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Topic Area: Life Sciences Research
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Displaying records 1 to 10 of 251 records.
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1. Total free energy governs cell morphology ‹ A possible solution to obtain intrinsic cell modulus
Topic: Life Sciences Research
Published: 9/26/2013
Authors: Martin Y Chiang, Yanzi Yangben , Nancy J Lin, Li Yang
Abstract: Cells adjust their morphology in response to physical and chemical interactions with their environment. In this report, we deem that the fundamental nature of free energies associated with cell/substrate interactions regulates adherent cell morpholog ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=909671

2. Measurement Uncertainty in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7954
Topic: Life Sciences Research
Published: 8/13/2013
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott, Anne L Plant
Abstract: Cell image segmentation is a part of quantitative studies regarding cell movement and cell behavior, and it plays a critical role in molecular biology and cellular biochemistry. Therefore, it is fundamentally important to evaluate the performance ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914171

3. Capturing Rare Cells From Blood Using a Packed Bed of Custom-Synthesized Chitosan Microparticles
Topic: Life Sciences Research
Published: 7/1/2013
Authors: Chandamany Arya, Jason G Kralj, Kunqiang Jiang, Matthew S Munson, Thomas P Forbes, Don L. DeVoe, Srinivasa R Raghavan, Samuel P Forry
Abstract: Uniform multifunctional chitosan microparticles were produced in large numbers using a simple and inexpensive microtubing arrangement. The particles were functionalized through both physical encapsulation of carbon black, to attenuate autofluores ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912723

4. Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins
Topic: Life Sciences Research
Published: 4/22/2013
Authors: Antony K. Chen, Frank W DelRio, Alexander W Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L Plant
Abstract: The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910923

5. Active Transcriptomic and Proteomic Reprogramming in the C. elegans Nucleotide Excision Repair Mutant xpa-1
Topic: Life Sciences Research
Published: 4/10/2013
Authors: Katarzyna Arczewska, Gisele Tomazella, Jessica Lindvall, Henok Kassahun, Silvia Maglioni, Alessandro Torgovnick, Johan Henriksson, Olli Matilainen, Bryce J. Marquis, Bryant C Nelson, Eshrat Babaie, Carina Holmberg, Thomas Burglin, Natascia Ventura, Bernd Thiede, Hilde Nilsen
Abstract: Transcription-blocking oxidative DNA damage is believed to contribute to aging and to underlie activation of oxidative stress-responses and down-regulation of insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice. Here, we p ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913265

6. Remote sensing of chiral signatures on Mars
Topic: Life Sciences Research
Published: 11/1/2012
Authors: William Sparks, James H. Hough, Thomas Avery Germer, Frank Robb, Ludmilla Kolokolova
Abstract: We describe circular polarization as a remote sensing diagnostic of chiral signatures which may be applied to Mars. The remarkable phenomenon of homochirality provides a unique biosignature which can be amenable to remote sensing through circular ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910692

7. GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES
Topic: Life Sciences Research
Published: 10/28/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911771

8. Characterization of In Vitro Transcription Amplification Linearity and Variability in the Low Copy Number Regime using ERCC Spike Ins
Topic: Life Sciences Research
Published: 10/20/2012
Authors: Jason G Kralj, Marc L Salit
Abstract: Background Characterization of whole transcriptomes from small RNA samples (less than 1 ng total RNA) has been a major challenge. Typically, samples are extracted and purified, amplified by in vitro transcription (IVT), and then subjected to genome- ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=909936

9. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Topic: Life Sciences Research
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

10. Fabrication of Microelectrodes on Polyester Membranes for Dielectrophoretic Cell Capture
Topic: Life Sciences Research
Published: 6/18/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: We present a new system for cell capturing on permeable polyester (PET) membranes using dielectrophoretic forces. For the first time gold microelectrodes were fabricated on PET membranes using conventional photolithographic techniques. Their charac ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911140



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