You searched on: Author: john elliott
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11. A New Measure in Cell Image Segmentation Data Analysis
NIST Interagency/Internal Report (NISTIR)
Jin Chu Wu, Michael W Halter, Raghu N Kacker, John T Elliott
Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells trea ...
12. Evaluation of Segmentation Algorithms on Cell Populations Using CDF Curves
Robert Charles Hagwood, Javier Bernal, Michael W Halter, John T Elliott
Cell segmentation is a critical step in the analysis pipeline for most imaging cytometry experiments and the segmentation algorithm can effect the quantitative data derived from image analysis. Methods to evaluate segmentation algorithms are importan ...
13. New Concepts for Building Vocabulary for Cell Image Ontologies
Anne L Plant, John T Elliott, Talapady N Bhat
Background: We present an approach to cell image databasing that is compatible with searching across a global federation of independent image databases. The variety of biological experiments and data that practitioners would like to access and share ...
14. Stable nanoparticle aggregates/agglomerates of different sizes and the effect of their size on hemolytic cytotoxicity
Justin M Zook, Robert I. MacCuspie, Laurie E Locascio, Michael W Halter, John T Elliott
Methods to disperse nanoparticles reproducibly in aqueous solutions compatible with cell-based assays are critical for studying cytotoxicity. Here, we disperse gold and silver nanoparticles in cell culture media, letting them agglomerate to a variet ...
15. Estimation and Uncertainty analysis of Dose Response in an inter-laboratory experiment
Blaza Toman, Elijah J Petersen, John T Elliott
An inter-laboratory experiment for the evaluation of toxic effects of NH2-polystyrene nanoparticles on living cells was performed with five participating laboratories. Previously published results in the field of nano toxicology have been contradicto ...
16. Hybrid Cell Adhesive Material for Instant Dielectrophoretic Cell Trapping and Long Term Cell Function Assessment
Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
We present the development and evaluation of an engineered hybrid cell adhesive surface for instant dielectrophoretic trapping and patterning of cells in a microfluidic device. This work demonstrates that a hybrid cell adhesive material (hCAM) prepar ...
17. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...
18. Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay
Gregory Alan Cooksey, John T Elliott, Anne L Plant
Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content
cell-based assays. However, maximizing the value of these assays for drug discovery, screening or
toxicity evaluation, for example, will require va ...
19. Predicting Segmentation Accuracy for Biological Cell Images
Adele P Peskin, Alden A Dima, Joe Chalfoun, John T Elliott
We have performed segmentation procedures on a large number
of images from two mammalian cell lines that were seeded at low
density, in order to study trends in the segmentation results and make
predictions about cellular features that affect segm ...
20. A Human Inspired Local Ratio-Based Algorithm for Edge Detection in Fluorescent Cell Images
Adele P Peskin, Joe Chalfoun, Alden A Dima, John T Elliott, James J Filliben
We have developed a new semi-automated method for segmenting
images of biological cells seeded at low density on tissue culture
substrates, which we use to improve the generation of reference data for
the evaluation of automated segmentation algor ...