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You searched on: Topic Area: Life Sciences Research

Displaying records 191 to 200 of 273 records.
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191. Graphical Tools for RFLP Measurement Quality Assurance: Laboratory Performance Charts
Topic: Life Sciences Research
Published: 9/1/1999
Authors: K T. Gary, David Lee Duewer, D J. Reeder
Abstract: Forensic restriction fragment length polymorphism analyses typically provide two band size results at each genetic locus for each sample. In collaboration with the member laboratories of the Technical Working Group for DNA Analysis Methods, we have ...

192. Folding and Assembly in Rhodopsin: Effect of Mutations in the Sixth Transmembrane Helix of the Third Cytoplasmic Loop
Topic: Life Sciences Research
Published: 7/23/1999
Authors: K D. Ridge, T Ngo, S S. Lee, N G Abdulaev
Abstract: Previous studies on bovine opsin folding and assembly have identified an amino-terminal fragment, EF(1-232), which folds and inserts into a membrane only after coexpression with its complementary carboxyl-terminal fragment, EF(233-348). To furthe ...

193. Supported Hybrid Bilayer Membranes as Rugged Cell Membrane Mimics
Topic: Life Sciences Research
Published: 7/20/1999
Author: Anne L Plant

194. Kinetic Characterization of Chiral Biocatalysis of Cycloarenes by the Camphor 5-Monooxygenase Enzyme System
Topic: Life Sciences Research
Published: 6/15/1999
Authors: D A Grayson, V L. Vilker
Abstract: Redox enzyme mediated biocatalysis has the potential to regio - and stereo-specificity oxidive hydrocarbons producing valuable products with minimal by-product formation. In-vitro reactions of the Camphor (cytochrome P-450) 5-monooxygenase enzyme sy ...

195. Detection of p53 Gene Mutation: Analysis by Single-Strand Conformation Polymorphism and Cleavase Fragment Length Polymorphism
Topic: Life Sciences Research
Published: 6/1/1999
Authors: C D O'Connell, Donald H Atha, M C Oldenburg, J Tian, M Siebert, D Handrow, K Grooms, L Heisler, M de Arruda
Abstract: We have generated a collection of clones containing single point mutations within the exon 5-9 hot spot regions of the p53 gene by using polymerase chain reaction (PCR) to amplify select regions of the gene from characterized cell lines. These clone ...

196. The Three-dimensional Structures of Two Isoforms of Nucleoside Diphosphate Kinase from Bovine Retina
Topic: Life Sciences Research
Published: 6/1/1999
Authors: Jane E Ladner, N G Abdulaev, D L Kakuev, M. Tordova, K D. Ridge, G L. Gilliland

197. Crystal Structure of Escherichia coli Uracil DNA Glycosylase and Its Complexes with Uracil and Glycerol: Structure and Glycosylase Mechanism Revisited
Topic: Life Sciences Research
Published: 4/1/1999
Authors: G Xiao, M. Tordova, J. Jagadeesh, A C Drohat, J T. Stivers, G L. Gilliland

198. Oxygen Binding to alpha-Subunits in High-Salt Crystals of T-State Haemoglobin
Topic: Life Sciences Research
Published: 4/1/1999
Authors: G B Vasquez, X Ji, I Pechik, C Fronticelli, G L. Gilliland

199. Effect of Mutations at the Monomer-Monomer Interface of cAMP Receptor Protein on Specific DNA Binding
Topic: Life Sciences Research
Published: 3/12/1999
Authors: Y. Shi, S L Wang, Susan Krueger, Frederick P. Schwarz
Abstract: To Determine the thermodynamic role of binding of an operon to cAMP receptor protein (CRP) in the activation of transcription, isothermal titration calorimetry measurements were performed on the binding of three 40-base pair DNA sequences to the cycl ...

200. Engineering Receptor-Mediated Cytotoxicity Into Human Ribonucleases by Steric Blockade of Inhibitor Interaction
Topic: Life Sciences Research
Published: 3/1/1999
Authors: M Suzuki, S K Saxena, E Boix, R J Prill, V M Vasandani, Jane E Ladner, C Sung, R J Youle
Abstract: Several nonammalian members of the Rnase A superfamily exhibit anticancer activity that appears to correlate with resistance to the cytosolic ribonuclease inhibitor (RI). We mutated two human ribonucleases-pancreatic Rnase (hRNAse) and eosinophil-de ...

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