Rachel A. Lieberman and Lane C. Sander


††††††††††† St. Johnís wort (SJW, Hypericum perforatum L.) has been used as a traditional medicine in the treatment of abdominal pain, wound healing burns, and as an antidepressant.† The unique marker compounds of SJW include naphthodianthrones (hypericin and pseudohypericin) and phloroglucinols (hyperforin and adhyperforin).† The levels of these phytochemical components may vary in floral, leaf, and stem tissue.† The National Institute of Standards and Technology (NIST) in collaboration with the National Institutes of Health Office of Dietary Supplements (NIH-ODS) and the Food and Drug Administration (FDA) is producing a suite of SJW Standard Reference Materials (SRMs) including a powdered leaf, methanolic extract, carbon dioxide extract, and finished product forms.† In addition, calibration solutions for hypericin and pseudohypericin are under development as part of the SJW SRM suite.† These SRMs are intended to assist dietary supplement manufacturers in providing accurate values for their product labels.

††††††††††† This poster presents the development of three analytical methods for the determination of hypericin in SRM 3266 Dianthrones Calibration Solution.† Gravimetric values were determined when the calibration solution was prepared and ampouled.† Two independent methods based on liquid chromatography with ultraviolet/fluorescence (LC/UV/FL) and mass spectrometry (LC/MS) detection were developed to measure analytically the amount of hypericin present in SRM 3266.† The details of each method and the assignment of concentration values will be discussed.† Future efforts will evaluate the long term stability of the SRM solution.† Development of a related pseudohypericin calibration solution is also planned.