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Topic Area: Life Sciences Research

Displaying records 21 to 30 of 271 records.
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21. Thermococcus kodakarensis DNA replication
Topic: Life Sciences Research
Published: 2/2/2013
Authors: Zvi Kelman, Lori Kelman, Zhuo Li
Abstract: DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began about twenty years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This h ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912749

22. Next-generation Sequencing: Guidance for the Translation from Research to Clinical Applications
Topic: Life Sciences Research
Published: 11/8/2012
Authors: Justin M Zook, Amy Gargis, Lisa Kalman, Ira Lubin
Abstract: We would like to draw your readers‰ attention to the Next-generation Sequencing: Standardization of Clinical Testing (Nex-StoCT) guidelines, which represent an initial step toward ensuring that the results derived from next-generation sequencing (N ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910560

23. Remote sensing of chiral signatures on Mars
Topic: Life Sciences Research
Published: 11/1/2012
Authors: William Sparks, James H. Hough, Thomas Avery Germer, Frank Robb, Ludmilla Kolokolova
Abstract: We describe circular polarization as a remote sensing diagnostic of chiral signatures which may be applied to Mars. The remarkable phenomenon of homochirality provides a unique biosignature which can be amenable to remote sensing through circular ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910692

24. GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES
Topic: Life Sciences Research
Published: 10/28/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911771

25. Characterization of In Vitro Transcription Amplification Linearity and Variability in the Low Copy Number Regime using ERCC Spike Ins
Topic: Life Sciences Research
Published: 10/20/2012
Authors: Jason G Kralj, Marc L Salit
Abstract: Background Characterization of whole transcriptomes from small RNA samples (less than 1 ng total RNA) has been a major challenge. Typically, samples are extracted and purified, amplified by in vitro transcription (IVT), and then subjected to genome- ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=909936

26. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Topic: Life Sciences Research
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

27. Fabrication of Microelectrodes on Polyester Membranes for Dielectrophoretic Cell Capture
Topic: Life Sciences Research
Published: 6/18/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: We present a new system for cell capturing on permeable polyester (PET) membranes using dielectrophoretic forces. For the first time gold microelectrodes were fabricated on PET membranes using conventional photolithographic techniques. Their charac ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911140

28. Characterization of the DNA primase complex isolated from the archaeon, Thermococcus kodakaraensis.
Topic: Life Sciences Research
Published: 5/11/2012
Authors: Zvi Kelman, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan
Abstract: In most organisms, DNA replication is initiated by DNA primases which synthesize primers that are elongated by DNA polymerases. In this report we describe the isolation and biochemical characterization of the DNA primase complex and its subunits from ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910426

29. Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakaraensis DNA primase
Topic: Life Sciences Research
Published: 5/11/2012
Authors: Zvi Kelman, John P Marino, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan, Gary Giulian, Wei Yuan, Shuwei Li
Abstract: In the presence of dATP, glycerol and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis (Tk) catalyzed the formation of two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the Tk p41 cat ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910427

30. Challenges in Microbial Sampling in the Indoor Environment
Series: Technical Note (NIST TN)
Report Number: 1737
Topic: Life Sciences Research
Published: 3/1/2012
Authors: Jayne B Morrow, Autumn S. Downey, Jordan Peccia
Abstract: This report summarizes the results of the Challenges in Microbial Sampling in the Indoor Environment Workshop held on February 14-15, 2011 at the National Institute of Standards and Technology in Gaithersburg, Maryland.
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910577



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