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Displaying records 31 to 40 of 273 records.
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31. Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakaraensis DNA primase
Topic: Life Sciences Research
Published: 5/11/2012
Authors: Zvi Kelman, John P Marino, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan, Gary Giulian, Wei Yuan, Shuwei Li
Abstract: In the presence of dATP, glycerol and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis (Tk) catalyzed the formation of two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the Tk p41 cat ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910427

32. Challenges in Microbial Sampling in the Indoor Environment
Series: Technical Note (NIST TN)
Report Number: 1737
Topic: Life Sciences Research
Published: 3/1/2012
Authors: Jayne B Morrow, Autumn S. Downey, Jordan Peccia
Abstract: This report summarizes the results of the Challenges in Microbial Sampling in the Indoor Environment Workshop held on February 14-15, 2011 at the National Institute of Standards and Technology in Gaithersburg, Maryland.
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910577

33. Mathematical Model Formulation and Validation of Water and Cryoprotective Agent Transport in Whole Hamster Pancreatic Islets
Topic: Life Sciences Research
Published: 10/11/2011
Authors: James D. Benson, Charles T Benson, John K Critser
Abstract: A mathematical and conceptual model of water and solute transport for whole hamster pancreatic islets has been developed and experimentally validated incorporating fundamental biophysical data from previous studies on individual hamster islet cells ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906347

34. Segmentation and Cell Tracking of Breast Cancer Cells
Topic: Life Sciences Research
Published: 9/26/2011
Authors: Adele P Peskin, Daniel J. Hoeppner, Christina H. Stuelten
Abstract: We describe a new technique to segment and track the cell images of a breast cancer cell line in order to study cell migration and metastasis. Within each image cell phenotypes vary widely, ranging from very bright completely bounded cells to barely ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908649

35. Thermococcus kodakarensis has two functional PCNA homologues but only one is essential for viability
Topic: Life Sciences Research
Published: 8/5/2011
Author: Zvi Kelman
Abstract: Proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911331

36. Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture
Topic: Life Sciences Research
Published: 5/2/2011
Authors: Kaushik Chatterjee, Marian F Young, Carl George Simon Jr.
Abstract: Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903763

37. A General Model for the Dynamics of Cell Volume, Global Stability, and Optimal Control
Topic: Life Sciences Research
Published: 2/11/2011
Author: James D. Benson
Abstract: Cell volume and concentration regulation in the presence of changing extracellular environments has been studied for centuries, and recently a general nondimensional model was introduced that encompassed solute and solvent transmembrane flux for a wi ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904647

38. Loss of 45 Da from a2 Ions and Preferential Loss of 48 Da from a2 Ions Containing Methionine in Peptide Ion Tandem Mass Spectra
Topic: Life Sciences Research
Published: 1/29/2011
Authors: Yamil Simon, Pedatsur Neta, Xiaoyu Yang, Stephen E Stein
Abstract: While analyzing tandem mass spectra of tryptic tripeptides, intense unassigned peaks were observed, which correspond to a neutral loss of 45 Da from a^d2^ ions. From exact mass analysis this loss is ascribed to formamide (or NH^d3^+CO). Elimination o ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906270

39. Detection of Heteroplasmic Mitochondrial DNA in Single Mitochondria
Topic: Life Sciences Research
Published: 12/17/2010
Authors: Joseph E. (Joseph E.) Reiner, Rani Kishore, Barbara C. Levin, Thomas Albanetti, Nicholas Boire, Ashley Knipe, Kristian Helmerson, Koren Deckman
Abstract: Background Mitochondrial DNA (mtDNA) genome mutations can lead to energy and respiratory-related disorders like myoclonic epilepsy with ragged red fiber disease (MERRF), mitochondrial myopathy, encephalopathy, lactic acidosis and stroke (MELAS) synd ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904019

40. Predicting Segmentation Accuracy for Biological Cell Images
Topic: Life Sciences Research
Published: 12/15/2010
Authors: Adele P Peskin, Alden A Dima, Joe Chalfoun, John T Elliott
Abstract: We have performed segmentation procedures on a large number of images from two mammalian cell lines that were seeded at low density, in order to study trends in the segmentation results and make predictions about cellular features that affect segm ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906721



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