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Author: Darwin Reyes-Hernandez

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1. GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES
Published: 10/28/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911771

2. Enabling Long-Term Dielectrophoretic Actuation for Cell Manipulation and Analysis in Microfluidic Biochips
Published: 10/7/2012
Author: Darwin R Reyes-Hernandez
Abstract: An approach that combines not only the cell manipulation capabilities of dielectrophoresis (DEP), but also the conditions to promote appropriate cell function during long-term experiments is presented. The integration of a hybrid cell adhesive mater ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911615

3. Fabrication of Microelectrodes on Polyester Membranes for Dielectrophoretic Cell Capture
Published: 6/18/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: We present a new system for cell capturing on permeable polyester (PET) membranes using dielectrophoretic forces. For the first time gold microelectrodes were fabricated on PET membranes using conventional photolithographic techniques. Their charac ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911140

4. Dielectrophoretic Cell Capture on Polyester Membranes
Published: 4/2/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A new system for dielectrophoretic cell capture on porous polyester (PET) membranes is presented. Conventional photolithographic techniques were used to fabricate gold microelectrodes on a PET membrane. We characterized these electrodes to proof th ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910623

5. Hybrid Cell Adhesive Material for Instant Dielectrophoretic Cell Trapping and Long Term Cell Function Assessment
Published: 8/16/2011
Authors: Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
Abstract: We present the development and evaluation of an engineered hybrid cell adhesive surface for instant dielectrophoretic trapping and patterning of cells in a microfluidic device. This work demonstrates that a hybrid cell adhesive material (hCAM) prepar ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906647

6. Trapping and Release of Citrate-Capped Gold Nanoparticles
Published: 8/1/2011
Authors: Darwin R Reyes-Hernandez, Geraldine I. Mijares, Brian Joseph Nablo, Kimberly A Briggman, Michael Gaitan
Abstract: An electrical method to trap and release charged gold nanoparticles on and from the surface of alkanethiol self-assembled-monolayer (SAM) modified gold electrodes is presented. Gold nanoparticle (GNP) coated electrodes were formed by immersing amine ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904039

7. Instantaneous Trapping and Long Term Cell Survival Under Dielectrophoretic Conditions Using a Hybrid Cell Adhesive Surface
Published: 10/3/2010
Authors: Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
Abstract: A dielectrophoresis (DEP) microfluidic device that allows instantaneous trapping of cells for rapid return to cell growth conditions (within 5 minutes) and long-term cell experiments (cell proliferation, induction and differentiation) of embryonic P1 ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906185

8. Microfluidic Based Contactless DielectrophoreticDevice: Modeling and Analysis
Published: 9/1/2010
Authors: Saugandhika Minnikanti, Darwin R Reyes-Hernandez, Ruben Claudio Aguilar, Joseph Pancrazio, Michael Gaitan, Nathalia Peixoto
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907086

9. Quantum Dot FRET Sensor for Enzymatic Activity in Microfluidic Device
Published: 6/22/2010
Authors: Georgeta Crivat, Sandra Maria Da Silva, Darwin R Reyes-Hernandez, Samuel P Forry, Laurie E Locascio, Michael Gaitan, Nitsa Rosenzweig, Zeev Rosenzweig
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907085

10. Polyelectrolyte Multilayer-Treated Electrodes for Real-Time Electronic Sensing Cell Proliferation
Series: Journal of Research (NIST JRES)
Published: 4/1/2010
Authors: Geraldine I. Mijares, Darwin R Reyes-Hernandez, Jon C Geist, Michael Gaitan
Abstract: The feasibility of using and the performance of non-biological polyelectrolyte multilayer (PEM) films to facilitate cell attachment on titanium-tungsten alloy/gold (TiW/Au) electrodes for the detection of cellular responses in cell assays are investi ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=33183