DEVELOPMENT OF A MICROWAVE-ASSISTED METHOD FOR EXTRACTION OF HYROXYLATED COMPOUNDS FROM HUMAN SERUM

 

Rebecca M. Heltsley1, John Kucklick1, Michele Schantz2

1National Institute of Standards and Technology, Hollings Marine Laboratory, 331 Fort Johnson Rd., Charleston, SC, USA.  2National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg, MD, USA

Recently, endocrine-disrupting compounds, such as phenolic halogenated compounds and hydroxylated PCBs, have gained widespread interest due to their potential health effects in humans and wildlife.  The two primary matrices used to assess human exposure to environmental contaminants are urine and blood.  Blood serves as a circulating reservoir for phenolic and hyroxylated compounds due to their capacity to bind to proteins.  Simple, efficient extraction and analytical techniques for these compounds in blood are a priority.  This study will provide an alternative method for extracting phenolic and neutral compounds from blood using open-focused microwave technology.  The application of a semi-automated microwave extraction technique reduces the time required for sample preparation and provides a relatively high throughput method.  Method development was performed on two candidate reference materials, SRM 1957 Organic Contaminants in Human Serum (non-fortified) and SRM 1958 Organic Contaminants in Human Serum (fortified).  Values generated and certified in these materials will ultimately provide researchers with reference materials to use when extracting hydroxylated analytes in blood. 

 

 

Contact Information:                         Mentor: Michele Schantz

Name: Rebecca M. Heltsley                             NIST-CSTL 839.02

NIST-CSTL-839.02                                        100 Bureau Dr. Mail Stop 8392

Hollings Marine Laboratory                              Gaithersburg , MD 20899-8392

331 Fort Johnson Rd.                                       Office:  301-975-3106

Charleston, SC 29412                                      Fax:  301-977-0685

Office:  843-762-8993                                     Michele.schantz@nist.gov

Fax:  843-762-8742

Rebecca.heltsley@nist.gov

I am not a member of Sigma Xi

Category: Chemistry