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Displaying records 11 to 20 of 139 records.
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11. GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES
Topic: Life Sciences Research
Published: 10/28/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911771

12. Characterization of In Vitro Transcription Amplification Linearity and Variability in the Low Copy Number Regime using ERCC Spike Ins
Topic: Life Sciences Research
Published: 10/20/2012
Authors: Jason G Kralj, Marc L Salit
Abstract: Background Characterization of whole transcriptomes from small RNA samples (less than 1 ng total RNA) has been a major challenge. Typically, samples are extracted and purified, amplified by in vitro transcription (IVT), and then subjected to genome- ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=909936

13. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Topic: Life Sciences Research
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

14. Fabrication of Microelectrodes on Polyester Membranes for Dielectrophoretic Cell Capture
Topic: Life Sciences Research
Published: 6/18/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: We present a new system for cell capturing on permeable polyester (PET) membranes using dielectrophoretic forces. For the first time gold microelectrodes were fabricated on PET membranes using conventional photolithographic techniques. Their charac ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911140

15. Challenges in Microbial Sampling in the Indoor Environment
Series: Technical Note (NIST TN)
Report Number: 1737
Topic: Life Sciences Research
Published: 3/1/2012
Authors: Jayne B Morrow, Autumn S. Downey, Jordan Peccia
Abstract: This report summarizes the results of the Challenges in Microbial Sampling in the Indoor Environment Workshop held on February 14-15, 2011 at the National Institute of Standards and Technology in Gaithersburg, Maryland.
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910577

16. Mathematical Model Formulation and Validation of Water and Cryoprotective Agent Transport in Whole Hamster Pancreatic Islets
Topic: Life Sciences Research
Published: 10/11/2011
Authors: James D. Benson, Charles T Benson, John K Critser
Abstract: A mathematical and conceptual model of water and solute transport for whole hamster pancreatic islets has been developed and experimentally validated incorporating fundamental biophysical data from previous studies on individual hamster islet cells ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906347

17. Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture
Topic: Life Sciences Research
Published: 5/2/2011
Authors: Kaushik Chatterjee, Marian F Young, Carl George Simon Jr.
Abstract: Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903763

18. A General Model for the Dynamics of Cell Volume, Global Stability, and Optimal Control
Topic: Life Sciences Research
Published: 2/11/2011
Author: James D. Benson
Abstract: Cell volume and concentration regulation in the presence of changing extracellular environments has been studied for centuries, and recently a general nondimensional model was introduced that encompassed solute and solvent transmembrane flux for a wi ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904647

19. Melting Point Equations for the Ternary System Water/Sodium Chloride/Ethylene Glycol Revisited
Topic: Life Sciences Research
Published: 12/1/2010
Authors: James D. Benson, Aniruddha Bagchi, Xu Han, John K Critser, Erik J Woods
Abstract: Partial phase diagrams are of considerable utility in the development of optimized cryobiological procedures. Recent theoretical predictions of the melting points of ternary solutions of interest to cryobiology have caused us to re-examine measurment ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905323

20. The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo
Topic: Life Sciences Research
Published: 3/16/2010
Authors: Minin Liu, Viswanath Bandaru, Jeffrey Bond, Pawel Jaruga, Xiaobei Zhao, Plamen P Christov, Cynthia Burrows, Carmelo J. Rizzo, M Miral Dizdar, Susan Wallace
Abstract: To protect cells from oxidative DNA damage and mutagenesis, organisms possess multiple glycosylases to recognize the damaged bases and to initiate the Base Excision Repair (BER) pathway. Recently, three DNA glycosylases were identified in mammals tha ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903370



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