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Clinical DNA Publications and Presentations

2015 Presentations
JA BuckPM Vallone, and J Harenza. "Investigation of reference sequence and variant caller effects on single nucleotide polymorphism (SNP) calls made across six BK virus genotypes". NIST Summer High School Internship Program (Gaithersburg, MD). August 4, 2015. [Click here for pdf]

PM Vallone. "Challenges of prioritising reference materials". Standardisation of Genomic Amplification Techniques Meeting. (London, UK). June 26, 2015. [Click here for pdf]

KM Kiesler, PM Vallone. "Residual Host Cell DNA Measurement". Biomanufacturing Program Review (Gaithersburg, MD). June 12, 2015. [Click here for pdf]

J Harenza, H Parikh, X Wen, R Patidar, S Sindiri, J Wei, M Salit, P Meltzer, J Khan, J Zook. "Use of SVClassify to classify pediatric solid tumor variant calls as likely true or false positives". American Association for Cancer Research (Philadelphia, PA). April 20, 2015. [Click here for pdf]

J Harenza. "Genetics and Genomics in Biomedical Research: Academic and Government Experiences." St. Vincent College Biology Department Seminar (Latrobe, PA). March 27, 2015. [Click here for pdf]

PM Vallone. "Digital PCR as a method to quantitate DNA." Technical Workshop on the Standardisation of Serological and PCR Assays for the Detection of Ebolavirus (NIBSC, South Mimms, Potters Bar, Hertfordshire, UK). March 6, 2015. [Click here for pdf]

J Harenza. "An Update on DNA Standards for BK and JC Viruses." Association for Molecular Pathology Reference Materials Forum (Washington, DC). November 11, 2014. [Click here for pdf]

J Harenza. "Preliminary Results from a NIST Clinical DNA Reference Materials Needs Survey." Association for Molecular Pathology Reference Materials Forum (Washington, DC). November 11, 2014. [Click here for pdf]

RJ Haynes. "Update for SRM 2366a Cytomegalovirus DNA." Association for Molecular Pathology Reference Materials Forum (Washington, DC). November 11, 2014. [Click here for pdf]

2014 Presentations

JA Buck, PM Vallone, and J Harenza. "Design and Optimization of Primer and Probe Sets for BK Virus Candidate SRM 2365". NIST Summer High School Internship Program (Gaithersburg, MD). August 5, 2014. [Click here for pdf]

J Harenza, JA Buck, L Cook, and PM Vallone. "An update on a candidate BK virus DNA standard reference material". American Association for Clinical Chemistry (Chicago, Illinois). July 29, 2014. [Click here for pdf]

J Harenza. "An update on DNA viral reference materials at NIST". Advanced Virus Detection Technologies User Interest Group Meeting (teleconference/webinar). June 4, 2014. [Click here for pdf]

J Harenza. "The use of microfluidic and droplet based digital PCR platforms for DNA quantitation". Standardisation of Genomic Amplification Techniques Meeting (Graz, Austria). May 29, 2014. [Click here for pdf]

PM Vallone. "An update on CMV and BKV DNA reference materials". Standardisation of Genomic Amplification Techniques Meeting (Graz, Austria). May 28, 2014. [Click here for pdf]


2013 Publications

RJ Haynes, MC Kline, B Toman, C Scott, P Wallace, JM Butler and MJ Holden. "Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA." The Journal of Molecular Diagnostics, Vol. 15, No. 2, March 2013 [pdf]

J Huggett, CA Foy, V Benes, K Emslie, JA Garson, RJ Haynes, J Hellemans, M Kubista, RD Mueller, T Nolan, MW Pfaffl, GL Shipley, J Vandesompele, CT Wittwer, and SA Bustin. "Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments" Clinical Chemistry 59 (6), 892-9022013. [pdf]

 

2013 Presentations

RJ Haynes, P Reddy, MJ Holden, MC Kline, PM Vallone. "Future Standard Reference Material for BK Virus: Issues and Update" Clinical Virology Symposium 29 (Daytona Beach, FL) April 28 to May 1, 2012 [Click here for pdf]

RJ Haynes. "Clinical Uses of Digital PCR and Measurement Issues," Integrating Digital PCR (Boston MA) April 11-12, 2013 [Click here for pdf]

