COMPARING PROTEIN TERTIARY STRUCTURE BY CHARACTERIZING ENSEMBLE DYNAMICS
Napoleon Tercero; Curt Meuse; Joe Hubbard
The biopharmaceutical industry is currently having problems developing measurements capable of showing that the properties of the biomolecules in a specific dose of medicine are the same as the properties of the biomolecules in the drug that were shown to be safe and effective in clinical trials. Generally, a combination of orthogonal measurements, such as mass spectrometry, chromatography, NMR, etc., are used to compare the characteristics of the biopharmaceuticals. The disadvantage of this approach is, that a larger percentage of the tested batches get rejected with each independent technique used resulting in a significant and costly waste of product.
propose to link a pair of infrared spectroscopic measurements, through the
kinetic processes of protein ensemble dynamics, to allow us to assess the
similarity between two