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Author: darwin reyes-hernandez
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1. Polyelectrolyte Multilayers in Microfluidic Systems for Biological Applications
Published: 7/31/2014
Authors: Saugandhika Sarma Minnikanti, Aveek Gangopadhyay, Darwin R Reyes-Hernandez
Abstract: The formation of polyelectrolyte multilayers (PEMs) for the first time two decades ago demonstrating the assembly on charged substrates in a very simple and efficient way have proven to be a reliable method to obtain structures tunable at the nanomet ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=916103

2. The Art in Science of microTAS: the 2013 edition!
Published: 3/11/2014
Authors: Darwin R Reyes-Hernandez, Albert Folch, Harp Minhas, Michael Gaitan
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=915579

3. Dielectrophoretic Trapping of P19 Cells on Indium Tin Oxide based Microelectrode Arrays
Published: 11/8/2013
Authors: Aveek Gangopadhyay, Saugandhika Minnikanti, Darwin R Reyes-Hernandez, Mulpuri V. Rao, Nathalia Peixoto
Abstract: A microfabricated device comprised of a microelectrode array (MEA) and a microfluidic channel is presented here for the purpose of trapping cells using positive dielectrophoresis (DEP). Transparent indium tin oxide (ITO) electrodes are patterned in a ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914569

4. Optical Coherence Tomography for Dimensional Metrology of Lab-on-a-chip devices
Published: 10/27/2013
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: Here we present a new metrology tool for the determination of the dimensions of channels from final product Lab-on-a-chip devices. Using Optical Coherence Tomography (OCT) areas of up to 12 mm x 12 mm can be imaged in less than 1 second, without the ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=914314

5. Metrology for Lab-on-a-Chip Final-Product Devices
Published: 5/12/2013
Authors: Darwin R Reyes-Hernandez, Michael W Halter, Jeeseong Hwang
Abstract: New metrology tools to measure the critical parameters of internal structures in lab on a chip devices are greatly needed in order to develop standard tests for this technology. Here we present a method that combines a custom made optical coherence ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=913496

6. GENERATING CELL CO-CULTURES BY RAPID CELL ADHESION ON OPPOSITE SIDES OF POLYESTER MEMBRANES
Published: 10/28/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cell ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911771

7. Enabling Long-Term Dielectrophoretic Actuation for Cell Manipulation and Analysis in Microfluidic Biochips
Published: 10/7/2012
Author: Darwin R Reyes-Hernandez
Abstract: An approach that combines not only the cell manipulation capabilities of dielectrophoresis (DEP), but also the conditions to promote appropriate cell function during long-term experiments is presented. The integration of a hybrid cell adhesive mater ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911615

8. Fabrication of Microelectrodes on Polyester Membranes for Dielectrophoretic Cell Capture
Published: 6/18/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: We present a new system for cell capturing on permeable polyester (PET) membranes using dielectrophoretic forces. For the first time gold microelectrodes were fabricated on PET membranes using conventional photolithographic techniques. Their charac ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911140

9. Dielectrophoretic Cell Capture on Polyester Membranes
Published: 4/2/2012
Authors: Conni Hanke, Petra S. Dittrich, Darwin R Reyes-Hernandez
Abstract: A new system for dielectrophoretic cell capture on porous polyester (PET) membranes is presented. Conventional photolithographic techniques were used to fabricate gold microelectrodes on a PET membrane. We characterized these electrodes to proof th ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=910623

10. Hybrid Cell Adhesive Material for Instant Dielectrophoretic Cell Trapping and Long Term Cell Function Assessment
Published: 8/16/2011
Authors: Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
Abstract: We present the development and evaluation of an engineered hybrid cell adhesive surface for instant dielectrophoretic trapping and patterning of cells in a microfluidic device. This work demonstrates that a hybrid cell adhesive material (hCAM) prepar ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906647



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