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You searched on: Author: gregory cooksey Sorted by: date

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1. Optofluidic Temperature and Pressure Measurements with Fiber Bragg Gratings Embedded in Microfluidic Devices
Published: 5/22/2016
Authors: Gregory Alan Cooksey, Zeeshan Ahmed
Abstract: The integration of photonic sensors into microfluidic devices provides opportunities for dynamic measurement of chemical and physical properties of fluids in very small volumes. We previously reported on the use of commercially available Fiber Br ...

2. Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass
Published: 8/11/2014
Authors: Michael W Halter, Elianna Bier, Paul C DeRose, Gregory Alan Cooksey, Steven J Choquette, Anne L Plant, John T Elliott
Abstract: Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist f ...

3. Pneumatic valves in folded 2-D and 3-D fluidic devices made from plastic sheets and tapes
Published: 4/1/2014
Authors: Gregory Alan Cooksey, Francisco Javier Atencia
Abstract: We present a rapid prototyping technique that expands elastomeric valving capabilities to devices made from thin materials such as plastic films and tapes. A key characteristic of this technology is that devices are thin (typically less than 0.5 ...

4. Measurement and validation of cell-based assays with microfluidics at the National Institute of Standards and Technology (NIST)
Published: 8/1/2012
Authors: Gregory Alan Cooksey, Francisco Javier Atencia, Samuel P Forry

5. A robust diffusion-based gradient generator for dynamic cell assays.
Published: 1/1/2012
Authors: Francisco Javier Atencia, Gregory Alan Cooksey, Laurie E Locascio
Abstract: This manuscript describes a new method to generate purely diffusive chemical gradients that can be modified in time. The device is simple in its design and easy to use, which makes it amenable to study biological processes that involve static or dy ...

6. Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay
Published: 5/15/2011
Authors: Gregory Alan Cooksey, John T Elliott, Anne L Plant
Abstract: Numerous opportunities exist to apply microfluidic technology to high-throughput and high-content cell-based assays. However, maximizing the value of these assays for drug discovery, screening or toxicity evaluation, for example, will require va ...

7. Chips and Tips: Modified luer adapters with small dead volume that fit small tubing
Published: 6/28/2010
Authors: Gregory Alan Cooksey, glynnis Mattheisen
Abstract: Luer adapters are convenient tools to rapidly and reversibly connect tubing from fluid reservoirs to microfluidic devices. One problem associated with the use of these adapters is that they trap considerable dead volume (60 ul, in the case of the ada ...

8. Magnetic connectors for microfluidic applications
Published: 1/1/2010
Authors: Francisco Javier Atencia, Gregory Alan Cooksey, Andreas Jahn, Justin M Zook, Wyatt N Vreeland, Laurie E Locascio
Abstract: We present a new type of connector based on a simple idea: using a ring magnet on one side of the microfluidic chip and a disc magnet on the other side to provide the interfacial force necessary to provide a sealed connection between tubing or needle ...

9. Controlling the Flow Rate of Many Fluids With Floating Vials In a Pressurizeable Container
Published: 11/1/2009
Authors: Gregory Alan Cooksey, Anne L Plant
Abstract: We report a simple strategy to control the pressure-driven flow rates from fluid reservoirs to a microchip in an efficient and compact fashion. We demonstrate that using a floating reservoir, less than 1 percent change in flow rate per ml of deliver ...

10. A vacuum manifold for rapid world-to-chip connectivity of complex PDMS microdevices
Published: 1/1/2009
Authors: Gregory Alan Cooksey, Anne L Plant, Francisco Javier Atencia
Abstract: The lack of simple interfaces for microfluidic devices with a large number of inlets significantly limits production and utilization of these devices. In this paper, we describe the fabrication of a reusable manifold that provides rapid world-to-chi ...

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