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Bioscience and Health


Key metrologies/systems: In situ spectroscopic ellipsometry/Self-assembled monolayer resistance to protein/surface binding. Advanced platforms and protocols are being developed towards the realization of robust surface coatings based on thio-tethered oligo(ethylene oxide) to facilitate resistance to protein and other biomolecule binding on surfaces.


Non-specific Protein Adsorption Resistant Films

The development of methodologies to control non-specific adsorption of proteins onto surfaces is needed to address the ever-increasing problem of biofouling. Formation of biofilms on ship hulls, within drainage pipes, or on implantable medical instruments and devices can create detrimental effects in and greatly impair the use of affected items or structures. This research program focuses on the synthesis and characterization of molecules that self-assemble onto surfaces such as Au and form layers that resist or reject non-specific protein adsorption. A compound was developed that nearly completely rejects adsorption of proteins such as fibrinogen and bovine serum albumin at protein concentrations approaching physiological conditions.

Associated Publications:

 “In-Situ Characterization of Oligo(ethylene oxide) Self-Assembled Monolayers Using Spectroscopic Ellipsometry”, M.L. Walker, D.J. Vanderah and K. Rubinson, submitted to Langmuir    

“Self-assembled monolayers of an oligo(ethylene oxide) disulfide and its corresponding thiol assembled from water: Characterization and protein resistance”, D.J. Vanderah, M.L. Walker, M.A. Rocco and K. Rubinson, Langmuir 24, 826 (2008).

Lead Organizational Unit:


Marlon Walker
Email:  marlon.walker@nist.gov
Tel:  301-975-5593