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1. A Human Inspired Local Ratio-Based Algorithm for Edge Detection in Fluorescent Cell Images
Adele P Peskin, Joe Chalfoun, Alden A Dima, John T Elliott, James J Filliben
We have developed a new semi-automated method for segmenting
images of biological cells seeded at low density on tissue culture
substrates, which we use to improve the generation of reference data for
the evaluation of automated segmentation algor ...
2. A New Measure in Cell Image Segmentation Data Analysis
NIST Interagency/Internal Report (NISTIR)
Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
3. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
4. An Electrochemical Biosensor Sensitive to Structure Changes in an Absorbed Phospholipid Layer: Development of a Phospholipase C Biosensor
John T Elliott
We have fabricated an electrochemical biosensor for detecting structure changes in a phospholipid layer absorbed onto a mixed thioalkyl self-assembled monolayer (SAM). Mixed SAMs containing alkane terminated thiaethyleneoxide derivativesand b-mercapt ...
5. Biocompatibility of Sorbitol-Containing Polyesters. Part I: Synthesis, Surface Analysis and Cell Response in vitro
Ying Mei, A Kumar, W Gao, R Gross, S B Kennedy, N. R. Washburn, Eric J. Amis, John T Elliott
6. Biological Cell Feature Identification by a Modified Watershed-Merging Algorithm
NIST Interagency/Internal Report (NISTIR)
David E. Gilsinn, Kiran Bhadriraju, John T Elliott
Biological cells are composed of many subsystems and organelles. The subsystem called the cytoskeleton is composed of long rod-shaped structures. They give the cell form and help attach the cell to the substrate and neighbors. One of the filaments is ...
7. Biomimetic Membranes on Metal Supports
John T Elliott, Curtis W Meuse, Vitalii Ivanovich Silin, Susan Krueger, John Taylor Woodward IV, T Petralli-Mallow, Anne L Plant
Biological membranes are complex and dynamic structures. The biological functions associated with membranes involve a number of different molecular species, and theories of how the molecular species are organized are still evolving. The fluid mosa ...
8. Cell volume distributions reveal cell growth rates and division times
Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
9. Comparison of Reagents for Shape Analysis of Fixed Cells by Automated Fluorescence Microscopy
John T Elliott, Alessandro Tona, Anne L Plant
Background: Cell size and shape have been implicated as potentiators intracellular signaling events and as indicator of abnormal cell behavior. Automated microscopy and image analysis can provide quantitative information about the size and shape of ...
10. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...