Dalluge, J.J.*; Bunk, D.M.; Davidson, Y.Y and Welch, M.J.
Recently, the measurement of cardiac troponins I (cTnI) and T (cTnT)
has become the preferred biochemical test for the diagnosis of myocardial
infarction because cTnI and cTnT are believed to represent more sensitive
and tissue-specific markers than the more conventional markers creatine
kinase and lactate dehydrogenase. A limitation of currently used immunoassays,
however, is that troponin concentration values can differ by as much as
an order of magnitude between different immunoassay kits. This discrepancy
necessitates the development of accurate reference methods for the measurement
of these troponins in plasma, making possible the preparation of cTnI and
cTnT standards. This report describes initial efforts toward the use of
capillary electrophoresis (CE) for the separation and detection of troponin
subunits in human plasma. Conditions for the determination of cTnI, and
cTnT using CE, as well as the potential of pre-concentration techniques
for the selective analysis of troponins in complex matrices are described.
* This abstract has not been reviewed by WERB