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Topic Area: Life Sciences Research

Displaying records 181 to 190 of 266 records.
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181. The Effect of Experimental Conditions on the Levels of Oxidatively Modified Bases in DNA as Measured by Gas Chromatography- Mass Spectrometry. How Many Modified Bases Are Involved? Prepurification or Not?
Topic: Life Sciences Research
Published: 12/1/1999
Authors: S. Senturker, M Miral Dizdar

182. Challenges in Capturing Oxygenase Activity In Vitro
Topic: Life Sciences Research
Published: 11/1/1999
Authors: V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J Holden
Abstract: Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large clas ...

183. Heteronuclear NMR and Crystallographic Studies of Wild-Type and H187Q Escherichia Coli Uracil DNA Glycosylase: Electrophilic Catalysis of Uracil Expulsion by a Neutral Histidine 187
Topic: Life Sciences Research
Published: 9/14/1999
Authors: A C Drohat, G Xiao, M Tordova, J. Jagadeesh, K Pankiewicz, K A Watanbe, G L. Gilliland, J T. Stivers
Abstract: The nature of the putative general acid, His187, in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with ...

184. Graphical Tools for RFLP Measurement Quality Assurance: Laboratory Performance Charts
Topic: Life Sciences Research
Published: 9/1/1999
Authors: K T. Gary, David Lee Duewer, D J. Reeder
Abstract: Forensic restriction fragment length polymorphism analyses typically provide two band size results at each genetic locus for each sample. In collaboration with the member laboratories of the Technical Working Group for DNA Analysis Methods, we have ...

185. Folding and Assembly in Rhodopsin: Effect of Mutations in the Sixth Transmembrane Helix of the Third Cytoplasmic Loop
Topic: Life Sciences Research
Published: 7/23/1999
Authors: K D. Ridge, T Ngo, S S. Lee, N G Abdulaev
Abstract: Previous studies on bovine opsin folding and assembly have identified an amino-terminal fragment, EF(1-232), which folds and inserts into a membrane only after coexpression with its complementary carboxyl-terminal fragment, EF(233-348). To furthe ...

186. Supported Hybrid Bilayer Membranes as Rugged Cell Membrane Mimics
Topic: Life Sciences Research
Published: 7/20/1999
Author: Anne L Plant

187. Kinetic Characterization of Chiral Biocatalysis of Cycloarenes by the Camphor 5-Monooxygenase Enzyme System
Topic: Life Sciences Research
Published: 6/15/1999
Authors: D A Grayson, V L. Vilker
Abstract: Redox enzyme mediated biocatalysis has the potential to regio - and stereo-specificity oxidive hydrocarbons producing valuable products with minimal by-product formation. In-vitro reactions of the Camphor (cytochrome P-450) 5-monooxygenase enzyme sy ...

188. Detection of p53 Gene Mutation: Analysis by Single-Strand Conformation Polymorphism and Cleavase Fragment Length Polymorphism
Topic: Life Sciences Research
Published: 6/1/1999
Authors: C D O'Connell, Donald H Atha, M C Oldenburg, J Tian, M Siebert, D Handrow, K Grooms, L Heisler, M de Arruda
Abstract: We have generated a collection of clones containing single point mutations within the exon 5-9 hot spot regions of the p53 gene by using polymerase chain reaction (PCR) to amplify select regions of the gene from characterized cell lines. These clone ...

189. The Three-dimensional Structures of Two Isoforms of Nucleoside Diphosphate Kinase from Bovine Retina
Topic: Life Sciences Research
Published: 6/1/1999
Authors: Jane E Ladner, N G Abdulaev, D L Kakuev, M. Tordova, K D. Ridge, G L. Gilliland

190. Crystal Structure of Escherichia coli Uracil DNA Glycosylase and Its Complexes with Uracil and Glycerol: Structure and Glycosylase Mechanism Revisited
Topic: Life Sciences Research
Published: 4/1/1999
Authors: G Xiao, M. Tordova, J. Jagadeesh, A C Drohat, J T. Stivers, G L. Gilliland

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