MONITORING LOCAL NANOPARTICLE HEATING BY FLUORESCENCE MICROSCOPY

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Matthew L. Clarke and Jeeseong Hwang

Optical Technology Division, NIST Gaithersburg, MD 20899.

 

Generation of heat using optically excited nanoparticles can be beneficial or detrimental depending on the application.† Clinically applicable studies are being pursed in an effort to achieve nanoparticle-induced hyperthermia for the treatment of cancerous tissues using optical radiation.† The characterization of heat production on both cellular and tissue levels is important for determination of nanoparticle dosage and optimal conditions for the radiation.† Here, we present a method to monitor the local temperature in in vitro environment on the micrometer scale using a temperature-sensitive fluorescent dye. †This method allows for the spatial and temporally resolved thermal imaging with optical microscopy.† The model system we employ is based on gold nanoshells, which can be photothermally excited by near infrared light.† Multimodal imaging methods allow profiling of the excitation beam and the transfer of heat from the nanoparticles to their surroundings.† We observed that heating and cooling (upon termination of irradiation) occur on the timescale of seconds.† Additionally, local heating induced directional flow of nanoparticles to the site of irradiation. †This increase in the concentration of nanoparticles at the site of photothermal therapy could reduce the number of nanoparticles required for treatment.

 

 

 

 

 

 

 

 


 

CATEGORY: Physics

 

 

Mentors Name: Jeeseong Hwang

Optical Technology, Physics

216/B221, 8443

Tel: 301-975-4580

Fax: 301-975-6991

Email: jeeseong.hwang@nist.gov

 

Is your mentor a Sigma Xi Member?†††† No

 

Authorís name:† Matthew L. Clarke

Optical Technology, Physics

216/B205, 8443

Tel: 301-975-6304

Fax: 301-975-6991

Email: matthew.clarke@nist.gov

 

 

I am not a Sigma Xi member