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Topic Area: Life Sciences Research

Displaying records 51 to 60 of 251 records.
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51. The Escherichia coli YdcF binds S-adenosyl-L-methionine and adopts an alpha/beta fold characteristic of nucleotide-utilizing enzymes
Topic: Life Sciences Research
Published: 7/1/2008
Authors: Kinlin L. Chao, Kap Lim, C Lehmann, V Doseeva, A J Howard, Frederick P. Schwarz, Osnat Herzberg
Abstract: The crystal structure of YdcF at a resolution of 0.18 nm reveals a fold of the adenine nucleotide α hydrolase-like family. Following the structural clues, a nucleotide binding screen was carried out employing isothermal titration calorimetry. Th ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832211

52. Effect of the Distal C162S Mutation on the Energetics of Drug Binding to p38 alpha MAP Kinase
Topic: Life Sciences Research
Published: 1/15/2008
Authors: Niya Todorova, V Doseeva, Jayanthi Ramprakash, Frederick P. Schwarz
Abstract: The binding constant, Kb, and the binding enthalpy (DbHo) of the inhibitor drugs, SB 203580, SKF 86002, and p38 INH.1 binding to two splice variants of p38a MAP kinase and their C162S mutants in 5mM HEPES buffer at pH =7.4 were determined from isoth ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830550

53. Epitope Selection of Monoclonal Antibodies for Interleukin-4 Quantification Using Suspension Arrays and Forward-Phase Protein Microarrays
Topic: Life Sciences Research
Published: 12/6/2007
Authors: Lili Wang, Kenneth D Cole, Hua-Jun He, Alexander W Peterson, Adolfas Kastytis Gaigalas, Y Zong
Abstract: A recombinant mouse interleukin-4 (IL-4) and three different purified rat anti-mouse IL-4 monoclonal antibodies (Mab) with different epitopes were employed as a model system. This system was used to examine monoclonal antibody effectiveness using bot ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830554

54. A Mycobacterium tuberculosis-derived Lipid Inhibits Membrane Fusion by Modulating Lipid Membrane Domains
Topic: Life Sciences Research
Published: 12/1/2007
Authors: E Hayakawa, F Tokumasu, G Nardone , A J Jin, Vincent A Hackley, J A Dvorak
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=854194

55. Validation of Novel Optical Imaging Modalities: The Pathologists' View
Topic: Life Sciences Research
Published: 10/1/2007
Authors: W Wells, Peter E. Dr. Barker, C Macaulay, M Novelli, Richard Levenson, J Crawford
Abstract: Recent advances in optical imaging technology have the potential to improve the accuracy of disease detection and predict treatment response. The critical end-point for the success of any new imaging technology is clinical outcome. The following rev ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830491

56. The Role of Water in the Thermodynamics of Drug Binding to Cyclodextrin
Topic: Life Sciences Research
Published: 7/1/2007
Authors: Niya Tordorova, Frederick P. Schwarz
Abstract: The thermodynamic parameters, DBGo, DBHo, and DBSo, of the drugs flurbiprofen (FLP), nabumetone (NAB), and naproxen (NPX) binding to b-cyclodextrin (bCD) and to g-cyclodextrin (gCD) in 0.10 M sodium phosphate buffer were determined from isothermal ti ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830570

57. Human Polymorphic Variants of the NEIL1 DNA Glycosylase: Potential Implications for Human Health
Topic: Life Sciences Research
Published: 5/25/2007
Authors: Laura Roy, Pawel Jaruga, Thomas Wood, Amanda K McCullough, M Miral Dizdar, R. Stephen Lloyd
Abstract: In mammalian cells, the repair of DNA bases damaged by reactive oxygen species is primarily initiated by a series of DNA glycosylases that include OGG1, NTH1, NEIL1 and NEIL2. To explore the functional significance of NEIL1, we recently reported tha ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830533

58. The Stiffness of Collagen Fibrils Influences Vascular Smooth Muscle Cell Phenotype
Topic: Life Sciences Research
Published: 3/1/2007
Authors: Dennis P. McDaniel, Gordon Allan Shaw, John T Elliott, Kiran Bhadriraju, Curtis W Meuse, Koo-Hyun Chung, Anne L Plant
Abstract: Cells receive signals from the extracellular matrix through receptor-dependent interactions, but they are also influenced by the mechanical properties of the matrix. While bulk properties of substrates have been shown to effect cell behavior, we sho ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830514

59. Real-time telomerase assay of less-invasively collected esophageal cell samples
Topic: Life Sciences Research
Published: 11/28/2006
Authors: Brenna M McGruder, Donald H Atha, Wendy Wang, Konrad Huppi, Wen-Qiang Wei, Christian C Abnet, You-Lin Qiao, Sanford M Dawsey, Philip R Taylor, J P. Jakupciak
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906444

60. Repair of Formamidopyrimidines in DNA Involves Different Glycosylases - Role of the OGG1, NTH1, and NEIL1 Enzymes
Topic: Life Sciences Research
Published: 12/9/2005
Authors: J Hu, N de Souza-Pinto, K Haraguchi, Barbara A Hogue, Pawel Jaruga, M M Greenberg, M Miral Dizdar, V. Bohr
Abstract: 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 4,6-diamino-5-formamidopyrimidine (FapyA), are formed abundantly in DNA of cultured cells or tissues exposed to ionizing radiation or to other free radical-generating systems. We show here that ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830498



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