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You searched on: Author: john elliott

Displaying records 11 to 20 of 48 records.
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11. A New Measure in Cell Image Segmentation Data Analysis
Series: NIST Interagency/Internal Report (NISTIR)
Report Number: 7871
Published: 7/24/2012
Authors: Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Abstract: Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=911671

12. Evaluation of Segmentation Algorithms on Cell Populations Using CDF Curves
Published: 2/24/2012
Authors: Robert Charles Hagwood, Javier Bernal, Michael W Halter, John T Elliott
Abstract: Cell segmentation is a critical step in the analysis pipeline for most imaging cytometry experiments and the segmentation algorithm can effect the quantitative data derived from image analysis. Methods to evaluate segmentation algorithms are importan ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907743

13. New Concepts for Building Vocabulary for Cell Image Ontologies
Published: 12/21/2011
Authors: Anne L Plant, John T Elliott, Talapady N Bhat
Abstract: Background: We present an approach to cell image databasing that is compatible with searching across a global federation of independent image databases. The variety of biological experiments and data that practitioners would like to access and share ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907774

14. Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity
Published: 12/1/2011
Authors: Justin M Zook, Robert I. MacCuspie, Laurie E Locascio, Michael W Halter, John T Elliott
Abstract: Methods to disperse nanoparticles reproducibly in aqueous solutions compatible with cell-based assays are critical for studying cytotoxicity. Here, we disperse gold and silver nanoparticles in cell culture media, letting them agglomerate to a variet ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906154

15. Estimation and Uncertainty analysis of Dose Response in an inter-laboratory experiment
Published: 11/13/2011
Authors: Blaza Toman, Elijah J Petersen, John T Elliott
Abstract: An inter-laboratory experiment for the evaluation of toxic effects of NH2-polystyrene nanoparticles on living cells was performed with five participating laboratories. Previously published results in the field of nano toxicology have been contradicto ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=919392

16. Hybrid Cell Adhesive Material for Instant Dielectrophoretic Cell Trapping and Long Term Cell Function Assessment
Published: 8/16/2011
Authors: Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
Abstract: We present the development and evaluation of an engineered hybrid cell adhesive surface for instant dielectrophoretic trapping and patterning of cells in a microfluidic device. This work demonstrates that a hybrid cell adhesive material (hCAM) prepar ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906647

17. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Published: 6/14/2011
Authors: Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Abstract: Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905998

18. Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay
Published: 5/15/2011
Authors: Gregory Alan Cooksey, John T Elliott, Anne L Plant
Abstract: Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content cell-based assays. However, maximizing the value of these assays for drug discovery, screening or toxicity evaluation, for example, will require va ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907416

19. Predicting Segmentation Accuracy for Biological Cell Images
Published: 12/15/2010
Authors: Adele P Peskin, Alden A Dima, Joe Chalfoun, John T Elliott
Abstract: We have performed segmentation procedures on a large number of images from two mammalian cell lines that were seeded at low density, in order to study trends in the segmentation results and make predictions about cellular features that affect segm ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906721

20. A Human Inspired Local Ratio-Based Algorithm for Edge Detection in Fluorescent Cell Images
Published: 11/25/2010
Authors: Adele P Peskin, Joe Chalfoun, Alden A Dima, John T Elliott, James J Filliben
Abstract: We have developed a new semi-automated method for segmenting images of biological cells seeded at low density on tissue culture substrates, which we use to improve the generation of reference data for the evaluation of automated segmentation algor ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906723



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