Determination of Troponin Subunits in Human Plasma Using Capillary Electrophoresis*

Dalluge, J.J.*; Bunk, D.M.; Davidson, Y.Y and Welch, M.J.

Analytical Chemistry Division, Chemical Science and Technology Laboratory, 222/B208
National Institute of Standards and Technology, Gaithersburg, MD 20899

Recently, the measurement of cardiac troponins I (cTnI) and T (cTnT) has become the preferred biochemical test for the diagnosis of myocardial infarction because cTnI and cTnT are believed to represent more sensitive and tissue-specific markers than the more conventional markers creatine kinase and lactate dehydrogenase. A limitation of currently used immunoassays, however, is that troponin concentration values can differ by as much as an order of magnitude between different immunoassay kits. This discrepancy necessitates the development of accurate reference methods for the measurement of these troponins in plasma, making possible the preparation of cTnI and cTnT standards. This report describes initial efforts toward the use of capillary electrophoresis (CE) for the separation and detection of troponin subunits in human plasma. Conditions for the determination of cTnI, and cTnT using CE, as well as the potential of pre-concentration techniques for the selective analysis of troponins in complex matrices are described.

* This abstract has not been reviewed by WERB