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Dr. Jeffrey W. Hudgens

Research Interests

  • H/D exchange mass spectrometry (HDX-MS) for biopharmaceutical analysis
  • Dynamics of ligand-membrane protein and ligand-transmembrane protein interactions
  • Comparability of innovator and biosimilar drugs measured with HDX-MS
  • Improvement of measurement and quality assurance protocols for HDX-MS
  • Covalent and noncovalent bonding interactions of biological drugs (and other biological materials) with surfaces and nanoparticles
  • High-performance mass spectrometry

NOTE: Dr. Hudgens has an NRC Postdoctoral Opportunity in Research involving Hydrogen/deuterium (HDX) exchange mass spectrometry (Qualified candidates should contact Dr. Hudgens for more information). The next deadlines for submission of applications are 1 AUG 2016 and 1 FEB 2017.


Dr. Jeffrey W. Hudgens' research investigates the precision of hydrogen-deuterium exchange mass spectrometry (HDX-MS) and its application to the measurement of protein-ligand and glycoprotein-ligand interactions, the dynamical structures of mAbs, lipid-protein complexes, and transmembrane protein interactions. Dr. Hudgens received his B.S. in Chemistry from Miami University (Ohio) and his Ph.D. in Physical Chemistry from the University of Illinois at Urbana. He was awarded a National Research Council Postdoctoral Associateship at the Naval Research Laboratory. He remained at NRL for another seven years as a staff scientist, where he pioneered research concerning resonance enhanced multiphoton ionization (REMPI) spectroscopy of free radicals, the fundamentals of infrared multiphoton dissociation (IRMPD) and inverse radiationless processes. In 1984 he moved to the National Bureau of Standards (now NIST). At NIST Dr. Hudgens inaugurated early research that employed cavity ringdown spectroscopy as a tool for the study of chemical kinetics. He invented evanescent-wave cavity ringdown spectroscopy of liquid films. With mass spectrometry he has conducted numerous studies, which determined the structure of a metal-sugar complex and deduced reaction networks that form nearly monodisperse gold:phosphine nanoclusters. Dr. Hudgens' 130+ scientific publications and two patents span many disciplines including chemical dynamics, chemical kinetics, chemical thermodynamics, inorganic biochemistry, laser chemistry, optical spectroscopy, mass spectrometry, and nanochemistry. Dr. Hudgens is a Fellow of the American Physical Society.

Selected Honors and Awards

  • Chemical Science and Technology Laboratory (CSTL) Technical Achievement Award for the Invention of Evanescent Wave Cavity Ringdown Spectroscopy, 1998
  • Fellow of the American Physical Society, 1995
  • National Bureau of Standards Bronze Medal for Research, 1987
  • Naval Research Laboratory Invention Award, 1983 (for a method in mass spectrometry)

Conferences and Workshops Organized

  • Organizer and Co-chairman of the Third, Fourth, and  Fifth International Conference on Chemical Kinetics that convened in July of 1997, 2001, 2005 at NIST in Gaithersburg, MD.
  • Organizing committee member of the "Workshop on Combustion Simulation Databases for Real Transportation Fuels, 2003". Subsequently, Dr. Hudgens acted as the editor and as a co-author of the 2003 NIST workshop report.
  • Organizer of workshops in 2002 and 2003 that brought together US and Hungarian scientists from leading scientific institutions enabling establishment of a Web-accessible database of critically evaluated thermodynamic data for free radicals. These workshops were sponsored by the US-Hungarian Research Council and the International Union of Pure and Applied Chemistry (IUPAC).

Selected Publications  

(A complete list of publications is available Here.)

