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Topic Area: Life Sciences Research

Displaying records 41 to 50 of 272 records.
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41. Predicting Segmentation Accuracy for Biological Cell Images
Topic: Life Sciences Research
Published: 11/29/2010
Authors: Adele P Peskin, Alden A Dima, Joe Chalfoun
Abstract: We have performed image segmentations on a very large number of images, using a wide variety of imaging conditions and cell lines, in order to study trends in the segmentation results and make predictions about segmentation accuracy. Comparing result ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904744

42. A Human Inspired Local Ratio-Based Algorithm for Edge Detection in Fluorescent Cell Images
Topic: Life Sciences Research
Published: 11/25/2010
Authors: Adele P Peskin, Joe Chalfoun, Alden A Dima, John T Elliott, James J Filliben
Abstract: We have developed a new semi-automated method for segmenting images of biological cells seeded at low density on tissue culture substrates, which we use to improve the generation of reference data for the evaluation of automated segmentation algor ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906723

43. Depletion of Abundant Plasma Proteins and Limitations of Plasma Proteomics
Topic: Life Sciences Research
Published: 10/1/2010
Authors: Chengjian Tu, Paul A Rudnick, Misti Y. Martinez, Kristin L. Cheek, Stephen E Stein, Robert J. Slebos, Daniel C. Liebler
Abstract: Immunoaffinity depletion of high abundance plasma proteins is frequently employed to enhance detection of lower abundance proteins in both shotgun and targeted proteomic analyses. Here we present a detailed evaluation of the Agilent Multiple Affinit ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905138

44. Microfluidic Based Contactless DielectrophoreticDevice: Modeling and Analysis
Topic: Life Sciences Research
Published: 9/1/2010
Authors: Saugandhika Minnikanti, Darwin R Reyes-Hernandez, Ruben Claudio Aguilar, Joseph Pancrazio, Michael Gaitan, Nathalia Peixoto
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907086

45. Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix
Topic: Life Sciences Research
Published: 7/13/2010
Authors: Alexander W Peterson, Michael W Halter, Alessandro Tona, Kiran Bhadriraju, Anne L Plant
Abstract: Details of cellular interaction with a fibronectin coated surface were examined using surface plasmon resonance imaging (SPRI). SPRI is a label-free highly sensitive technique that is based on the measurement of interfacial refractive index. SPRI is ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903817

46. The mouse ortholog of NEIL3 is a functional DNA glycosylase in vitro and in vivo
Topic: Life Sciences Research
Published: 3/16/2010
Authors: Minin Liu, Viswanath Bandaru, Jeffrey Bond, Pawel Jaruga, Xiaobei Zhao, Plamen P Christov, Cynthia Burrows, Carmelo J. Rizzo, M Miral Dizdar, Susan Wallace
Abstract: To protect cells from oxidative DNA damage and mutagenesis, organisms possess multiple glycosylases to recognize the damaged bases and to initiate the Base Excision Repair (BER) pathway. Recently, three DNA glycosylases were identified in mammals tha ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903370

47. Evidence for the involvement of DNA repair enzyme NEIL1 in nucleotide excision repair of (5'R)- and (5'S)-8,5'-Cyclo-2'-deoxyadenosines
Topic: Life Sciences Research
Published: 2/16/2010
Authors: Pawel Jaruga, Yan Xiao, Vladimir Vartanian, R Stephen Lloyd, M Miral Dizdar
Abstract: The DNA repair enzyme NEIL1 is a DNA glycosylase that is involved in the first step of base excision repair (BER) of oxidatively induced DNA damage. NEIL1 exhibits a strong preference for excision of 4,6-diamino-5-formamidopyrimidine (FapyAde) and 2, ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904755

48. The oxidative DNA glycosylases of Mycobacterium tuberculosis exhibit different substrate specificities from their Escherichia coli counterparts
Topic: Life Sciences Research
Published: 2/4/2010
Authors: Yin Guo, Viswanath Bandaru, Pawel Jaruga, Xiaobei Zhao, Cynthia Burrows, Shigenori Iwai, M Miral Dizdar, Jeffrey Bond, Susan Wallace
Abstract: The DNA glycosylases function in the first step of the base excision repair process that is responsible for removing endogenous oxidative purine and pyrimidine damages from DNA. The DNA glycosylases that remove oxidized DNA bases fall into two genera ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904173

49. In-plane Homogeneity and Lipid Dynamics in tethered Bilayer Lipid Membranes (tBLMs)
Topic: Life Sciences Research
Published: 1/1/2010
Authors: Siddharth Shenoy, Radu Moldovan, James Fitzpatrick, David J Vanderah, Markus Deserno, Mathias Losche
Abstract: Tethered bilayer lipid membranes (tBLMs) were prepared by the self-assembly of thiolated lipidic anchors on gold films sputtered on Si wafers, followed by phospholipid precipitation via rapid solvent exchange. They were characterized in their in-plan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903881

50. Quantitative Infrared Spectroscopy of Formalin-Fixed, Paraffin-Embedded Tissue Specimens: Paraffin Wax Removal with Organic Solvents
Report Number: 832407
Topic: Life Sciences Research
Published: 12/1/2009
Authors: Curtis W Meuse, Peter E. Dr. Barker
Abstract: Formalin-fixed, paraffin embedded tissue specimens for histopathology are cut to 3-20 μm thickness to allow transmission of light, and optimize tissue morphology. Tissue is stabilized by formalin fixation followed by impregnation with wax in non ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832407



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