Richard received his Ph.D. in Chemistry at Washington University in St. Louis in 2012. His thesis research was focused on developing and implementing mass spectrometry-based covalent labeling strategies (hydrogen/deuterium exchange or H/DX) to study protein-ligand interactions and structure. He is now working at NIST as a NRC postdoctoral fellow under Dr. Jeffrey Hudgens, where he continues to utilize H/DX-MS as a tool to study the effects of glycosylation on conformation and dynamics of glycoproteins. This study can help understand the importance of protein post-translational modification (PTMs) on multi-domain, multi-subunit proteins and improve future biological drug design.
Selected Publications
Huang, R.Y.C., Wen, J., Blankenship, R.E., and Gross, M.L. "Hydrogen-deuterium exchange mass spectrometry reveals the interaction of Fenna-Matthews-Olson protein and chlorosome CsmA protein", Biochemistry, 2012, 51, 187-193.
Huang, R.Y.C., Garai, K., Frieden, C., and Gross, M.L. "Hydrogen/Deuterium Exchange and Electron-Transfer Dissociation Mass Spectrometry Determine the Interface and Dynamics of Apolipoprotein E Oligomerization", Biochemistry, 2011, 50, 9273-9282.
Huang, R.Y.C., Rempel, D.L., and Gross, M.L. "HD Exchange and PLIMSTEX Determine the Affinities and Order of Binding of Ca2+ with Troponin C", Biochemistry, 2011, 50, 5426-5435.
Huang, R.Y.C., Laing, J.G., Kanter, E.M., Berthoud, V.M., Bao, M., Rohrs, H.W., Townsend, R.R., and Yamada, K.A. "Identification of CaMKII Phosphorylation Sites in Connexin43 by High-Resolution Mass Spectrometry", J. Proteome Res, 2011, 10, 1098-1109.
Liu, H., Huang, R.Y.C., Chen, J., Gross, M.L., and Pakrasi, H.B. "Psb27, a transiently associated protein, binds to the chlorophyll binding protein CP43 in photosystem II assembly intermediates", Proc. Natl. Acad. Sci. U.S.A., 2011, 108, 18536-18541.
Sperry, J.B., Huang, R.Y.C., Zhu, M.M., Rempel, D.L., and Gross, M.L. "Hydrophobic peptides affect binding of calmodulin and Ca2+ as explored by H/D amide exchange and mass spectrometry", Int. J. Mass Spectrom., 2011, 302, 85-92.
Zhang, H., Huang, R.Y.C., Jalili, P.R., Irungu, J.W., Nicol, G.R., Ray, K.B., Rohrs, H.W., and Gross, M.L. "Improved Mass Spectrometric Characterization of Protein Glycosylation Reveals Unusual Glycosylation of Maize-Derived Bovine Trypsin", Anal. Chem., 2010, 82, 10095-10101.
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Position:
Postdoctoral Fellow
Biomolecular Measurement Division
Bioprocess Measurement Group
Employment History:
National Institute of Standards and Technology (NIST), NRC Postdoctoral Research Associate (2012-Present)
Education:
Ph.D., Chemistry, 2012, Washington University in St. Louis
B.S., Chemistry, 2005, National Central University, Taiwan
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