MEASURING NANOPARTICLE DYNAMICS WITH REAL-TIME TRACKING

Kan Du, Philip Campbell, Andrew Berglund

Nanoparticles in liquids are an important platform for nanofabrication and nanomanufacturing processes. Few methods are available for measuring time-resolved dynamics of individual nanoparticles at nanoscale spatial resolution. We have developed an apparatus that enables us to measure the motion and dynamics of individual nanoparticles in liquid environments using real-time, three-dimensional particle-tracking . Real-time feedback control methods enable us to monitor dynamics of individual nanoparticles by locking them in focus in an optical microscope, enhancing both the temporal and spatial resolution of our instrument. These tools can be applied to study nanoparticle binding, self-assembly processes, and single-molecule biophysics.