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Jeeseong Hwang

Jeeseong Hwang is a research biophysicist in the Physical Measurement Laboratory at the National Institute of Standards and Technology (NIST) of US Department of Commerce. His work at NIST in 1994 began with an award for a National Research Council Postdoctoral Fellowship to study near-field optical scanning probe microscopy of nanoscale biomimetic materials and structures. Before coming to NIST, he was a research scientist in an immunology laboratory at the biology department of the Johns Hopkins University, investigating biophysical aspects of immune response of human cells using super-resolution near-field scanning optical microscopy and other laser-based optical imaging techniques in collaboration with AT&T Bell Laboratories. His Ph. D thesis work at the physics department of Michigan State University was on scanning probe microscopy of nanoscale quantum structures and metal surfaces.

His recent research in the Molecular Applications Project focuses on nanobiophotonics for quantitative biophysics and nanomedicine, involving development and application of the following areas: (1) multimodal molecular imaging techniques; (2) integrated optical analysis platforms for multiplexed bio-assays; (3) bio-inspired assembly of nanoscale building blocks for optical bio-sensors; and (4) quantitative image analysis software for multiplexed dynamic bio-assays.

Recent contributions to professional societies include serving on a steering committee of the IEEE-Nanotechnology Conference in 2010, a program committee of the annual SPIE BiOS convention since 2006, and a ISO scanning probe microscopy subcommittee in 2005-2007. He received a NIST/DoC Silver Medal for “the development of an innovative nanotechnology tool for the highly sensitive and selective optical detections of bacterial pathogens” in 2008. He has authored 53 technical papers, delivered 28 invited talks, and holds two U.S. Patents.

Nano-biophotonics consists of four broad areas: molecular bioimaging; nano-biosensors; multiplexed bioassays; and nanotechnology-based medical practices for diagnosis and therapy. Success in these areas is challenged by the underlying complexity of biological systems. Major levels of complexity and associated technical barriers appear at all levels of biology including molecular, cellular, and systems biology level. To address these challenges, our research strategies are based on the following projects.

Research Projects:

  • Advanced integrated microscopy
    • Time-correlated confocal nano-spectroscopic microscopy of single and clustered nanoprobes
    • Integrated microscopy of photo-excited nanoparticles and related processes
    • Optical tweezers and single molecule microscopy of biomolecular interactions
    • Multimodal optical microscopy of molecular dynamics and cellular processes
    • Hyperspectral microscopy of in vivo simulated cell culture
  • Image analysis software for quantitative and dynamic molecular imaging
    • NIST’s Integrated Colony Enumerator (NICE)
    • Single particle tracking analysis
    • Dynamic imaging of cellular processes
  • Next generation optical bioassays
    • Nano-biosensors of nanoscale building blocks
    • Multiplexed high-throughput biomolecular assays
    • Fusion proteins for dynamical studies of infectious diseases
Jeeseong Hwang


Research Biophysicist
Quantum Electronics and Photonics Division
Sources and Detectors Group

Employment History:

1997-present, NIST, Gaithersburg, MD

1993-1997, The Johns Hopkins University, Baltimore, MD


Ph.D. Condensed Matter Physics, Michigan State University, East Lansing, MI

B.S. Physics, Seoul National University, Seoul, Korea


Phone: 301-975-4580
Fax: 301-975-6991