NIST logo

Publications Portal

You searched on:
Author: marcia holden

Displaying records 21 to 30 of 44 records.
Resort by: Date / Title


21. Fluorescence Resonance Energy Transfer Between Donor-Acceptor Pair on Two Oligonucleotides Hybridized Adjacently to a DNA Template
Published: 1/1/2003
Authors: Lili Wang, Adolfas Kastytis Gaigalas, J L. Blasic, Marcia J Holden, David Travis Gallagher, R Pires
Abstract: We have used fluorescein as the energy donor and rhodamine as the acceptor to measure the efficiency of fluorescence resonance energy transfer (FRET) in a set of hybridized DNA constructs. The two fluorophores are covalently attached via linkers to t ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830377

22. Structural Alterations of the Heme Environment of Cytochrome P450cam and the Y96F Mutant as Deduced by Resonance Raman Spectroscopy
Published: 1/1/2003
Authors: G Niaura, Vytautas Reipa, M P. Mayhew, Marcia J Holden, V L. Vilker
Abstract: Resonance Raman spectroscopy at 2.5 cm-1 resolution was used to probe differences in wild type and Y96F mutant P450cam (CYP101), both with and without bound camphor or styrene substrates. In the substrate-free state, the spin state equilibrium is sh ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830245

23. Chorismate Lyase: Kinetics and Engineering for Stability
Published: 1/31/2002
Authors: Marcia J Holden, M P. Mayhew, David Travis Gallagher, V L. Vilker
Abstract: By removing the enolpyruvyl group from chorismate, chorismate lyase (CL) produces p-hydroxybenzoate (p-HB) for the ubiquinone biosynthetic pathway. We have analyzed CL by several spectroscopic and chemical techniques and measured its kinetic (k^dcat^ ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830209

24. Benzocycloarene Hydroxylation by P450 Biocatalysis
Published: 1/1/2002
Authors: M P. Mayhew, A. E. Roitberg, Yadu D. Tewari, Marcia J Holden, David J Vanderah, V L. Vilker
Abstract: Experimental and theorectical studies of the hydroxylation of a family of benzocycloarene compounds (benzocyclobutene, benzocyclopentene (indan), benzocyclohexene (tetralin), and benzocycloheptene) by wild type P450cam and mutant Y96F P450cam were pe ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830255

25. Redox Control of the P450cam Catalytic Cycle: Effects of Y96F Active Site Mutation and Binding of a Non-Natural Substrate
Published: 1/1/2002
Authors: Vytautas Reipa, M P. Mayhew, Marcia J Holden, V L. Vilker
Abstract: Spectroelectrochemical measurements are used to demonstrate that active site mutation and binding of a non-natural substrate to P450cam (CYP101) reduces the shift in the redox potential caused by substrate-binding, and thereby results in slower catal ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830274

26. The Crystal Structure of Chorismate Lyase Shows a New Fold and a Tightly Retained Product
Published: 8/15/2001
Authors: David Travis Gallagher, M P. Mayhew, Marcia J Holden, A J Howard, K J Kim, V L. Vilker
Abstract: The enzyme chorismate lyase (CL) catalyzes the removal of pyruvate from chorismate to produce 4-hydroxy benzoate (4HB) for the ubiquinone pathway. In Escherichia coli, CL is monomeric with 164 residues; we have determined the structure of the CL pro ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830208

27. The Electronic Spectrum of the Prephenate Dianion. An Experimental and Theoretical (MD/QM) Comparison
Published: 8/2/2000
Authors: A. E. Roitberg, S. E. Worthington, Marcia J Holden, M P. Mayhew, Morris Krauss
Abstract: Prephenate is the product of a Claisen rearrangement of chorismate. The enzyme chorismate mutase (CM from B. subtilis) accelerates the reaction by a factor of 10^u6^. The standard method for quantifying prephenate measures the electronic absorption ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830141

28. Temperature Dependence of the Formal Reduction Potential of Putidaredoxin
Published: 7/20/2000
Authors: Vytautas Reipa, Marcia J Holden, M P. Mayhew, V L. Vilker
Abstract: Putidaredoxin (Pdx), a [2Fe-2S] redox protein of size M^dr^ transfers two electrons in two separate steps from the flavin containing putidaredoxin reductase to the heme protein, cytochrome CYP101 in the P450cam catalytic cycle. It has recently come ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830106

29. Improving the Cytochrome P450 Enzyme System for Electrode-Driven Biocatalysis of Styrene Epoxidation
Published: 7/1/2000
Authors: M P. Mayhew, Vytautas Reipa, Marcia J Holden, V L. Vilker
Abstract: Cytochrome P450 enzymes catalyze a vast array of oxidative and reductive biotransformations that are potentially useful for industrial and pharmaceutical syntheses. Factors such as cofactor utilization and slow reaction rates for non-natural substra ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830142

30. Variation in the Formal Reduction Potential of an (2FE-2S) Iron-Sulfur Protein and its Mutants
Published: 5/1/2000
Authors: V L. Vilker, Vytautas Reipa, Marcia J Holden, M P. Mayhew
Abstract: We have been pursuing the detailed pathway by which cytochrome P450cam (CYP101) transfers electrons through a three diffusible protein subunit system, and how this system might be engineered to eliminate nonessential subunits and the costly NADH cofa ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830171



Search NIST-wide:


(Search abstract and keywords)


Last Name:
First Name:







Special Publications:

Looking for a NIST Special Publication (NIST SP Series)? Place the series number and dash in the report number field (Example: 800-) and begin your search.

  • SP 250-XX: Calibration Services
  • SP 260-XX: Standard Reference Materials
  • SP 300-XX: Precision Measurement and Calibration
  • SP 400-XX: Semiconductor Measurement Technology
  • SP 480-XX: Law Enforcement Technology
  • SP 500-XX: Computer Systems Technology
  • SP 700-XX: Industrial Measurement Series
  • SP 800-XX: Computer Security Series
  • SP 823-XX: Integrated Services Digital Network Series