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You searched on: Topic Area: Life Sciences Research

Displaying records 51 to 60 of 142 records.
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51. Temperature-induced structural changes in putidaredoxin: a circular dichroism and UV-VIS absorption study
Topic: Life Sciences Research
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903187

52. Structure and Reaction in the Active Site of Mammalian Adenylyl Cyclase
Topic: Life Sciences Research
Published: 4/8/2004
Authors: Y. S. Lee, Morris Krauss
Abstract: The reaction path for the catalytic conversion of adenosine triphosphate (ATP) to cyclic adenosine monophosphate (cAMP) by the enzyme mammalian adenylyl cyclase has been calculated theoretically using the Hartree-Fock method. The crystal structure of ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830386

53. Crystal Structure of the Complex Between Thrombin and the Central "E" Region of Fibrin
Topic: Life Sciences Research
Published: 3/2/2004
Authors: I Pechik, J Madrazo, M W Mosesson, I Hernandez, G L. Gilliland, L Medved
Abstract: Non-substrate interactions of thrombin with fibrin play an important role in modulating its procoagulant activity. To establish the structural basis for these interactions, we crystallized PPACK-inhibited thrombin in complex with a fragment, Eht , c ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830416

54. Overexpression and Rapid Purification of Escherichia Coli Formamidopyrimidine-DNA Glycosylase
Topic: Life Sciences Research
Published: 3/1/2004
Authors: Prasad T Reddy, Pawel Jaruga, T R O'Connor, H Rodriguez, M Miral Dizdar
Abstract: Formamidopyrimidine DNA glycosylase (Fpg) is a DNA glycosylase with an associated AP lyase activity. As a DNA repair enzyme, Fpg excises several modified bases from DNA associated with exposure to oxidizing agents such as free radicals. Experiments ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830402

55. Membrane Surface Dynamics of DNA-Threaded Nanopores Revealed by Simultaneous Single-Molecule Optical and Ensemble Electrical Recording
Topic: Life Sciences Research
Published: 2/3/2004
Authors: E L Chandler, A L Smith, Lisa K Burden, John J Kasianowicz, D L Burden
Abstract: We demonstrate a method for simultaneous real-time electrical and single-molecule optical recordings of the interactions between single-stranded DNA and nanoscopic pores in planar lipid membranes. Electrophysiological techniques are used to measure ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830405

56. Substrate specificities and excision kinetics of DNA glycosylases involved in base-excision repair of oxidative DNA damage
Topic: Life Sciences Research
Published: 10/29/2003
Author: M Miral Dizdar
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903147

57. Temperature Dependence of the Kinetics of the Acylase Hydrolysis Reaction by Differential Stopped Flow Microcalorimetry
Topic: Life Sciences Research
Published: 10/1/2003
Authors: M Stodeman, Frederick P. Schwarz
Abstract: The rate of the hydrolysis of N-acetyl-L-methionine by porcine acylase I in 0.1 M phosphate buffer, which is reduced by acetate product inhibition, was monitored calorimetrically at 288.4 K, 298.4 K, 308.5 K, and 311.5 K by a differential stopped flo ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830364

58. DNA Base Damage by the Antitumor Agent 3-Amino-1,2,4-Benzotriazine 1,4-Dioxide (Tirapazamine)
Topic: Life Sciences Research
Published: 9/24/2003
Authors: M Birincioglu, Pawel Jaruga, G Chowdhury, H Rodriguez, M Miral Dizdar, K Gates
Abstract: Tirapazamine is a bioreductively activated DNA-damaging agent that selectively kills the hypoxic cells found in solid tomors. This compound shows great clinical promise and is currently being examined in a variety of clinical trails, including sever ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830363

59. Crystal Structure of the YchF Protein Reveals Binding Sites for GTP and Nucleic Acid
Topic: Life Sciences Research
Published: 7/1/2003
Authors: A Teplyakov, G Obmolova, S Y Chu, J Toedt, E Eisenstein, A J Howard, G L. Gilliland
Abstract: The bacterial protein encoded by the gene ychF is 1 of 11 universally conserved GTPases, and the only one whose function is unknown. The crystal structure determination of YchF was sought to help with the the functional assignment of the protein. ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830360

60. Genomic DNA of Nostoc Commune (Cyanobacteria) Becomes Covalently Modified During Long-Term (Decades) Desiccation But Is Protected From Oxidative Damage and Degradation
Topic: Life Sciences Research
Published: 6/15/2003
Authors: B Shirkey, N J McMaster, Sue C Smith, D J Wright, H Rodriguez, Pawel Jaruga, M Birincioglu, R F Helm, M Potts
Abstract: Genomic DNA of Nostoc commume (Cyanobacteria) became covalently modified during decades of desiccation. Amplification of gene loci from desiccated cells required pretreatment of DNA with N-phenacylthiazolium bromide; a reagent that cleaves DNA- and ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830392



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