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Author: john elliott
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1. A Human Inspired Local Ratio-Based Algorithm for Edge Detection in Fluorescent Cell Images
Published: 11/25/2010
Authors: Adele P Peskin, Joe Chalfoun, Alden A Dima, John T Elliott, James J Filliben
Abstract: We have developed a new semi-automated method for segmenting images of biological cells seeded at low density on tissue culture substrates, which we use to improve the generation of reference data for the evaluation of automated segmentation algor ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906723

2. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

3. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901347

4. An Electrochemical Biosensor Sensitive to Structure Changes in an Absorbed Phospholipid Layer: Development of a Phospholipase C Biosensor
Published: 2/1/2000
Author: John T Elliott
Abstract: We have fabricated an electrochemical biosensor for detecting structure changes in a phospholipid layer absorbed onto a mixed thioalkyl self-assembled monolayer (SAM). Mixed SAMs containing alkane terminated thiaethyleneoxide derivativesand b-mercapt ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830152

5. Biocompatibility of Sorbitol-Containing Polyesters. Part I: Synthesis, Surface Analysis and Cell Response in vitro
Published: 1/1/2004
Authors: Ying Mei, A Kumar, W Gao, R Gross, S B Kennedy, N. R. Washburn, Eric J. Amis, John T Elliott
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=853927

6. Biological Cell Feature Identification by a Modified Watershed-Merging Algorithm
Series: NIST Interagency/Internal Report (NISTIR)
Published: 11/24/2010
Authors: David E. Gilsinn, Kiran Bhadriraju, John T Elliott
Abstract: Biological cells are composed of many subsystems and organelles. The subsystem called the cytoskeleton is composed of long rod-shaped structures. They give the cell form and help attach the cell to the substrate and neighbors. One of the filaments is ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906615

7. Biomimetic Membranes on Metal Supports
Published: 5/1/2003
Authors: John T Elliott, Curtis W Meuse, Vitalii Ivanovich Silin, Susan T Krueger, John Taylor Woodward IV, T Petralli-Mallow, Anne L Plant
Abstract: Biological membranes are complex and dynamic structures. The biological functions associated with membranes involve a number of different molecular species, and theories of how the molecular species are organized are still evolving. The fluid mosa ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830376

8. Cell volume distributions reveal cell growth rates and division times
Published: 3/7/2009
Authors: Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
Abstract: A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832284

9. Comparison of Reagents for Shape Analysis of Fixed Cells by Automated Fluorescence Microscopy
Published: 4/1/2003
Authors: John T Elliott, Alessandro Tona, Anne L Plant
Abstract: Background: Cell size and shape have been implicated as potentiators intracellular signaling events and as indicator of abnormal cell behavior. Automated microscopy and image analysis can provide quantitative information about the size and shape of ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830369

10. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Published: 6/14/2011
Authors: Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Abstract: Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905998



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