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Author: marcia holden
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41. Structure of Archaeal Alanine Dehydrogenase and Relation to Bacterial and Human Proteins
David Travis Gallagher, H G Monbouquette, I Schroeder, Hugh Robinson, Marcia J Holden, N Smith
In the hyperthermophilic archaeon Archaeoglobus fulgidus, gene AF1665 encodes an L-ala dehydrogenase (AlaDH, EC 22.214.171.124) but has been annotated as an ornithine cyclodeaminase (OCD) based on homology with the mu crystalline/ornithine cyclodeaminase pr ...
42. Towards Metrological Traceability for DNA Fragment Ratios in GM Quantitation. Part A The Effect of DNA Extraction Methods on the Quantitative Determination of Bt176 Corn by Real-Time PCR
Ph Corbisier, W Boothaerts, S Giorio, Heinz Schimmel, M J Burns, A Baoutina, K R Emslie, S Furui, Y Kurosawa, Marcia J Holden, H H Kim, Y M Lee, M Kawaharasake, D. W. M. Sin, J. Wang
An international CCQM-P60 pilot study involving 8 national metrological institutes was organized to investigate if the quantification of genetically modified (GM) corn powder by real-time PCR was affected by the DNA extraction methods applied. Four c ...
43. Unclenching Chorismate Lynse: Effects of the C90A Active Site Mutation and of Binding the Inhibitor Vanillate
David Travis Gallagher, N Smith, M P. Mayhew, Marcia J Holden
Chorismate Lyase from E. coli is strongly product inhibited retaining the product 4-hydroxybenzoate internally by extensive hydrogen bonding.The biological rationale for this unusual retention, and the mechanism of release, may involve pathway regula ...