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Author: marcia holden

Displaying records 31 to 40 of 43 records.
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31. Crystallization and 1.1 Angstrom Diffraction of Chorismate Lyase From Escherichia Coli
Published: 2/1/2000
Authors: C Stover, M P. Mayhew, Marcia J Holden, A Howard, David Travis Gallagher
Abstract: Clorismate pathway enzymes are important as producers of nonnucleotide aromatic compounds. The enzyme chorismate layase from Escherichia coli has been crystallized in four distinct forms, three of which have been characterized by x-ray diffraction. ...

32. Challenges in Capturing Oxygenase Activity In Vitro
Published: 11/1/1999
Authors: V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J Holden
Abstract: Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large clas ...

33. Comparison of Backbone Dynamics of Oxidized and Reduced Putidaredoxin by ^u15^N NMR Relaxation Measurements
Published: 8/3/1999
Authors: Fahriye Nese Sari, Marcia J Holden, M P. Mayhew, V L. Vilker, B Coxon
Abstract: The backbone dynamics of uniformly ^u15^N-labeled reduced and oxidized putidaredoxin (Pdx) have been studied by 2D ^u15^N NMR relaxation measurements. ^u15^N T^d1^ and T^d2^ values and ^u1^H-^u15^N NOE s have been measured for the diamagnetic region ...

34. A Thermodynamic Investigation of Some Reactions Involving Prephenic Acid
Published: 2/1/1999
Authors: N Kishore, Marcia J Holden, Yadu D. Tewari, Robert Nathan Goldberg
Abstract: Calorimetric enthalpies of reaction have been measured for the following enzyme-catalyzed reactions at the temperature 298.15 Kprephenate(aq) = phenylpyruvate(aq) + H^d2^0(l) + carbon dioxide(aq), prephenate(aq) + NAD^dox^(aq) = 4-hydroxyphenylpyruva ...

35. A Thermodynamic and Quantum Chemical Study of the Conversion of Chorismate to (Pyruvate + 4-Hydroxybenzoate)
Published: 10/22/1998
Authors: Yadu D. Tewari, J Chen, Marcia J Holden, K. N. Houk, Robert Nathan Goldberg
Abstract: A thermodynamic investigation of the conversion of chorismate^u2-^(aq) to {pyruvate^u-^(aq) + 4-hydroxybenzoate^u-^(aq)} has been performed by using microcalorimetry and high-performance liquid chromatography. The study used a genetically engineered ...

36. Binding and Electron Transfer Between Putidaredoxin and Cytochrome P450cam (CYP101). Theory and Experiments
Published: 9/9/1998
Authors: A. E. Roitberg, Marcia J Holden, M P. Mayhew, I V Kurnikov, D N Beratan, V L. Vilker
Abstract: A necessary step in understanding biological function in multiple metalloprotein redox systems is the description of protein-protein binding and electron transfer between two distant metal centers. In this communication, we present experimental and ...

37. Exploration of the Structural Environment of the Iron-Sulfur Cluster in Putidaredoxin by Nitrogen-15 NMR Spectroscopy of Selectively Labeled Cysteine Residues
Published: 8/28/1998
Authors: Fahriye Nese Sari, Marcia J Holden, M P. Mayhew, V L. Vilker, B Coxon
Abstract: Putidaredoxin is a di-iron protein whose paramagnetic region is not well characterized by ^u1^H detected NMR. We have studied the structure of this region in greater detail by directly observed ^u15^N NMR of oxidized and reduced putidaredoxin prepar ...

38. Investigation of the Paramagnetic Domain of Putidaredoxin by Nitrogen-15 NMR Spectroscopy
Published: 11/1/1997
Authors: Bruce Coxon, Fahriye Nese Sari, Marcia J Holden, V L. Vilker

39. Probing the Interactions of Putidaredoxin with Redox Partners in Camphor P450 5-monoxygenase by Mutagenesis of Surface Residues
Published: 8/29/1997
Authors: Marcia J Holden, M P. Mayhew, David M Bunk, A. E. Roitberg, V L. Vilker

40. Interaction Between Putidaredoxin and Its Redox Partner, Putidaredoxin Reductase
Published: Date unknown
Authors: Marcia J Holden, M P. Mayhew, V L. Vilker
Abstract: Using site-directed mutagenesis we altered surface residues on Putidaredoxin to investigate which side-chain residues might be involved in the binding of Putidaredoxin to its redox partner, Putidaredoxin reductase. We focused on the charged residues ...

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