STABILIZATION OF PROTEINS BY NANOENCAPSULATION IN SUGAR-GLASS FOR TISSUE ENGINEERING AND DRUG DELIVERY APPLICATIONS

Jyotsnendu Giri, Marcus T Cicerone.

 

We demonstrate a method of encapsulating proteins in sugar-glass nanoparticles that provides a generic platform for incorporating and delivering proteins from vehicles such as microspheres or tissue scaffolds for therapeutic or regenerative medicine.  This encapsulation method almost entirely overcomes the well-known activity loss problems associated with polymer and solvent interaction during processing, especially for lower polarity solvents.  It also intrinsically stabilizes the proteins against deactivation during storage of final product.  Furthermore, the nanoparticles facilitate excellent solvent dispersion, resulting in uniform distribution and slow release of biomolecules from the scaffold.  In this work, we first contrast robustness of encapsulated and unencapsulated proteins to conventional processing techniques.  We then demonstrate the effectiveness of our system for room-temperature storage and release kinetics of biomolecules from the polymer scaffolds.   We highlight work using four proteins (Peroxidase from horseradish (Type II), Lipase from Candida rugosa (Type VII) , Recombinant Human BMP-2, Human Insulin) to demonstrate the universality of our approach.