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Displaying records 31 to 40 of 151 records.
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31. Melting Point Equations for the Ternary System Water/Sodium Chloride/Ethylene Glycol Revisited
Topic: Life Sciences Research
Published: 12/1/2010
Authors: James D. Benson, Aniruddha Bagchi, Xu Han, John K Critser, Erik J Woods
Abstract: Partial phase diagrams are of considerable utility in the development of optimized cryobiological procedures. Recent theoretical predictions of the melting points of ternary solutions of interest to cryobiology have caused us to re-examine measurment ...

32. The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo
Topic: Life Sciences Research
Published: 3/16/2010
Authors: Minin Liu, Viswanath Bandaru, Jeffrey Bond, Pawel Jaruga, Xiaobei Zhao, Plamen P Christov, Cynthia Burrows, Carmelo J. Rizzo, M Miral Dizdar, Susan Wallace
Abstract: To protect cells from oxidative DNA damage and mutagenesis, organisms possess multiple glycosylases to recognize the damaged bases and to initiate the Base Excision Repair (BER) pathway. Recently, three DNA glycosylases were identified in mammals tha ...

33. Evidence for the involvement of DNA repair enzyme NEIL1 in nucleotide excision repair of (5'R)- and (5'S)-8,5'-Cyclo-2'-deoxyadenosines
Topic: Life Sciences Research
Published: 2/16/2010
Authors: Pawel Jaruga, Yan Xiao, Vladimir Vartanian, R Stephen Lloyd, M Miral Dizdar
Abstract: The DNA repair enzyme NEIL1 is a DNA glycosylase that is involved in the first step of base excision repair (BER) of oxidatively induced DNA damage. NEIL1 exhibits a strong preference for excision of 4,6-diamino-5-formamidopyrimidine (FapyAde) and 2, ...

34. The oxidative DNA glycosylases of Mycobacterium tuberculosis exhibit different substrate specificities from their Escherichia coli counterparts
Topic: Life Sciences Research
Published: 2/4/2010
Authors: Yin Guo, Viswanath Bandaru, Pawel Jaruga, Xiaobei Zhao, Cynthia Burrows, Shigenori Iwai, M Miral Dizdar, Jeffrey Bond, Susan Wallace
Abstract: The DNA glycosylases function in the first step of the base excision repair process that is responsible for removing endogenous oxidative purine and pyrimidine damages from DNA. The DNA glycosylases that remove oxidized DNA bases fall into two genera ...

35. In-plane Homogeneity and Lipid Dynamics in tethered Bilayer Lipid Membranes (tBLMs)
Topic: Life Sciences Research
Published: 1/1/2010
Authors: Siddharth Shenoy, Radu Moldovan, James Fitzpatrick, David J. Vanderah, Markus Deserno, Mathias Losche
Abstract: Tethered bilayer lipid membranes (tBLMs) were prepared by the self-assembly of thiolated lipidic anchors on gold films sputtered on Si wafers, followed by phospholipid precipitation via rapid solvent exchange. They were characterized in their in-plan ...

36. Quantitative Infrared Spectroscopy of Formalin-Fixed, Paraffin-Embedded Tissue Specimens: Paraffin Wax Removal with Organic Solvents
Report Number: 832407
Topic: Life Sciences Research
Published: 12/1/2009
Authors: Curtis W Meuse, Peter E. Dr. Barker
Abstract: Formalin-fixed, paraffin embedded tissue specimens for histopathology are cut to 3-20 μm thickness to allow transmission of light, and optimize tissue morphology. Tissue is stabilized by formalin fixation followed by impregnation with wax in non ...

37. Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase
Topic: Life Sciences Research
Published: 9/1/2009
Authors: Viktoriya Sidorenko, Arthur P Grollman, Pawel Jaruga, M Miral Dizdar, Dmitry Zharhov
Abstract: Human 8-oxoguanine-DNA-glycosylase (OGG1) efficiently removes mutagenic 8-oxoguanine (8-oxoGua) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua) when paired with cytosine in damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead t ...

38. Combinatorial Screening of Hydrogel Properties for 3-D Cell Culture: Effect of Stiffness on Encapsulated Osteoblasts
Topic: Life Sciences Research
Published: 7/15/2009
Authors: Carl George Simon Jr., Kaushik Chatterjee, Sheng Lin-Gibson, William E Wallace III, Marian F Young
Abstract: A combinatorial method to rapidly screen cell-material interactions in three-dimensional (3-D) culture format was developed for accelerating tissue engineering research. Hydrogels of poly(ethylene glycol) dimethacrylate (PEGDM) with gradients in comp ...

39. Glutathione Depletion by Buthionine Sulfoximine Induces Oxidative Damage to DNA in Organs of Rabbits in vivo
Report Number: 901398
Topic: Life Sciences Research
Published: 6/9/2009
Authors: Goksel Gokce, Gonen Ozsarlak, Gulgun Oktay, Guldal Kirkali, Pawel Jaruga, M Miral Dizdar, Zeliha Kerry
Abstract: Glutathione (GSH) exists in mammalian tissues in vivo at high concentrations and plays an important protective role against oxidatively induced damage to biological molecules including DNA. We investigated oxidatively induced damage to DNA by GSH dep ...

40. Oligo(ethylene oxide) Self-Assembled Monolayers, with Self-limiting Packing Densities for the Inhibition of Nonspecific Protein Adsorption
Topic: Life Sciences Research
Published: 5/5/2009
Authors: David J. Vanderah, Ryan Vierling, Marlon L Walker
Abstract: We have created a molecule that forms self-assembled monolayers (SAMs) on Au possessing the characteristics for inhibition of nonspecific protein adsorption, i.e., uniformly distributed, loosely-packed, conformationally mobile, hydrated ethylene oxid ...

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