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Author: kenneth cole

Displaying records 31 to 40 of 48 records.
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31. DNA Electrophoresis in Gellan Gels. The Effect of Electroosmosis and Polymer Additives
Published: 3/7/2002
Authors: M. Markstrom, Kenneth D Cole, B. Akerman
Abstract: The polysacharide gellan forms hydrogels if a divalent ion such as calcium is added at millimolar concentrations. The gel can be reversed to solution by adding EDTA, which makes it a promising candidate for preparative electrophoretic separation of b ...

32. Separation of Large Circular DNA by Electrophoresis in Agarose Gels
Published: 1/1/2002
Authors: Kenneth D Cole, C M Tellez
Abstract: The electrophoresis of circular DNA, ranging in size from 4.4 kilobase pairs (kbp) to 220 kbp, was studied in agarose gels. Bacterial artificial chromosome (BAC) DNA was used as a source of large supercoiled and open circular (relaxed) forms. the o ...

33. Controlling Electrophoretic Trapping of Circular DNA by Addition of Starch Preparations to Agarose Gels
Published: 7/1/2001
Authors: Kenneth D Cole, C M. Tellez-Rodriguez, R Nguyen

34. Enhanced Capacity for Electrophoretic Capture of Plasmid DNA by Agarase Treatment of Agarose Gels
Published: 12/1/2000
Authors: Kenneth D Cole, B. Akerman
Abstract: Agarase was used investigate the effect of increasing the number of polymer ends on the electrophoretic trapping of circular DNA in agarose gels. The electric field strength required to trap circular DNA was found to be the same in control and treat ...

35. Laboratory Database to Manage Electrophoresis and Chromatography Separations and the Associated Samples
Published: 12/1/2000
Author: Kenneth D Cole
Abstract: A database was developed to store, organize, and retrieve the data associated with electrophoresis and chromatography separations. It allows laboratories to store extensive data on saparation techniques (analytical and preparative). The data for gel ...

36. Enachanced Capacity for Electrophoretic Capture of Plasmid DNA by Agarase Treatment of Agarase Gels.
Published: 7/1/2000
Author: Kenneth D Cole

37. Enhanced Capacity for Electrophoresis and Chromatography Separations and the Associated Samples.
Published: 7/1/2000
Author: Kenneth D Cole

38. Preparative Electrochromatography of Proteins in Various Types of Porous Media
Published: 3/1/2000
Authors: C M Tellez, Kenneth D Cole
Abstract: The performance of commercial and enzymatically-modified size-exclusion (SE) gels in electrochromatography was compared for preparative protein separations. Dextran and agarose-based SE gels were subjected to enzymatic digestion under mild conditions ...

39. Separation of Different Physical Forms of Plasmid DNA Using a Combination of Low Electric Field Strength and Flow in Porous Media: Effect of Different Field Gradients and Porosity of the Media
Published: 3/1/2000
Authors: Kenneth D Cole, C M. Tellez-Rodriguez, R W Blakesley
Abstract: The retention of different physical forms of DNA by an electric field in a chromatography system was studied. We were able to effectively separate the supercoiled and the open circular forms of plasmid DNA using this type of electrochromatography sy ...

40. Modification of the Electrokinetic Properties of Reversible Electrophoresis Gels for the Separation and Preparation of DNA
Published: 9/1/1999
Authors: Kenneth D Cole, R Nguyen, C M. Tellez-Rodriguez
Abstract: The effect of adding linear polymers to a novel reversible electrophoretic media was measured. Reversible gels are formed using the polyanionic carbohydrate polymer, gellan gum. Gellan gum forms strong stable gels in the presence of divalent cation ...

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