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Author: michael halter
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11. Overlap-Based Cell Tracker
NIST Interagency/Internal Report (NISTIR)
Joe Chalfoun, Antonio Cardone, Alden A Dima, Michael W Halter, Daniel P Allen
In order to facilitate the extraction of quantitative data from live cell image sets, automated image analysis methods are needed. This paper presents an introduction to the general principle of an overlap cell tracking software developed by NIST. Th ...
12. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
13. Cell volume distributions reveal cell growth rates and division times
Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
14. Surface plasmon resonance imaging of cells and surface-associated fibronectin
Alexander W Peterson, Michael W Halter, Alessandro Tona, Kiran Bhadriraju, Anne L Plant
A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for ...