COMBINATORIAL AND HIGH-THROUGHPUT SCREENING OF CELL FATES IN 3D TISSUE SCAFFOLDS

Kaushik Chatterjee1,2, Marian F. Young2, Carl G. Simon, Jr.1

1Polymers Division, National Institute of Standards and Technology, Gaithersburg MD

2National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda MD

 

Towards accelerating the pace of tissue engineering research there is a need to develop combinatorial and high-throughput (CHT) methods to screen cell-biomaterial interactions to maximize tissue generation. Previous CHT methods to screen cell response to biomaterial utilized planar 2D culture formats where cells were seeded on material surfaces. Biomaterials must be presented as 3D scaffolds for tissue generation and cells are sensitive to topographical differences between 2D surfaces and 3D scaffolds. Furthermore, cell response in 3D culture is more representative of their in vivo behavior than in 2D formats. Thus, we have developed CHT methods to screen cell response in 3D. Osteoblast responses to systematically varied properties such as scaffold stiffness and scaffold composition have been screened using gradients and arrays. The results demonstrate that combinatorial methods can be applied for screening 3D tissue scaffolds and give insights on scaffold library design as well as design parameters for fabricating scaffolds that maximize tissue regeneration.