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Dr. Peter M. Vallone

Peter M. Vallone received his Ph.D. in Chemistry from the University of Illinois at Chicago in 1999. In 1999 he was awarded a NRC postdoctoral fellowship that brought him to the Biotechnology Division at National Institute of Standards and Technology (NIST). After completing his postdoctoral work in 2001 Dr. Vallone became a permanent staff scientist at NIST. During the last 14 years Dr. Vallone has worked on developing multiplex assays for the detection of genetic variation, developed methods for the rapid amplification of STR loci, and has been involved in the characterization of nucleic acid-based reference materials. Dr. Vallone has also developed various bioinformatic software tools for the design of nucleic acid based assays (e.g. AutoDimer). Dr. Vallone has published over 40 peer-reviewed articles in the area of DNA thermodynamics and forensic DNA testing. He is currently the leader of the Applied Genetics group at NIST.

2009 International Society of Forensic Genetics, Best Poster Prize Award

2008 Department of Commerce Gold Medal Award.
For research and measurement services in the area of human identity testing to support the criminal justice, legal, and disaster recovery communities.

1999 NRC Postdoctoral Fellowship

Selected Publications
Fondevila, M., Phillips, C., Santos, C., Freire Aradas, A., Vallone, P.M., Butler, J.M., Lareu, M.V., Carracedo, A. (2013) Revision of the SNPforID 34-plex forensic ancestry test: assay enhancements, standard reference sample genotypes and extended population studies. Forensic Sci. Int. Genet. 7: 63-74.  

Oostdik, K., French, J., Yet, D., Smalling, B., Nolde, C., Vallone, P.M., Butts, E.L.R., Hill, C.R., Kline, M.C., Rinta, T., Gerow, A.M., Allen, S.R., Huber, C.K., Teske, J., Krenke, B., Ensenberger, M., Fulmer, P., Sprecher, C. (2013) Developmental validation of the PowerPlex® 18D System, a rapid STR multiplex for analysis of reference samples.Forensic Sci. Int. Genet. 7: 129-135.  

Vallone, P.M. (2012) Capillary electrophoresis of an 11plex mtDNA coding region SNP single base extension assay for discrimination of the most common Caucasian HV1/HV2 mitotype. Chapter 11 in Antonio Alonso (ed.), DNA Electrophoresis Protocols for Forensic Genetics (Methods in Molecular Biology, vol. 830). Humana Press: New York. pp. 159-167.

Butts, E.L.R. and Vallone, P.M. (2012) 3500 Genetic Analyzer: Validation Studies. Forensic News (February 2012). Available at

Fondevila, M., Phillips, C., Santos, C., Pereira, R., Gusmao, L., Carracedo, A., Butler, J.M., Lareu, M.V., Vallone, P.M. (2012) Forensic performance of two insertion-deletion marker assays. Int. J. Legal Med. 126: 725-737.

Corach, D., Lao, O., Bobillo, C., van der Gaag, K., Zuniga, S., Vermeulen, M., van Duijn, K., Goedbloed, M., Vallone, P.M., Parson, W., de Knijff, P., Kayser, M. (2010) Inferring continental ancestry of Argentineans from autosomal, Y-chromosomal and mitochondrial DNA. Ann. Hum. Genet. 74: 65-76.

Lao, O., Vallone, P.M., Coble, M.D., Diegoli, T.M., Irwin, J.A., van der Gaag, K., de Knijff, P., Kayser, M. (2010). Evaluating self-declared ancestry of U.S. Americans using autosomal, Y-chromosomal and mitochondrial DNA. Human Mutation, 31(12):E1875-93.

Hill, C.R., Butler, J.M., Vallone, P.M. (2009) A 26plex autosomal STR assay to aid human identity testing. J. Forensic Sci. 54(5): 1008-1015. -- see also

Kline, M.C., Duewer, D.L., Travis, J.C., Smith, M.V., Redman, J.W., Vallone, P.M., Decker, A.E., Butler, J.M. (2009) Production and certification of NIST Standard Reference Material 2372 Human DNA Quantitation Standard. Anal. Bioanal. Chem. 394: 1183-1192.

Vallone, P.M., Hill, C.R., Butler, J.M. (2008) Demonstration of rapid multiplex PCR amplification involving 16 genetic loci. FSI Genetics 3: 42-45.

Vallone, P.M., Jakupciak, J.P., Coble, M.D. (2007) Forensic application of the Affymetrix human mitochondrial resequencing array. FSI Genetics 1: 196-198.

Butler, J.M., Coble, M.D., Vallone, P.M. (2007) STRs vs SNPs: thoughts on the future of forensic DNA testing. Forensic Science, Medicine and Pathology 3: 200-205.

Vallone, P.M., Decker, A.E. and Butler J.M. (2005) Allele Frequencies for 70 Autosomal SNP Loci with U.S. Caucasian, African American, and Hispanic Samples Forensic Science International 149: 279-86.

Vallone, P.M. and Butler, J.M. (2004) AutoDimer: a screening tool for primer-dimer and hairpin structures. Biotechniques, 37: 226-231.

Vallone, P.M., Just, R.S., Coble, M.D., Butler, J.M., Parsons, T.J. (2004) A multiplex allele-specific primer extension assay for forensically informative SNPs distributed throughout the mitochondrial genome. Int. J. Legal Med., 118: 137-146.

Vallone, P.M. and Butler, J.M. (2004) Y-SNP typing of U.S. African American and Caucasian samples using allele-specific hybridization and primer extension. J. Forensic Sci. 49(4): 723-732.

Butler, J.M., Schoske, R., Vallone, P.M., Kline, M.C., Redd, A.J., and Hammer, M.F. (2002) A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers Forensic Sci Int. 129: 10-24.

Benight, A.S., Pancoska P., Owczarzy, R., Vallone, P.M., Nesetril, J., and Riccelli, P.V. (2001) Calculating sequence-dependent melting stability of duplex DNA oligomers and multiplex sequence analysis by graphs. Methods Enzymol 340:165-92.

Owczarzy, R., Vallone, P.M., Gallo, F.J., Paner, T.M., Lane, M.J., and Benight, A.S. (1997) Predicting sequence-dependent melting stability of short duplex DNA oligomers. Biopolymers 44: 217-239.



Group Leader
Biomolecular Measurement Division
Applied Genetics Group


Chemistry Ph.D. 1999
University of Illinois at Chicago


Phone: 1-301-975-4872
Fax: 1-301-975-8505