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Author: John Marino

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1. Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy
Published: 1/2/2014
Authors: Steve Y. Rhieu, Aaron A Urbas, Daniel W Bearden, John P Marino, Vytautas Reipa, Katrice A Lippa
Abstract: Non-invasive and real-time analysis of cellular redox processes has been greatly hampered by lack of suitable measurement techniques. Here we describe an in-cell nuclear magnetic resonance (NMR)-based method for measuring the intracellular glutathion ...

2. Structural Characterization of the Viral and Complementary RNA Panhandle Motifs of Infectious Salmon Anemia Virus
Published: 12/1/2011
Authors: Robert G Brinson, Andrea L Szakal, John P Marino
Abstract: Infectious Salmon Anemia Virus (ISAV), an RNA virus of negative polarity in the family orthomyxoviridae, has emerged as a virus of great concern to the aquaculture industry, as it can lead to highly contagious and lethal infections in farm-raised sal ...

3. Dissecting Structural Transitions in the HIV-1 Dimerization Initiation Site RNA using 2-aminopurine fluorescence
Published: 10/1/2009
Authors: Hui-wen Lee, Katharine T. Briggs, John P Marino
Abstract: A highly conserved 35 nucleotide RNA stem-loop, the dimerization initiation site (DIS), in the 5 untranslated region (UTR) of the human immunodeficiency virus type I (HIV-1) genome has been identified as the sequence primarily responsible for initia ...

4. Probing Anomalous Structural Features in Polypurine Tract-Containing RNA-DNA Hybrids with Neomycin B
Published: 7/28/2009
Authors: Robert G Brinson, Kevin Turner, Hye Young Yi-Brunozzi, Stuart F. Le Grice, Daniele Fabris, John P Marino
Abstract: During (-)-strand DNA synthesis in retroviruses and Saccharomyces cerevisiae LTR-retrotransposons, a purine rich region of the RNA template, known as the polypurine tract (PPT) is resistant to RNase H-mediated hydrolysis and subsequently serves as a ...

5. NMR Analysis of Rhodopsin-Transducin Interactions
Published: 12/1/2006
Authors: Kevin Ridge, John P Marino, T Ngo, Eva Ramon, D M Brabazon, N G Abdulaev
Abstract: Heterotrimeric G-protein activation by an agonist-stimulated G-protein coupled receptor (R*) requires the propagation of structural signals from the receptor interacting surfaces to the guanine nucleotide-binding pocket. Employing high-resolution NM ...

6. Conformational Changes Associated With Receptor-Stimulated Guanine Nucleotide Exchange in a Heterotrimeric G-Protein {alpha}-Subunit: NMR Analysis of the GTP{gamma}S-Bound States
Published: 3/17/2006
Authors: K D. Ridge, N G Abdulaev, Xi-Cheng Zhang, T Ngo, D M Brabazon, John P Marino
Abstract: Solution NMR studies of a 15N-labeled G-protein ?-subunit (G?) chimera (15N-ChiT) reconstituted heterotrimer have previously shown that binding of ??-subunits induces a pre-activated conformation in G? that may facilitate interaction with R* and gu ...

7. Examining Ty3 Polypurine Tract Structure and Function by Nucleoside Analog Interference
Published: 2/1/2006
Authors: Chandravanu Dash, John P Marino, Stuart F. Le Grice
Abstract: Although retroviral and LTR-retrotransposon PPTs differ significantly in both their length and sequence, they are accurately removed from (+) RNA and (+) DNA by their cognate RTs, suggesting common structural features of these RNA/DNA hybrids contrib ...

8. A Ribose Sugar Conformational Switch in the LTR-Retrotransposon Ty3 Polypurine Tract-Containing RNA/DNA Hybrid
Published: 11/30/2005
Authors: Hye Young Yi-Brunozzi, D M Brabazon, Daniela Lener, Stuart F J LeGrice, John P Marino
Abstract: To probe structural features of a polypurine-tract (PPT) that mediate its specific recognition and processing, a model 20bp RNA/DNA duplex, which includes the full PPT sequence of the saccharomyces cerevisiae LTR-retrotransposon Ty3, has been investi ...

9. Heterotrimeric G-protein alpha-Subunit Adopts a "Preactivated" Conformation When Associated with beta-Subunits
Published: 8/29/2005
Authors: N G Abdulaev, T Ngo, C Zhang, A Dinh, D M Brabazon, K D Ridge, John P Marino

10. Bacterial Expression and One-Step Purification of an Isotope-Labeled Heterotrimeric G-Protein alpha-Subunit
Published: 5/1/2005
Authors: N G Abdulaev, Xi-Cheng Zhang, Andy Dinh, T Ngo, Phillip Bryan, D M Brabazon, John P Marino, Kevin Ridge
Abstract: Heterologous expression systems are often employed to generate sufficient quantities of isotope-labeled proteins for high-resolution NMR studies. Recently, the interaction between the prodomain region of subtilisin and an active, mutant form of the ...

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