Quantifying Cell Migration on Covalent Peptide Gradient Surfaces

Matt Kipper

Member, Iowa State University Chapter of Sigma Xi

Advisor: Francis Wang

 

Polymers Division

Materials Science and Engineering Laboratory

Bldg. 224, Rm. A111, MS 8543

Phone: 5439

Fax: 9777

mkipper@nist.gov

 

Biotechnology

The migration of cells that are involved in the early (inflammation) stage of wound healing, such as neutrophils, macrophages, and T lymphocytes has been extensively studied for several decades. However, the migration of slower migrating cells, such as fibroblasts and endothelial cells, which are involved in the later stages† of wound healing (proliferation and remodeling), is more difficult to study. Recent research in fibroblast migration has contributed to our understanding of the cellular mechanisms of migration as well as useful models of the dynamics of migrating cell populations. What we need now are models that can bridge the gap between the cellular mechanisms of migration and the biological response measured from a population of cells in a given experiment. Such models are essential for the translation of experimental results to design of novel materials for practical tissue engineering applications.

 

Modeling cell migration can be difficult because there is significant variance within a population of cells. In this poster Iíll demonstrate improvements on current models of cell migration that account for various sources of error in the cell migration assays and that can accurately quantify parameters associated with the cell migration.