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Topic Area: Life Sciences Research

Displaying records 21 to 30 of 266 records.
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21. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Topic: Life Sciences Research
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

22. Fabrication of Microelectrodes on Polyester Membranes for Dielectrophoretic Cell Capture
Topic: Life Sciences Research
Published: 6/18/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: We present a new system for cell capturing on permeable polyester (PET) membranes using dielectrophoretic forces. For the first time gold microelectrodes were fabricated on PET membranes using conventional photolithographic techniques. Their charac ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911140

23. Characterization of the DNA primase complex isolated from the archaeon, Thermococcus kodakaraensis.
Topic: Life Sciences Research
Published: 5/11/2012
Authors: Zvi Kelman, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan
Abstract: In most organisms, DNA replication is initiated by DNA primases which synthesize primers that are elongated by DNA polymerases. In this report we describe the isolation and biochemical characterization of the DNA primase complex and its subunits from ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910426

24. Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakaraensis DNA primase
Topic: Life Sciences Research
Published: 5/11/2012
Authors: Zvi Kelman, John P Marino, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan, Gary Giulian, Wei Yuan, Shuwei Li
Abstract: In the presence of dATP, glycerol and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis (Tk) catalyzed the formation of two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the Tk p41 cat ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910427

25. Challenges in Microbial Sampling in the Indoor Environment
Series: Technical Note (NIST TN)
Report Number: 1737
Topic: Life Sciences Research
Published: 3/1/2012
Authors: Jayne B Morrow, Autumn S. Downey, Jordan Peccia
Abstract: This report summarizes the results of the Challenges in Microbial Sampling in the Indoor Environment Workshop held on February 14-15, 2011 at the National Institute of Standards and Technology in Gaithersburg, Maryland.
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910577

26. Mathematical Model Formulation and Validation of Water and Cryoprotective Agent Transport in Whole Hamster Pancreatic Islets
Topic: Life Sciences Research
Published: 10/11/2011
Authors: James D. Benson, Charles T Benson, John K Critser
Abstract: A mathematical and conceptual model of water and solute transport for whole hamster pancreatic islets has been developed and experimentally validated incorporating fundamental biophysical data from previous studies on individual hamster islet cells ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906347

27. Segmentation and Cell Tracking of Breast Cancer Cells
Topic: Life Sciences Research
Published: 9/26/2011
Authors: Adele P Peskin, Daniel J. Hoeppner, Christina H. Stuelten
Abstract: We describe a new technique to segment and track the cell images of a breast cancer cell line in order to study cell migration and metastasis. Within each image cell phenotypes vary widely, ranging from very bright completely bounded cells to barely ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908649

28. Thermococcus kodakarensis has two functional PCNA homologues but only one is essential for viability
Topic: Life Sciences Research
Published: 8/5/2011
Author: Zvi Kelman
Abstract: Proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911331

29. Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture
Topic: Life Sciences Research
Published: 5/2/2011
Authors: Kaushik Chatterjee, Marian F Young, Carl George Simon Jr
Abstract: Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903763

30. A General Model for the Dynamics of Cell Volume, Global Stability, and Optimal Control
Topic: Life Sciences Research
Published: 2/11/2011
Author: James D. Benson
Abstract: Cell volume and concentration regulation in the presence of changing extracellular environments has been studied for centuries, and recently a general nondimensional model was introduced that encompassed solute and solvent transmembrane flux for a wi ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904647



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