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You searched on: Author: marcia holden

Displaying records 41 to 44.
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41. Interaction Between Putidaredoxin and Its Redox Partner, Putidaredoxin Reductase
Published: Date unknown
Authors: Marcia J Holden, M P. Mayhew, V L. Vilker
Abstract: Using site-directed mutagenesis we altered surface residues on Putidaredoxin to investigate which side-chain residues might be involved in the binding of Putidaredoxin to its redox partner, Putidaredoxin reductase. We focused on the charged residues ...

42. Structure of Archaeal Alanine Dehydrogenase and Relation to Bacterial and Human Proteins
Published: Date unknown
Authors: David Travis Gallagher, H G Monbouquette, I Schroeder, Hugh Robinson, Marcia J Holden, N Smith
Abstract: In the hyperthermophilic archaeon Archaeoglobus fulgidus, gene AF1665 encodes an L-ala dehydrogenase (AlaDH, EC but has been annotated as an ornithine cyclodeaminase (OCD) based on homology with the mu crystalline/ornithine cyclodeaminase pr ...

43. Towards Metrological Traceability for DNA Fragment Ratios in GM Quantitation. Part A The Effect of DNA Extraction Methods on the Quantitative Determination of Bt176 Corn by Real-Time PCR
Published: Date unknown
Authors: Ph Corbisier, W Boothaerts, S Giorio, Heinz Schimmel, M J Burns, A Baoutina, K R Emslie, S Furui, Y Kurosawa, Marcia J Holden, H H Kim, Y M Lee, M Kawaharasake, D. W. M. Sin, J. Wang
Abstract: An international CCQM-P60 pilot study involving 8 national metrological institutes was organized to investigate if the quantification of genetically modified (GM) corn powder by real-time PCR was affected by the DNA extraction methods applied. Four c ...

44. Unclenching Chorismate Lynse: Effects of the C90A Active Site Mutation and of Binding the Inhibitor Vanillate
Published: Date unknown
Authors: David Travis Gallagher, N Smith, M P. Mayhew, Marcia J Holden
Abstract: Chorismate Lyase from E. coli is strongly product inhibited retaining the product 4-hydroxybenzoate internally by extensive hydrogen bonding.The biological rationale for this unusual retention, and the mechanism of release, may involve pathway regula ...

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