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Author: vytautas reipa

Displaying records 21 to 30 of 51 records.
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21. Temperature-Induced Structural Changes in Putidaredoxin: A Circular Dichroism and UV-VIS Absorption Study
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
Abstract: Putidaredoxin (Pdx) is an 11,400 Da iron-sulfur protein that sequentially transfers two electrons to the cytochrome P450cam during the enzymatic cycle of the stereo specific camphor hydroxylation. We report two transitions in the Pdx UV-VIS absorpt ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830425

22. Temperature-induced structural changes in putidaredoxin: a circular dichroism and UV-VIS absorption study
Published: 6/1/2004
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903187

23. Structural Alterations of the Heme Environment of Cytochrome P450cam and the Y96F Mutant as Deduced by Resonance Raman Spectroscopy
Published: 1/1/2003
Authors: G Niaura, Vytautas Reipa, M P. Mayhew, Marcia J Holden, V L. Vilker
Abstract: Resonance Raman spectroscopy at 2.5 cm-1 resolution was used to probe differences in wild type and Y96F mutant P450cam (CYP101), both with and without bound camphor or styrene substrates. In the substrate-free state, the spin state equilibrium is sh ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830245

24. New Degenerate Metal-Oxide Electrodes for Nearly Reversible Direct Electron Transfer to the Redox Proteins
Published: 4/1/2002
Authors: Gintaras Valincius, Vytautas Reipa, M P. Mayhew, V L. Vilker
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=903477

25. New Degenerate Metal-Oxide Electrodes for Nearly Reversible Direct Electron Transfer to the Redox Proteins
Published: 1/1/2002
Authors: Gintaras Valincius, Vytautas Reipa, M P. Mayhew, V L. Vilker
Abstract: Heavily doped cadmium tin oxide (CTO) film electrodes were developed for fast electron exchange with redox proteins. The metal oxide films showed nearly reversible electron transfer for the [2Fe-2S] proteins spinach ferredoxin (Sp fd) and putidaredo ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830264

26. Redox Control of the P450cam Catalytic Cycle: Effects of Y96F Active Site Mutation and Binding of a Non-Natural Substrate
Published: 1/1/2002
Authors: Vytautas Reipa, M P. Mayhew, Marcia J Holden, V L. Vilker
Abstract: Spectroelectrochemical measurements are used to demonstrate that active site mutation and binding of a non-natural substrate to P450cam (CYP101) reduces the shift in the redox potential caused by substrate-binding, and thereby results in slower catal ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830274

27. Electrochemical Properties of Nanocrystalline Cadmium Stannate Films
Published: 8/1/2001
Authors: Gintaras Valincius, Vytautas Reipa, V L. Vilker, John Taylor Woodward IV, Mark D Vaudin
Abstract: Electrochemical properties of the sol-gel nanocrystalline cadmium tin oxide electrodes (CTO) were investigated in 0.2 M potassium chloride buffered at pH 7.4 with phosphate. Films were found to be n-type degenerate semiconductors with charge carrier ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=850463

28. Electrochemical Properties of Nanocrystalline Cadmium Stannate Films
Published: 8/1/2001
Authors: Gintaras Valincius, Vytautas Reipa, V L. Vilker, John Taylor Woodward IV, Mark D Vaudin
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=904228

29. Temperature Dependence of the Formal Reduction Potential of Putidaredoxin
Published: 7/20/2000
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
Abstract: Putidaredoxin (Pdx), a [2Fe-2S] redox protein of size M^dr^ transfers two electrons in two separate steps from the flavin containing putidaredoxin reductase to the heme protein, cytochrome CYP101 in the P450cam catalytic cycle. It has recently come ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830106

30. Improving the Cytochrome P450 Enzyme System for Electrode-Driven Biocatalysis of Styrene Epoxidation
Published: 7/1/2000
Authors: M P. Mayhew, Vytautas Reipa, Marcia J Holden, V L. Vilker
Abstract: Cytochrome P450 enzymes catalyze a vast array of oxidative and reductive biotransformations that are potentially useful for industrial and pharmaceutical syntheses. Factors such as cofactor utilization and slow reaction rates for non-natural substra ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830142



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