Biophysical properties of the Bacillus anthracis PA63 ion channel


B. Nablo1, K.M. Halverson2, R.G. Panchal2, S. Bavari2, T.L. Nguyen3,

R. Gussio3, O. Krasilnikov4, M. Misakian1 and J.J. Kasianowicz1

1 NIST, 2USAMRIID, 3NCI-SAIC, 4 U. Pernambuco


Bacterial pore-forming toxins (e.g., Staphylococcus aureus alpha-hemolysin) have the potential to selectively detect ions and distinguish between different types of single-stranded DNA. More recently, based on its ability to strongly bind full-length Lethal Factor secreted by Bacillus anthracis, the channel formed by the activated version of B. anthracis protective antigen (i.e., PA63) was used to monitor the effectiveness of a therapeutic agent against anthrax infection. To better adapt the PA63 channel for high-throughput screening and clinical applications, we are biophysically characterizing the PA63 channel with several different classes of polymers.



Author Information:


Author - Brian Nablo

Mentor Michael Gaitan and John Kasianowicz

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Laboratory Enabling Devices

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