Biophysical properties of the Bacillus anthracis PA63 ion channel

 

B. Nablo1, K.M. Halverson2, R.G. Panchal2, S. Bavari2, T.L. Nguyen3,

R. Gussio3, O. Krasilnikov4, M. Misakian1 and J.J. Kasianowicz1

1 NIST, 2USAMRIID, 3NCI-SAIC, 4 U. Pernambuco

 

Bacterial pore-forming toxins (e.g., Staphylococcus aureus alpha-hemolysin) have the potential to selectively detect ions and distinguish between different types of single-stranded DNA. More recently, based on its ability to strongly bind full-length Lethal Factor secreted by Bacillus anthracis, the channel formed by the activated version of B. anthracis protective antigen (i.e., PA63) was used to monitor the effectiveness of a therapeutic agent against anthrax infection. To better adapt the PA63 channel for high-throughput screening and clinical applications, we are biophysically characterizing the PA63 channel with several different classes of polymers.

 

 

Author Information:

 

Author - Brian Nablo

Mentor Michael Gaitan and John Kasianowicz

Division 812.05 EEEL Semiconductor Electronics

Laboratory Enabling Devices

Address 225/A308

Mail Stop 812.05

Phone x2492

FAX 301-948-4081

Email brian.nablo@nist.gov

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