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Topic Area: Diagnostics
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Displaying records 1 to 10 of 25 records.
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1. Determining carbapenemase activity with 18O labeling and targeted mass spectrometry
Topic: Diagnostics
Published: 10/16/2013
Authors: Meiyao Wang, Yang Shen, Illarion V Turko, Daniel Nelson, Shuwei Li
Abstract: Carbapenems are broad spectrum antibiotics considered as last resort drugs to treat bacterial infections. Carbapenem-hydrolyzing β-lactamases (also called carbapenemases), however, can confer bacterial resistance and represent a serious health t ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914525

2. Quantitative Analysis of Dendron-Conjugated Cisplatin-Complexed Gold Nanoparticles Using Scanning Particle Mobility Mass Spectrometry
Topic: Diagnostics
Published: 5/8/2013
Authors: De-Hao D. Tsai, Tae Joon Cho, Sherrie R. Elzey, Julien C. Gigault, Vincent A Hackley
Abstract: We report a high-resolution and traceable method to quantify the drug loading on nanoparticle-based cancer therapeutics, and demonstrate this method using a model cisplatin functionalized dendron-gold nanoparticle (AuNP) conjugate. Electrospray diffe ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912782

3. Breast Cancer Biomarker Measurements and Standards
Topic: Diagnostics
Published: 1/24/2013
Authors: Kenneth D Cole, Lili Wang, Hua-Jun He
Abstract: Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=912867

4. GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES
Topic: Diagnostics
Published: 10/28/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911771

5. Characterization of In Vitro Transcription Amplification Linearity and Variability in the Low Copy Number Regime using ERCC Spike Ins
Topic: Diagnostics
Published: 10/20/2012
Authors: Jason G Kralj, Marc L Salit
Abstract: Background Characterization of whole transcriptomes from small RNA samples (less than 1 ng total RNA) has been a major challenge. Typically, samples are extracted and purified, amplified by in vitro transcription (IVT), and then subjected to genome- ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=909936

6. Simple device for rare cell capture from whole blood
Topic: Diagnostics
Published: 9/26/2012
Authors: Jason G Kralj, Samuel P Forry, Matthew S Munson, Thomas P Forbes, Chandamany Arya, Lynn Sorbara, Alessandro Tona, Sudhir Srivastava
Abstract: We have developed a system to isolate rare cells from whole blood using commercially available components and simple microfluidics that can provide biologists with the capabilities of the monolithic devices. We characterized the capture of MCF-7 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911536

7. Determination of Moisture Content of Single-Wall Carbon Nanotubes
Topic: Diagnostics
Published: 10/17/2011
Authors: Rolf Louis Zeisler, Rabia Oflaz, Ralph E. Sturgeon, Rick L Paul, Brian E Lang, Jeffrey A Fagan, Joseph W Lam, Anthony Windust, P Grinberg, Benoit Simard, Christopher T. Kingston
Abstract: Several techniques were evaluated for the establishment of reliable water/moisture content of single-wall carbon nanotubes. Karl Fischer titration (KF) provides for a direct measure of the water content and was used for benchmarking against results ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908873

8. Cell Adhesion to Borate Glasses by Colloidal Probe Microscopy
Topic: Diagnostics
Published: 8/1/2011
Authors: Sheldon Martin Wiederhorn, Carl George Simon Jr, Young-Hun Chae
Abstract: The adhesion of Osteoblast-like cells to silicate and borate glasses was measured using colloidal probe microscopy in a cell growth medium. The probe consisted of silicate and borate glass spheres, 25 µm to 50 µm in diameter, attached to the tip of a ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904608

9. Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay
Topic: Diagnostics
Published: 5/15/2011
Authors: Gregory A Cooksey, John T Elliott, Anne L Plant
Abstract: Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content cell-based assays. However, maximizing the value of these assays for drug discovery, screening or toxicity evaluation, for example, will require va ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907416

10. Recent Advances in Electrochemical Glyco-biosensing
Topic: Diagnostics
Published: 4/8/2011
Authors: Rebecca A Zangmeister, Germarie Sanchez-Pomales
Abstract: Biosensors based on electrochemical transduction mechanisms have recently made advances into the field of glycan analysis. These glyco-biosensor assays offer simple, rapid, sensitive and economical approaches to the measurement need for rapid glycan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908197



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