Method DEVELOPMENT for the determination of Polyphenolic Constituents of Black Cohosh


Rosalind S.C Chia and Lane C. Sander


Black cohosh [Actaea racemosa L. (synonym: Cimicifuga racemosa)] has been used in the treatment of menopausal symptoms. The two principal groups of compounds are triterpenoid glycosides and polyphenolic derivatives. With regards to polyphenolic derivatives, one group of compounds, hydroxycinnamic acid derivatives (caffeic acid, ferulic acid and isoferulic acid) is isolated from the roots and rhizomes of black cohosh. The levels of these phytochemical components may vary in the leaves, roots and rhizomes and different species. The National Institute of Standards and Technology (NIST) in collaboration with the National Institutes of Health Office of Dietary Supplements (NIH-ODS) and the Food and Drug Administration (FDA) is producing a suite of Black Cohosh Standard Reference Materials (SRMs) including a powdered leaf, root and rhizome extract, powdered rhizome and finished product forms.



            This poster presents the development of soxhlet extraction procedure used to determine the presence of hydroxycinnamic acid derivatives in SRM 3295 powdered rhizome and SRM 3297 powdered root and rhizome extract. Additionally two methods, using different solvent systems and columns, were developed to measure analytically the amount of each hydrooxycinnamic acid derivatives present in SRM 3295 and 3297. The details of the sample preparation and analytical method will be discussed. Further studies will include the quantitation of these hydroxycinnamic acids for all the Black Cohosh related SRMs.