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Author: john elliott

Displaying records 11 to 20 of 41 records.
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11. Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay
Published: 5/15/2011
Authors: Gregory A Cooksey, John T Elliott, Anne L Plant
Abstract: Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content cell-based assays. However, maximizing the value of these assays for drug discovery, screening or toxicity evaluation, for example, will require va ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907416

12. Predicting Segmentation Accuracy for Biological Cell Images
Published: 12/15/2010
Authors: Adele P Peskin, Alden A Dima, Joe Chalfoun, John T Elliott
Abstract: We have performed segmentation procedures on a large number of images from two mammalian cell lines that were seeded at low density, in order to study trends in the segmentation results and make predictions about cellular features that affect segm ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906721

13. A Human Inspired Local Ratio-Based Algorithm for Edge Detection in Fluorescent Cell Images
Published: 11/25/2010
Authors: Adele P Peskin, Joe Chalfoun, Alden A Dima, John T Elliott, James J Filliben
Abstract: We have developed a new semi-automated method for segmenting images of biological cells seeded at low density on tissue culture substrates, which we use to improve the generation of reference data for the evaluation of automated segmentation algor ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906723

14. Biological Cell Feature Identification by a Modified Watershed-Merging Algorithm
Series: NIST Interagency/Internal Report (NISTIR)
Published: 11/24/2010
Authors: David E. Gilsinn, Kiran Bhadriraju, John T Elliott
Abstract: Biological cells are composed of many subsystems and organelles. The subsystem called the cytoskeleton is composed of long rod-shaped structures. They give the cell form and help attach the cell to the substrate and neighbors. One of the filaments is ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906615

15. Instantaneous Trapping and Long Term Cell Survival Under Dielectrophoretic Conditions Using a Hybrid Cell Adhesive Surface
Published: 10/3/2010
Authors: Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
Abstract: A dielectrophoresis (DEP) microfluidic device that allows instantaneous trapping of cells for rapid return to cell growth conditions (within 5 minutes) and long-term cell experiments (cell proliferation, induction and differentiation) of embryonic P1 ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906185

16. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901347

17. Cell volume distributions reveal cell growth rates and division times
Published: 3/7/2009
Authors: Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
Abstract: A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832284

18. Determination of Protein Aggregation with Differential Mobility Analysis: Application to IgG Antibody
Published: 12/15/2008
Authors: Leonard F. Pease III, John T Elliott, De-Hao D. Tsai, Michael Russel Zachariah, Michael J Tarlov
Abstract: Here we describe the use of electrospray differential mobility analysis (ES-DMA), also known as gas-phase electrophoretic mobility molecular analysis (GEMMA), as a method for measuring low-order aggregates of proteins in solution. We demonstrate pro ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832275

19. The Stiffness of Collagen Fibrils Influences Vascular Smooth Muscle Cell Phenotype
Published: 3/1/2007
Authors: Dennis P. McDaniel, Gordon Allan Shaw, John T Elliott, Kiran Bhadriraju, Curtis W Meuse, Koo-Hyun Chung, Anne L Plant
Abstract: Cells receive signals from the extracellular matrix through receptor-dependent interactions, but they are also influenced by the mechanical properties of the matrix. While bulk properties of substrates have been shown to effect cell behavior, we sho ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=830514

20. The effect of surface chemistry on the formation of thin films of native fibrillar collagen
Published: 2/1/2007
Authors: John T Elliott, John Taylor Woodward IV, A. Umarji, Y. Mei, Alessandro Tona
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=908012



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