Meiyao Wang

My research focuses on quantification and characterization of membrane-associated proteins (MAPs) in complex biological samples using liquid chromatography (LC) coupled tandem mass spectrometry (MS) with isotope labeled internal standards. By using our optimized sample preparation protocol combined with nanoLC - multiple reaction monitoring (MRM) /MS, we successfully performed quantitative analysis for multiple target MAPs, including the Alzheimer's disease risk factor apolipoprotein E4 (apoE4) isoform from human frontal cortex, the clinical cell surface marker cluster of differentiation 4 (CD4) from human T lymphocytes and cholesterol-metabolizing cytochrome P450s with their redox partners from human temporal lobe and retina. Our study addressed the following issues: 1) development of various types of isotope-labeled internal standards for quantification of the target MAPs; 2) quantitative differentiation of specific protein isoforms; and 3) quantitative analysis of protein cleavage and fragment accumulation associated with human disease. We demonstrate that our quantitative workflow based on LC-MRM/MS can be used for absolute quantification of MAPs as well as for the exploration of basic mechanisms of human diseases. We believe this method can be widely applied for other MAPs and MAP-related protein analyses.

Recent publications

"Quantifying the Cluster of Differentiation 4 Receptor Density on Human T Lymphocytes Using Multiple Reaction Monitoring Mass Spectrometry" M. Wang*, H. He, I. V. Turko, K.W. Phinney and L. Wang, Analytical Chemistry, 2013, 85 (13), pp 1773-1777.

"¹⁵N-Labeled Full-Length Apolipoprotein E4 as Internal Standard for Mass Spectrometry Quantification of Apolipoprotein E Isoforms" M. Wang, J. Chen and I. V. Turko, Analytical Chemistry, 2012, 84 (19), pp 8340–8344.

"Sample Pre-Fractionation for Mass Spectrometry Quantification of Low-Abundance Membrane Proteins", M. Wang, G. Heo, S. Omarova, I. Pikuleva, and I. V. Turko, Analytical Chemistry, 2012, 84, 5186-5191.  

"Quantification of Amyloid Precursor Protein Isoforms Using QconCAT Internal Standard", J Chen, M. Wang and I.V. Turko, Analytical Chemistry, 2013, 85 (1), pp 303–307.

"Mass Spectrometry Quantification of Clusterin in Brain Tissues", J. Chen, M. Wang, and I. V. Turko, Molecular Neurodegeneration, 2012, 7:41.

"Mass Spectrometry Quantification Revealed Accumulation of C-terminal Fragment of Apolipoprotein E in the Alzheimer's Frontal Cortex" M. Wang and I.V. Turko, PLoS One, Jan, 2013, in press

Awards

Young Investigator Award, the 4^th Mass Spectrometry Application to the Clinical LAB 2012 conference
Young Investigator Award, the 5^th Mass Spectrometry Application to the Clinical LAB 2013 conference