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Topic Area: Life Sciences Research
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Displaying records 11 to 20 of 266 records.
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11. Capturing Rare Cells From Blood Using a Packed Bed of Custom-Synthesized Chitosan Microparticles
Topic: Life Sciences Research
Published: 7/1/2013
Authors: Chandamany Arya, Jason G Kralj, Kunqiang Jiang, Matthew S Munson, Thomas P Forbes, Don L. DeVoe, Srinivasa R Raghavan, Samuel P Forry
Abstract: Uniform multifunctional chitosan microparticles were produced in large numbers using a simple and inexpensive microtubing arrangement. The particles were functionalized through both physical encapsulation of carbon black, to attenuate autofluores ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912723

12. Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins
Topic: Life Sciences Research
Published: 4/22/2013
Authors: Antony K. Chen, Frank W DelRio, Alexander W Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L Plant
Abstract: The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910923

13. Effect of Surface Chemistry on Stem Cell Response in 2-D vs. 3-D Cell Culture Niches
Topic: Life Sciences Research
Published: 4/19/2013
Authors: Sumona Sarkar, Carl George Simon Jr, Roberta I Lock, Joy P Dunkers
Abstract: To develop tissue engineered devices for regenerative medicine strategies, key scaffold properties for directing stem cell response must be identified. As focus shifts from 2-D to 3-D culture systems in an attempt to better recapitulate the na ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912888

14. Active Transcriptomic and Proteomic Reprogramming in the C. elegans Nucleotide Excision Repair Mutant xpa-1
Topic: Life Sciences Research
Published: 4/10/2013
Authors: Katarzyna Arczewska, Gisele Tomazella, Jessica Lindvall, Henok Kassahun, Silvia Maglioni, Alessandro Torgovnick, Johan Henriksson, Olli Matilainen, Bryce J. Marquis, Bryant C Nelson, Eshrat Babaie, Carina Holmberg, Thomas Burglin, Natascia Ventura, Bernd Thiede, Hilde Nilsen
Abstract: Transcription-blocking oxidative DNA damage is believed to contribute to aging and to underlie activation of oxidative stress-responses and down-regulation of insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice. Here, we p ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913265

15. The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes
Topic: Life Sciences Research
Published: 2/13/2013
Authors: Zvi Kelman, Kira S Makarova, Eugene V Koonin
Abstract: In eukaryotes, the CMG (Cdc45, MCM, GINS) complex functions as the replicative helicase and plays a key role in DNA replication. Archaeal homologs of the eukaryotic MCM and GINS proteins have been identified but until recently no homolog of the CDC4 ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910056

16. Thermococcus kodakarensis DNA replication
Topic: Life Sciences Research
Published: 2/2/2013
Authors: Zvi Kelman, Lori Kelman, Zhuo Li
Abstract: DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began about twenty years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This h ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912749

17. Next-generation Sequencing: Guidance for the Translation from Research to Clinical Applications
Topic: Life Sciences Research
Published: 11/8/2012
Authors: Justin M Zook, Amy Gargis, Lisa Kalman, Ira Lubin
Abstract: We would like to draw your readers‰ attention to the Next-generation Sequencing: Standardization of Clinical Testing (Nex-StoCT) guidelines, which represent an initial step toward ensuring that the results derived from next-generation sequencing (N ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910560

18. Remote sensing of chiral signatures on Mars
Topic: Life Sciences Research
Published: 11/1/2012
Authors: William Sparks, James H. Hough, Thomas Avery Germer, Frank Robb, Ludmilla Kolokolova
Abstract: We describe circular polarization as a remote sensing diagnostic of chiral signatures which may be applied to Mars. The remarkable phenomenon of homochirality provides a unique biosignature which can be amenable to remote sensing through circular ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910692

19. GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES
Topic: Life Sciences Research
Published: 10/28/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911771

20. Characterization of In Vitro Transcription Amplification Linearity and Variability in the Low Copy Number Regime using ERCC Spike Ins
Topic: Life Sciences Research
Published: 10/20/2012
Authors: Jason G Kralj, Marc L Salit
Abstract: Background Characterization of whole transcriptomes from small RNA samples (less than 1 ng total RNA) has been a major challenge. Typically, samples are extracted and purified, amplified by in vitro transcription (IVT), and then subjected to genome- ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=909936



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