Clinical DNA Publications and Presentations

2013 Publications

RJ Haynes, MC Kline, B Toman, C Scott, P Wallace, JM Butler and MJ Holden. "Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA." The Journal of Molecular Diagnostics, Vol. 15, No. 2, March 2013 [pdf]

JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, RJ Haynes, J Hellemans, M Kubista, RD Mueller, T Nolan, MW Pfaffl, GL Shipley, J Vandesompele, CT Wittwer, and SA Bustin. "Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments" Clinical Chemistry (epub) [In Press pdf]

 

2013 Presentations

Ross Haynes, Prasad Reddy, Marcia Holden, Margaret Kline, Pete Vallone. "Future Standard Reference Material for BK Virus: Issues and Update" Clinical Virology Symposium 29 (Daytona Beach, FL) April 28 to May 1, 2012 [Click here for pdf]

Ross Haynes "Clinical Uses of Digital PCR and Measurement Issues," Integrating Digital PCR (Boston MA) April 11-12, 2013 [Click here for pdf]

Ross Haynes "Issues Concerning Extraction Efficiency, Methods, and Direct dPCR," Sample Prep & Target Enrichment (Boston, MA) April 10-11, 2013 [Click here for pdf]

 

2012 Presentations

Ross Haynes "Digital PCR & DNA Quantitation" Forensics@NIST (Gaithersburg, MD) webcast (10:23) available at http://www.nist.gov/oles/forensics/nist-forensics-2011-webcast.cfm

Margaret C. Kline and David L. Duewer. “Metrology for Huntington’s Disease PCR Assays: Repeat Regions to Avoid,” Poster #G19 at Association for Molecular Pathology Annual Meeting on Genomic Medicine, (Long Beach, CA), October 25-27, 2012 [Click Here for .pdf]

Ross J. Haynes, Marcia J. Holden, Margaret C. Kline, and John M. Butler. “Model for Production of Nucleic Acid-Based NIST Standard Reference Materials for Clinical Diagnostics,” Poster #ID62 at Association for Molecular Pathology Annual Meeting on Genomic Medicine, (Long Beach, CA), October 25-27, 2012 [Click Here for .pdf]

Ross Haynes. “Principles of Digital PCR and Measurement Issues: The certification of Cytomegalovirus Standard Reference Material (SRM 2366) as a model for future SRMs” Digital PCR Applications and Advances (San Diego, CA), October 15-16, 2012 [Click Here for .pdf]

Ross Haynes, Marcia Holden, Margaret Kline, and John Butler. Current and Future Viral DNA Standard Reference Materials: Cytomegalovirus as a Model. Poster at 28th Clinical Virology Symposium, (Daytona Beach, FL), April 22-25, 2012 [Click Here for .pdf]

 

2011 Publications

Marcia J. Holden, et. al. "Molecular diagnostics: harmonization through reference materials, documentary standards and proficiency testing." Expert Rev. Mol. Diagn. 11(7), 741–755 (2011) [Click Here for .pdf]

2010 Presentations/Publications

Roberta M. Madej, Jack Davis, Marcia J. Holden, Stan Kwang,
Emmanuel Labourier, and George J. Schneider. "International Standards and Reference Materials for Quantitative Molecular Infectious Disease Testing." J Mol Diagn. 2010 Mar;12(2):133-43. [Click Here for .pdf]

John M. Butler. "Dialogue with NIST: Standard Reference Materials for Molecular Diagnostics," Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

Margaret C. Kline and John M. Butler. "Cautionary Considerations When Exploring Cell Lines as Potential Reference Materials," Poster #OTH05 at Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

Margaret C. Kline, David L. Duewer, and John M. Butler. "Characterizing the electrophoretic mobility of Huntington Disease Alleles 13-77: Are the deviations from the theoretical values intrinsic or internal sizing artifacts?" Poster #G09 at Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

Ross J. Haynes, Marcia J. Holden, Margaret C. Kline, and John M. Butler. "NIST Candidate Standard Reference Material (SRM): Cytomegalovirus DNA," Poster #ID19 at Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

 

2009 Presentations

Ross J. Haynes, Marcia J. Holden, Margaret C. Kline, and John M. Butler. "Cytomegalovirus Standard Reference Material® Development for Nucleic Acid Amplification Technologies," Poster at Next Generation Dx Summit (Washington D.C.) August 10-12, 2009 [Click Here for pdf]

Ross J. Haynes, Marcia J. Holden, Margaret C. Kline, and John M. Butler. "Cytomegalovirus Standard Reference Material Development for Nucleic Acid Amplification Technologies" Poster #ID20 at Association for Molecular Pathology Annual Meeting, (Kissimmee, FL), November 19-22, 2009 [Click Here for pdf]

Margaret C. Kline, Marcia J. Holden, Ross J. Haynes, Amy E. Decker*, Carolyn R. Hill, Janette W. Redman, Peter M. Vallone, and John M. Butler. "Emerging Efforts in Clinical Genetics by the Applied Genetics Group at the National Institute of Standards and Technology," Poster #G47 at Association for Molecular Pathology Annual Meeting, (Kissimmee, FL), November 19-22, 2009 [Click Here for pdf]

Margaret C. Kline, Amy E. Decker*, David L. Duewer and John M. Butler. "A NIST Standard Reference Material for Huntington Disease Alleles," Poster #G48 at Association for Molecular Pathology Annual Meeting, (Kissimmee, FL), November 19-22, 2009 [Click Here for pdf]