DETERMINATION OF GINSENOSIDES USING LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

 

 

Christian M. White and Karen Phinney

 

 

Ginseng has been used in traditional medicine as an adaptogen, antioxidant, and stimulant.  There has also been research into the effectiveness of ginseng as a treatment for a myriad of conditions such as cardiovascular disease, cancer, type 2 diabetes mellitus, and insomnia.  Ginsenosides are a class of steroid glycosides that are thought to be the primary compounds thought to contribute to the efficacy of ginseng.  They are triterpene saponins and are split into two groups based on the presence of a monosubstituted diol or triol core.  The most prevalent ginsenosides found in Panax quinquefolius (American ginseng) are ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1, with ginsenoside Rf being prevalent in Panax ginseng (Asian ginseng) only.  Currently NIST is developing SRMs for both ginseng-derived material and ginsenosides.  This poster details the development of methods based on liquid chromatography-mass spectrometry for the determination of ginsenosides in solution.