoing investigation of botanical supplements, three new Standard Reference Materials (SRM) are under development based on natural, extracted, and processed Ginkgo biloba materials.  These materials are intended primarily for use in validating analytical methods for the determination of ginkgolides and flavanoid aglycones.  These materials may also be used as control samples to support the analysis of Ginkgo biloba dietary supplements.  Levels of the potentially active constituents (ginkgolides and flavanoid aglycones) will be determined in the SRMs.

 

Traditionally, the certification of SRMs has involved the use of two independent analytical techniques for the determination of the constituent compounds.  For the analysis of Ginkgo biloba, liquid chromatography coupled with ultraviolet spectrophotometric detection (LC-UV), as well as mass spectrometric detection (LC-MS), have been chosen as the principal techniques for the determination of the analytes.  In addition to the use of different analytical techniques, studies of the extraction efficiency, and the hydrolysis of the glycosides to the aglycones, have been carried out.

 

 

Name:  Samuel B. Howerton

Advisor:  Lane C. Sander

Division:  Analytical Chemistry

Laboratory:  Chemical Science and Technology

Address:  Build 227, B132,  MS 8392

Phone:  301-975-4756

Fax:  301-977-0685

e-mail:  samuel.howerton@nist.gov

Section: Chemistry