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Topic Area: Life Sciences Research
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Displaying records 31 to 40 of 251 records.
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31. Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase
Topic: Life Sciences Research
Published: 9/1/2009
Authors: Viktoriya Sidorenko, Arthur P Grollman, Pawel Jaruga, M Miral Dizdar, Dmitry Zharhov
Abstract: Human 8-oxoguanine-DNA-glycosylase (OGG1) efficiently removes mutagenic 8-oxoguanine (8-oxoGua) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua) when paired with cytosine in damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead t ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=902113

32. Protein crystal engineering of YpAC-IV using a strategy of excess charge reduction
Topic: Life Sciences Research
Published: 8/5/2009
Authors: David Travis Gallagher, N N Smith, Sung Kim, Howard Robinson, Prasad T Reddy
Abstract: The class IV adenylyl cyclase from Yersinia pestis has been engineered to enable crystallization at neutral pH for mechanism studies. The wild-type enzyme crystallized only at pH below 5. Based on the unliganded wild-type structure 2FJT at 1.9 Angs ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830569

33. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Topic: Life Sciences Research
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901347

34. Combinatorial Screening of Hydrogel Properties for 3-D Cell Culture: Effect of Stiffness on Encapsulated Osteoblasts
Topic: Life Sciences Research
Published: 7/15/2009
Authors: Carl George Simon Jr, Kaushik Chatterjee, Sheng Lin-Gibson, William E Wallace III, Marian F Young
Abstract: A combinatorial method to rapidly screen cell-material interactions in three-dimensional (3-D) culture format was developed for accelerating tissue engineering research. Hydrogels of poly(ethylene glycol) dimethacrylate (PEGDM) with gradients in comp ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901798

35. Mechanism of Formation of Vesicle Fused Phospholipid Monolayers on Alkanethiol Self-Assembled Monolayer Supports
Topic: Life Sciences Research
Published: 6/15/2009
Authors: John Taylor Woodward IV, Curtis W Meuse
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830453

36. Glutathione Depletion by Buthionine Sulfoximine Induces Oxidative Damage to DNA in Organs of Rabbits in vivo
Report Number: 901398
Topic: Life Sciences Research
Published: 6/9/2009
Authors: Goksel Gokce, Gonen Ozsarlak, Gulgun Oktay, Guldal Kirkali, Pawel Jaruga, M Miral Dizdar, Zeliha Kerry
Abstract: Glutathione (GSH) exists in mammalian tissues in vivo at high concentrations and plays an important protective role against oxidatively induced damage to biological molecules including DNA. We investigated oxidatively induced damage to DNA by GSH dep ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901398

37. Oligo(ethylene oxide) Self-Assembled Monolayers, with Self-limiting Packing Densities for the Inhibition of Nonspecific Protein Adsorption
Topic: Life Sciences Research
Published: 5/5/2009
Authors: David J Vanderah, Ryan Vierling, Marlon L Walker
Abstract: We have created a molecule that forms self-assembled monolayers (SAMs) on Au possessing the characteristics for inhibition of nonspecific protein adsorption, i.e., uniformly distributed, loosely-packed, conformationally mobile, hydrated ethylene oxid ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=900173

38. Plant and fungal Fpg homologs are formamidopyrimidine DNA glycosylases but not 8-oxoguanine DNA glycosylases
Topic: Life Sciences Research
Published: 5/1/2009
Authors: Scott D Kathe, Ramiro Barrantes-Reynolds, Pawel Jaruga, Michael Newton, Cynthia Burrows, Viswanath Bandaru, M Miral Dizdar, Jeffrey Bond, Susan Wallace
Abstract: Formamidopyrimidine DNA glycosylase (Fpg) and endonuclease VIII (Nei) share an overall common three dimensional structure and primary amino acid sequence in conserved structural motifs but have different substrate specificities, with bacterial Fpg pr ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=900972

39. Comparison of Fluorometric and Spectrophotometric DNA Quantification for Real-time Quantitative PCR of Degraded DNA
Topic: Life Sciences Research
Published: 4/1/2009
Authors: Luke A. Shokere, Marcia J Holden, G. Ronald Jenkins
Abstract: Isogenic NK603 DNA was degraded by sonication or heat and quantified using A260 and a fluorescent dye method. qPCR experiments were conducted by amplifying an SSIIb-3 endogenous control and an NK603 transgene in untreated, sonicated, and heat-treate ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832278

40. Profound asymmetry in the structure of the cAMP-free cAMP receptor protein (CRP) from Mycobacterium tuberculosis
Topic: Life Sciences Research
Published: 3/27/2009
Authors: David Travis Gallagher, N N Smith, Soo-Kyung Kim, Howard Robinson, Prasad T Reddy
Abstract: he bacterial cyclic AMP receptor protein (CRP) has been studied extensively as a model allosterically controlled transcription factor, despite the lack of a crystal structure of its inactive (unliganded) form. We report the crystal structure at 2.0 ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832417



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