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Topic Area: Life Sciences Research

Displaying records 181 to 190 of 270 records.
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181. Thermal Stability of PNA/DNA and DNA/DNA Duplexes by Differential Scanning Calorimetry
Topic: Life Sciences Research
Published: 12/15/1999
Authors: M C. Chakrabarti, Frederick P. Schwarz
Abstract: Thermodynamic quanties describing the hybridizatin reactions for 8mer PNA/DNA duplexes and the corresponding 8mer DNA/DNA duplexes were determined by isothermal titation calorimetry measurements at ambient temperatures. Values of {delta}G^db^ for ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830256

182. Long-lifetime Lipid Rhenium Metal-ligand Complex for Probing Membrane Dynamics on the Microsecond Timescale
Topic: Life Sciences Research
Published: 12/1/1999
Authors: L. Li, F N Castellano, I Gryczynski, J R Lakowicz
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100593

183. Mapping Oxidative DNA Damage and Mechanisms of Repair
Topic: Life Sciences Research
Published: 12/1/1999
Authors: H Rodriguez, T O'connor, S. A. Akman
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100606

184. Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules
Topic: Life Sciences Research
Published: 12/1/1999
Authors: M Akeson, D Branton, John J Kasianowicz, E Brandin, D W Deamer
Abstract: Single molecules of DNA or RNA can be detected as they are driven through a single alpha-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential sin ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830074

185. The Effect of Experimental Conditions on the Levels of Oxidatively Modified Bases in DNA as Measured by Gas Chromatography- Mass Spectrometry. How Many Modified Bases Are Involved? Prepurification or Not?
Topic: Life Sciences Research
Published: 12/1/1999
Authors: S. Senturker, M Miral Dizdar
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100608

186. Challenges in Capturing Oxygenase Activity In Vitro
Topic: Life Sciences Research
Published: 11/1/1999
Authors: V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J Holden
Abstract: Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large clas ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830098

187. Heteronuclear NMR and Crystallographic Studies of Wild-Type and H187Q Escherichia Coli Uracil DNA Glycosylase: Electrophilic Catalysis of Uracil Expulsion by a Neutral Histidine 187
Topic: Life Sciences Research
Published: 9/14/1999
Authors: A C Drohat, G Xiao, M Tordova, J. Jagadeesh, K Pankiewicz, K A Watanbe, G L. Gilliland, J T. Stivers
Abstract: The nature of the putative general acid, His187, in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830100

188. Graphical Tools for RFLP Measurement Quality Assurance: Laboratory Performance Charts
Topic: Life Sciences Research
Published: 9/1/1999
Authors: K T. Gary, David Lee Duewer, D J. Reeder
Abstract: Forensic restriction fragment length polymorphism analyses typically provide two band size results at each genetic locus for each sample. In collaboration with the member laboratories of the Technical Working Group for DNA Analysis Methods, we have ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830052

189. Folding and Assembly in Rhodopsin: Effect of Mutations in the Sixth Transmembrane Helix of the Third Cytoplasmic Loop
Topic: Life Sciences Research
Published: 7/23/1999
Authors: K D. Ridge, T Ngo, S S. Lee, N G Abdulaev
Abstract: Previous studies on bovine opsin folding and assembly have identified an amino-terminal fragment, EF(1-232), which folds and inserts into a membrane only after coexpression with its complementary carboxyl-terminal fragment, EF(233-348). To furthe ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830083

190. Supported Hybrid Bilayer Membranes as Rugged Cell Membrane Mimics
Topic: Life Sciences Research
Published: 7/20/1999
Author: Anne L Plant
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=100601



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