STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF RECONSTITUTED G-PROTEIN COUPLED MEMBRANE RECEPTORS BY NEUTRON SCATTERING AND SOLID-STATE NUCLEAR MAGNETIC RESONANCE

Amit Vaish, Vitalii Silin, David Vanderah, Marlon Walker, Klaus Gawrisch, Charles Majkrzak and Susan Krueger

 

 

We are developing methods to fabricate highly conformal high aspect-ratio metal nanostructures using atomic layer deposition on alumina membrane. The surface of these nanotube membranes are further functionalized by self-assembly of thiol-terminated molecules for biosensing applications. In particular, we are interested in fabricating functionalized nanostructure to selectively capture an important class of protein, G-protein coupled receptor (GPCR), to elucidate the structure-function relationship of membrane proteins by solid state NMR and neutron reflectivity. We are also developing two kinds of self-assembly based biomaterials (1) materials with excellent non-fouling properties (2) materials which can provide a “chemical hook” to selectively capture GCPR’s to a surface. Surface coating and functionalization is analyzed by scanning electron microscopy, X‑ray photoelectron spectroscopy, solid-state NMR and ellipsometry.