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1. An analytical framework for optimizing variant discovery from personal genomes
Published: 2/25/2015
Authors: Justin M Zook, Gareth Highnam, Jason Wang, Dean Kusler, Vinaya Vijayan, Nir Leibovich, David Mittelman
Abstract: The standardization and performance testing of analysis tools is a prerequisite to widespread adoption of high-throughput sequencing, particularly in the clinic. However, performance testing is currently complicated by the paucity of standards and co ...

2. Best Practices for Evaluating Single Nucleotide Variant Calling Methods for Microbial Genomics
Published: 7/7/2015
Authors: Nathanael D Olson, Steven P Lund, Rebecca Colman, Jeffery T. Foster, Jason W. Sahl, James M. Schupp, Paul Keim, Jayne B Morrow, Marc L Salit, Justin M Zook
Abstract: Innovations in sequencing technologies have allowed biologists to make incredible advances in understanding biological systems. As experience grows, researchers increasingly recognize that analyzing the wealth of data provided by these new sequen ...

3. Disentangling the effects of polymer coatings on silver nanoparticle agglomeration, dissolution, and toxicity to determine mechanisms of nanotoxicity
Published: 9/1/2012
Authors: Justin M Zook, Melissa Danielle Halter, Danielle Cleveland, Stephen E Long
Abstract: Silver nanoparticles (AgNPs) are frequently coated by a variety of polymers, which may affect various intertwined mechanisms of toxicity, including agglomeration and dissolution rate. Here, we measure how citrate, dextran, poly(ethylene glycol) (PEG ...

4. Effects of temperature, acyl chain length, and flow-rate ratio on liposome formation and size in a microfluidic hydrodyanmic focusing device
Published: 3/21/2010
Authors: Justin M Zook, Wyatt N Vreeland
Abstract: Microfluidic hydrodynamic focusing of an alcohol/lipid mixture into a narrow fluid stream by two oblique buffer streams provides a controlled and reproducible method of forming phospholipid bilayer vesicles (i.e., liposomes) with relatively monodispe ...

5. Genomic Reference Materials for Clinical Application
Published: 11/21/2014
Authors: Justin M Zook, Marc L Salit
Abstract: Reference Materials are well-characterized, homogeneous, and stable samples that can be used to understand measurement performance. The Genome in a Bottle Consortium is developing whole human genome DNA Reference Materials from large batches of DNA ...

6. International Interlaboratory Study Comparing Single Organism 16S rRNA Sequencing Data: Going Beyond Consensus Sequence Comparisons
Published: 3/5/2015
Authors: Nathanael D Olson, Justin M Zook, Steven P Lund, Fabiola Rojas-Cornejo, Jim Huggett, Jayne B Morrow
Abstract: The Consultative Committee for Amount of Substance established a working group to advance the metrology of microbial identification and quantification. This study represents the initial step towards international comparability and measurement as ...

7. Magnetic connectors for microfluidic applications
Published: 1/1/2010
Authors: Francisco Javier Atencia, Gregory A Cooksey, Andreas Jahn, Justin M Zook, Wyatt N Vreeland, Laurie E Locascio
Abstract: We present a new type of connector based on a simple idea: using a ring magnet on one side of the microfluidic chip and a disc magnet on the other side to provide the interfacial force necessary to provide a sealed connection between tubing or needle ...

8. Measuring agglomerate size distribution and dependence of localized surface plasmon resonance absorbance on gold nanoparticle agglomerate size using analytical ultracentrifugation
Published: 9/3/2011
Authors: Justin M Zook, Vinayak Rastogi, Robert I. MacCuspie, Athena M Keene, Jeffrey A Fagan
Abstract: Nanoparticles frequently agglomerate when dispersed into relevant biological and environmental media, with the resulting change to the effective size distribution dramatically affecting the potential nanotoxicity and the absorbance for biosensor ...

9. Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV/visible absorbance measurements
Series: Special Publication (NIST SP)
Report Number: 1200-13
Published: 6/3/2015
Authors: Justin M Zook, Robert MacCuspie, Stephen E Long, Elijah J Petersen
Abstract: This special publication is one in a series stemming from the National Nanotechnology Initiative (NNI) Nano-EHS Research Strategy which identified Nanomaterial Measurement Infrastructure as one of the essential areas of research needed in order t ...

10. Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV-Visible absorbance
Published: 8/2/2011
Authors: Justin M Zook, Stephen E Long, Danielle Cleveland, Carly Lay Geronimo, Robert I. MacCuspie
Abstract: Distinguishing the toxic effects of nanoparticles (NPs) themselves from the well-studied toxic effects of their ions is a critical but challenging measurement for nanotoxicity studies and regulation. This measurement is especially difficult for silv ...

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  • SP 250-XX: Calibration Services
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  • SP 300-XX: Precision Measurement and Calibration
  • SP 400-XX: Semiconductor Measurement Technology
  • SP 480-XX: Law Enforcement Technology
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  • SP 823-XX: Integrated Services Digital Network Series