RJ Haynes. "Issues Concerning Extraction Efficiency, Methods, and Direct dPCR," Sample Prep & Target Enrichment (Boston, MA) April 10-11, 2013 [Click here for pdf]

 

2012 Presentations

RJ Haynes. "Digital PCR & DNA Quantitation" Forensics@NIST (Gaithersburg, MD) webcast (10:23) available at http://www.nist.gov/oles/forensics/nist-forensics-2011-webcast.cfm

MC Kline and DL Duewer. “Metrology for Huntington’s Disease PCR Assays: Repeat Regions to Avoid,” Poster #G19 at Association for Molecular Pathology Annual Meeting on Genomic Medicine, (Long Beach, CA), October 25-27, 2012 [Click Here for .pdf]

RJ Haynes, MJ Holden, MC Kline, and JM Butler. “Model for Production of Nucleic Acid-Based NIST Standard Reference Materials for Clinical Diagnostics,” Poster #ID62 at Association for Molecular Pathology Annual Meeting on Genomic Medicine, (Long Beach, CA), October 25-27, 2012 [Click Here for .pdf]

RJ Haynes. “Principles of Digital PCR and Measurement Issues: The certification of Cytomegalovirus Standard Reference Material (SRM 2366) as a model for future SRMs” Digital PCR Applications and Advances (San Diego, CA), October 15-16, 2012 [Click Here for .pdf]

RJ Haynes, MJ Holden, MC Kline,and JM Butler. Current and Future Viral DNA Standard Reference Materials: Cytomegalovirus as a Model. Poster at 28th Clinical Virology Symposium, (Daytona Beach, FL), April 22-25, 2012 [Click Here for .pdf]

 

2011 Publications

MJ Holden, et. al. "Molecular diagnostics: harmonization through reference materials, documentary standards and proficiency testing." Expert Rev. Mol. Diagn. 11(7), 741–755 (2011) [Click Here for .pdf]


2010 Publications

RM Madej, J Davis, MJ Holden, S Kwang,
E Labourier, and GJ Schneider. "International Standards and Reference Materials for Quantitative Molecular Infectious Disease Testing." J Mol Diagn. 2010 Mar;12(2):133-43. [Click Here for .pdf]

 

2010 Presentations

JM Butler. "Dialogue with NIST: Standard Reference Materials for Molecular Diagnostics," Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

MC Kline and JM Butler. "Cautionary Considerations When Exploring Cell Lines as Potential Reference Materials," Poster #OTH05 at Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

MC Kline, DL Duewer, and JM Butler. "Characterizing the electrophoretic mobility of Huntington Disease Alleles 13-77: Are the deviations from the theoretical values intrinsic or internal sizing artifacts?" Poster #G09 at Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

RJ Haynes, MJ Holden, MC Kline, and JM Butler. "NIST Candidate Standard Reference Material (SRM): Cytomegalovirus DNA," Poster #ID19 at Association for Molecular Pathology Annual Meeting, (San Jose, CA), November 18-20, 2010 [Click Here for .pdf]

 

2009 Presentations

RJ Haynes, MJ Holden, MC Kline, and JM Butler. "Cytomegalovirus Standard Reference Material® Development for Nucleic Acid Amplification Technologies," Poster at Next Generation Dx Summit (Washington D.C.) August 10-12, 2009 [Click Here for pdf]

RJ Haynes, MJ Holden, MC Kline, and JM Butler. "Cytomegalovirus Standard Reference Material Development for Nucleic Acid Amplification Technologies" Poster #ID20 at Association for Molecular Pathology Annual Meeting, (Kissimmee, FL), November 19-22, 2009 [Click Here for pdf]

MC Kline, MJ Holden, RJ Haynes, AE Decker*, CR Hill,JW Redman, PM Vallone,and JM Butler. "Emerging Efforts in Clinical Genetics by the Applied Genetics Group at the National Institute of Standards and Technology," Poster #G47 at Association for Molecular Pathology Annual Meeting, (Kissimmee, FL), November 19-22, 2009 [Click Here for pdf]

MC Kline, AE Decker*, DL Duewer and JM Butler. "A NIST Standard Reference Material for Huntington Disease Alleles," Poster #G48 at Association for Molecular Pathology Annual Meeting, (Kissimmee, FL), November 19-22, 2009 [Click Here for pdf]



 

 

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Contact

Dr. Peter M. Vallone
peter.vallone@nist.gov
301-975-4872