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Topic Area: Life Sciences Research

Displaying records 191 to 200 of 269 records.
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191. Detection of p53 Gene Mutation: Analysis by Single-Strand Conformation Polymorphism and Cleavase Fragment Length Polymorphism
Topic: Life Sciences Research
Published: 6/1/1999
Authors: C D O'Connell, Donald H Atha, M C Oldenburg, J Tian, M Siebert, D Handrow, K Grooms, L Heisler, M de Arruda
Abstract: We have generated a collection of clones containing single point mutations within the exon 5-9 hot spot regions of the p53 gene by using polymerase chain reaction (PCR) to amplify select regions of the gene from characterized cell lines. These clone ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830063

192. The Three-dimensional Structures of Two Isoforms of Nucleoside Diphosphate Kinase from Bovine Retina
Topic: Life Sciences Research
Published: 6/1/1999
Authors: Jane E Ladner, N G Abdulaev, D L Kakuev, M. Tordova, K D. Ridge, G L. Gilliland
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100589

193. Crystal Structure of Escherichia coli Uracil DNA Glycosylase and Its Complexes with Uracil and Glycerol: Structure and Glycosylase Mechanism Revisited
Topic: Life Sciences Research
Published: 4/1/1999
Authors: G Xiao, M. Tordova, J. Jagadeesh, A C Drohat, J T. Stivers, G L. Gilliland
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100619

194. Oxygen Binding to alpha-Subunits in High-Salt Crystals of T-State Haemoglobin
Topic: Life Sciences Research
Published: 4/1/1999
Authors: G B Vasquez, X Ji, I Pechik, C Fronticelli, G L. Gilliland
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100617

195. Effect of Mutations at the Monomer-Monomer Interface of cAMP Receptor Protein on Specific DNA Binding
Topic: Life Sciences Research
Published: 3/12/1999
Authors: Y. Shi, S L Wang, Susan Krueger, Frederick P. Schwarz
Abstract: To Determine the thermodynamic role of binding of an operon to cAMP receptor protein (CRP) in the activation of transcription, isothermal titration calorimetry measurements were performed on the binding of three 40-base pair DNA sequences to the cycl ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830066

196. Engineering Receptor-Mediated Cytotoxicity Into Human Ribonucleases by Steric Blockade of Inhibitor Interaction
Topic: Life Sciences Research
Published: 3/1/1999
Authors: M Suzuki, S K Saxena, E Boix, R J Prill, V M Vasandani, Jane E Ladner, C Sung, R J Youle
Abstract: Several nonammalian members of the Rnase A superfamily exhibit anticancer activity that appears to correlate with resistance to the cytosolic ribonuclease inhibitor (RI). We mutated two human ribonucleases-pancreatic Rnase (hRNAse) and eosinophil-de ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830058

197. Genetically Engineered Metal Ion Binding Sites on the Outside of a Channel's Transmembrane {Beta}-Barrel
Topic: Life Sciences Research
Published: 2/1/1999
Authors: John J Kasianowicz, D L Burden, L C Han, S Cheley, H Bayley
Abstract: We are exploring the ability of genetically engineered versions of the Staphlococcus aureus {alpha}HL} ion channel to serve as rationally designed sensor components for analytes including divalent cations. We show here that neither the hemolytic act ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830040

198. Electronic Structure and Spectra of Oxovanadium Silicate
Topic: Life Sciences Research
Published: 1/11/1999
Author: Morris Krauss
Abstract: The electronic structure and spectroscopy of the oxovanadium catalytic center on a silica surface is modeled with the molecule OV(OSIH^d3^)3. Excited electronic states are examined at optimized geometries for the ground singlet and triplet states. ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=831064

199. Cysteines beta 93 and beta 112 as Probes of Conformational and Functional Events at the Human Hemoglobin Subunit Interfaces
Topic: Life Sciences Research
Published: 1/1/1999
Authors: G B. Vasquez, M Karavitis, X Ji, I Pechik, W S Brinigar, G L. Gilliland, C Fronticelli
Abstract: Human hemoglobin is a tetrameric protein containing two types of subunits, [alpha] and [Beta]. They assemble forming two different interfaces. The [alpha^d1^[Beta}^d1^ (and [alpha]^d2^[Beta]^d2^) interface stabilize the two dimers and is not modifi ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830036

200. Self Assembly Driven by Hydrophobic Interaction at Alkanethiol Monolayers: Mechanism of Formation of Hybrid Bilayer Membranes
Topic: Life Sciences Research
Published: 12/14/1998
Authors: J B Hubbard, Vitalii Ivanovich Silin, Anne L Plant
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100587



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