  • "Cholesterol Hydroxylase CYP46A1: Mapping of the Allosteric Site for Efavirenz, a Drug that Stimulates Enzyme Activity" K. W. Anderson, N. Mast, J. W. Hudgens, J. B. Lin, I. V. Turko, and I. A. Pikuleva, Journal of Biological Chemistry, 291, 11876-11886 (2016). DOI: 10.1074/jbc.M116.723577
  • "Mapping Protein-Ligand Interactions with Proteolytic-Fragmentation, Hydrogen/Deuterium Exchange-Mass Spectrometry" E. S.Gallagher and J. W. Hudgens, Methods in Enzymology, 566, 357-404. (2016). DOI: 10.1016/bs.mie.2015.08.010
  • Book Chapter:"Method Validation and Standards in Hydrogen Exchange Mass Spectrometry" J. W. Hudgens, R. Y.-C. Huang and E. D'Ambro in Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Techniques and Applications; David Weis, ed. (Wiley, 2016).
  • Book Chapter: "Emerging Technologies to Assess the Higher-Order Structure of Monoclonal Antibodies" J.P. Marino, R.G. Brinson, J.W. Hudgens, J.E. Ladner, D.T. Gallagher, E. S. Gallagher, L.W. Arbogast, and R. Y.-C. Huang In State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization Volume 3. Defining the Next Generation of Analytical and Biophysical Techniques, Schiel, J.; Borisov, O.; Davis, D., eds. American Chemical Society: 2015; Vol. 1202, pp 17-43. . DOI: 10.1021/bk-2015-1202.ch002
  • "Characterization of AlgMsp, an Alginate Lyase from Microbulbifer sp. 6532A" S. M. Swift, J. W. Hudgens, R. D. Heselpoth, P. M. Bales, and D. C. Nelson, PLoS ONE 9(11), e112939 (2014). DOI: 10.1371/journal.pone.0112939
  • "A Novel Mechanism for Regulating the Activity of Proliferating Cell Nuclear Antigen by a Small Protein" Z. Li, R. Y.-C. Huang, D. C. Yopp, T. H. Hileman, T. J. Santangelo, J. Hurwitz, J. W. Hudgens, and Z. Kelman, Nucleic Acids Research, 42, 5776-5789 (2014). DOI: 10.1093/nar/gku239
  • "Effects of Desialylation on Human α1-Acid Glycoprotein-Ligand Interactions" R. Y-C Huang and J. W. Hudgens, Biochemistry, 52, 7127–7136 (2013). DOI: 10.1021/bi4011094
  • "Identification of Active Sites of Biomolecules II: Saccharide and Transition Metal Ion in Aqueous Solution" O. Coskuner, D. E. Bergeron, L. Rincon, J. W. Hudgens, and C. A. Gonzalez, J. Phys. Chem. A 113, 2491–2499 (2009). DOI:10.1021/jp805747f

Nanocluster Publications

  • Surface Mediated Assembly of Small, Metastable Gold Nanoclusters" J. M. Pettibone, W. A. Osborn, K. Rykaczewski, A. A. Talin, J. E. Bonevich, and J. W. Hudgens and M. D. Allendorf, Nanoscale, 5, 6558-6566 (2013).  DOI: 10.1039/c3nr01708g
  • "Reaction Network Governing Diphosphine-Protected Gold Nanocluster Formation from Nascent Cationic Platforms" J. M. Pettibone and J. W. Hudgens, Phys. Chem. Chem. Phys. 14, 4142-4154 (2012). DOI:10.1039/C2CP22865C
  • "Predictive Gold Nanocluster Formation Controlled by Metal-ligand Complexes" J. M. Pettibone and J. W. Hudgens, Small 8, 715-725 (2012). DOI: 10.1002/smll.201101777
  • "Reaction Mechanism Governing the Formation of 1,3-bis(diphenylphosphino)propane-protected Gold Nanoclusters" J. W. Hudgens, J. M. Pettibone, T. P. Senftle, and R. N. Bratton, Inorg. Chem. 50, 10178–10189 (2011). DOI: 10.1021/ic2018506
  • "Gold Cluster Formation with Phosphine Ligands: Etching as a Size-Selective Synthetic Pathway for Small Clusters?" J. M. Pettibone and J. W. Hudgens, ACS Nano 5, 2989–3002 (2011). DOI: 10.1021/nn200053b.
Image of Dr. Jeffrey W. Hudgens


Research Chemist
Biomolecular Measurement Division
Bioprocess Measurements Group at the Institute for Bioscience & Biotechnology Research

Employment History:

OCT 2012 - present
Biomolecular Measurement Division
Rockville, MD
OCT 2010 - SEP 2012
Biochemical Science Division
NIST, Gaithersburg, MD
OCT 2009 - SEP 2010
Chemical and Biochemical Reference Data Division
NIST, Gaithersburg, MD
JUL 2003 - SEP 2009
Physical and Chemical Properties Division
NIST, Gaithersburg, MD  
MAR 2001 - JUN 2003
Advanced Technology Program (Detail) 
NIST, Gaithersburg, MD 
AUG 1997 - FEB 2001
Physical and Chemical Properties Division
NIST, Gaithersburg, MD 
MAR 1997 - JUL 1997
Advanced Technology Program (Detail)
NIST, Gaithersburg, MD 
OCT 1990 - FEB 1997
Chemical Kinetics and Thermodynamics Division
NIST, Gaithersburg, MD 
MAR 1984 - SEP 1990
Chemical Kinetics Division
National Bureau of Standards
Gaithersburg, MD 
OCT 1981- FEB 1984
Chemical Kinetics Section
Naval Research Laboratory
Washington, DC
MAR 1977 - SEP 1981
Mass Spectrometry Section
Naval Research Laboratory
Washington, DC 
NOV 1975 - FEB 1977
National Research Council Postdoctoral Fellow (NRC/NRL)
Chemical Kinetics Section
Naval Research Laboratory
Washington, DC

Society Memberships

  • American Chemical Society
  • American Physical Society (Fellow)
  • American Society for Mass Spectrometry
  • CASSS - An International Separation Science Society


Miami University, Ohio
B.S., Chemistry  
University of Illinois at Urbana, IL
Ph.D., Physical Chemistry

Phone: 240 314-6485
Email: jeffrey.hudgens@nist.gov
Fax: 240 314-6255