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Author: john elliott

Displaying records 11 to 20 of 43 records.
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11. Hybrid Cell Adhesive Material for Instant Dielectrophoretic Cell Trapping and Long Term Cell Function Assessment
Published: 8/16/2011
Authors: Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
Abstract: We present the development and evaluation of an engineered hybrid cell adhesive surface for instant dielectrophoretic trapping and patterning of cells in a microfluidic device. This work demonstrates that a hybrid cell adhesive material (hCAM) prepar ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906647

12. Comparison of segmentation algorithms for fluorescence microscopy images of cells
Published: 6/14/2011
Authors: Alden A Dima, John T Elliott, James J Filliben, Michael W Halter, Adele P Peskin, Javier Bernal, Marcin Kociolek, Mary C Brady, Hai C. Tang, Anne L Plant
Abstract: Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=905998

13. Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay
Published: 5/15/2011
Authors: Gregory A Cooksey, John T Elliott, Anne L Plant
Abstract: Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content cell-based assays. However, maximizing the value of these assays for drug discovery, screening or toxicity evaluation, for example, will require va ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=907416

14. Predicting Segmentation Accuracy for Biological Cell Images
Published: 12/15/2010
Authors: Adele P Peskin, Alden A Dima, Joe Chalfoun, John T Elliott
Abstract: We have performed segmentation procedures on a large number of images from two mammalian cell lines that were seeded at low density, in order to study trends in the segmentation results and make predictions about cellular features that affect segm ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906721

15. A Human Inspired Local Ratio-Based Algorithm for Edge Detection in Fluorescent Cell Images
Published: 11/25/2010
Authors: Adele P Peskin, Joe Chalfoun, Alden A Dima, John T Elliott, James J Filliben
Abstract: We have developed a new semi-automated method for segmenting images of biological cells seeded at low density on tissue culture substrates, which we use to improve the generation of reference data for the evaluation of automated segmentation algor ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906723

16. Biological Cell Feature Identification by a Modified Watershed-Merging Algorithm
Series: NIST Interagency/Internal Report (NISTIR)
Published: 11/24/2010
Authors: David E. Gilsinn, Kiran Bhadriraju, John T Elliott
Abstract: Biological cells are composed of many subsystems and organelles. The subsystem called the cytoskeleton is composed of long rod-shaped structures. They give the cell form and help attach the cell to the substrate and neighbors. One of the filaments is ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906615

17. Instantaneous Trapping and Long Term Cell Survival Under Dielectrophoretic Conditions Using a Hybrid Cell Adhesive Surface
Published: 10/3/2010
Authors: Darwin R Reyes-Hernandez, Jennifer S. Hong, John T Elliott, Michael Gaitan
Abstract: A dielectrophoresis (DEP) microfluidic device that allows instantaneous trapping of cells for rapid return to cell growth conditions (within 5 minutes) and long-term cell experiments (cell proliferation, induction and differentiation) of embryonic P1 ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=906185

18. A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
Published: 8/1/2009
Authors: Michael W Halter, Jamie L Almeida, Alessandro Tona, Kenneth D Cole, Anne L Plant, John T Elliott
Abstract: Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that prov ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=901347

19. Cell volume distributions reveal cell growth rates and division times
Published: 3/7/2009
Authors: Michael W Halter, John T Elliott, Joseph Bunton Hubbard, Alessandro Tona, Anne L Plant
Abstract: A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832284

20. Determination of Protein Aggregation with Differential Mobility Analysis: Application to IgG Antibody
Published: 12/15/2008
Authors: Leonard F. Pease III, John T Elliott, De-Hao D. Tsai, Michael Russel Zachariah, Michael J Tarlov
Abstract: Here we describe the use of electrospray differential mobility analysis (ES-DMA), also known as gas-phase electrophoretic mobility molecular analysis (GEMMA), as a method for measuring low-order aggregates of proteins in solution. We demonstrate pro ...
http://www.nist.gov/manuscript-publication-search.cfm?pub_id=832275



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