Dr. Antony K. Chen

INTRODUCTION

Projects

1) Investigating the role of mitochondrial bioenergetics and distribution in living cells crawling on ECM of different stiffness.

2) Developing novel molecular imaging probes to sensitively monitor the trafficking, distribution and the level of specific biomolecules in living cells.

PUBLICATIONS  

Chen, A.K. and Tsourkas A. Developing Molecular-Beacon Constructs for Improved Live-Cell Analysis and Imaging of RNA (Review). Am. Biotechnol Lab. Accepted.

Chen, A.K., Davydenko, O., Behlke, M., and Tsourkas A. Ratiometric Bi-Molecular Beacons: A New Probe for Imaging RNA in Single Living Cells. Submitted to Nucleic Acids Res.

Crayton, S., Chen, A.K., Cheng, Z., and Tsourkas, A. "Molecular Imaging". In Comprehensive Biomaterials, edited by L. Hutchins. Elsevier Science/Academic Press. Submitted.

Chen, A.K., Rhee, W.J., Bao G. and Tsourkas A. Delivery of Molecular Beacons for Live-cell Imaging and Analysis of RNA. Methods Mol Biol (Review). In Press.

Cheng, Z., Chen, A.K., Lee H-Y., and Tsourkas, A. Examination of Folate-Targeted Liposomes with Encapsulated Poly(2-Propylacrylic acid) as a pH-responsive Nanoplatform for Cytosolic Drug Delivery. Small. Accepted.

Chen, A.K., Behlke, M., and Tsourkas, A. Subcellular Trafficking and Functionality of 2'-O-methyl and 2'-O-methy-phosphorothioate Molecular Beacons. Nucleic Acids Res. 2009; 37 (22): e149.

Chen, A.K. and Tsourkas, A. Imaging RNA in Living Cells with Molecular Beacons: Current Perspectives and Challenges. J. Innov. Opt. Health Sc (Review). 2009; 2 (4): 315-324.

Chen, A.K., Cheng, Z., Behlke, M., and Tsourkas, A. "Assessing the Sensitivity of Commercially Available Fluorophores to the Intracellular Environment". Anal Chem. 2008; 80 (19): 7437-44.

Chen, A.K., Behlke, M., and Tsourkas, A. "Efficient Cytosolic Delivery of Molecular Beacon Conjugates and Flow Cytometric Analysis of Target RNA". Nucleic Acids Res. 2008; 36 (12): e69.

Chen, A.K., Behlke, M., and Tsourkas, A. "Avoiding False-Positive Signals with Nuclease-Vulnerable Molecular Beacons in Single Living Cells". Nucleic Acids Res. 2007; 35 (16): e105.

Elias, D.R., Thorek, D.L., Chen, A.K., Czupryna, J., and Tsourkas, A. "In vivo Imaging of Cancer Biomarkers Using Activatable Molecular Probes". Cancer Biomarkers (Review). 2008; 4 (6): 287-305.

Chen, A.K., Latz, M.I., Sobolewski, P., and Frangos, J.A. "Evidence for the Role of G-Proteins in Flow Stimulation of Dinoflagellate Bioluminescence." Am J Physiol Regul Integr Comp Physiol. 2007; 292(5): R2020-7.

Thorek, D.L.J., Chen, A.K., Czupryna, J., and Tsourkas, A. "Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging." Ann Biomed Eng. 2006; Jan;34(1):23-38 (Review).

Thorek, D.L.J., Czupryna, J., Chen, A.K., Tsourkas, A. "Molecular Imaging of Cancer with Superparamagnetic Iron Oxide Nanoparticles." In Cancer Imaging, edited by M.A. Hayat. Elsevier Science/Academic Press.

Chen, A.K., Latz, M.I., and Frangos, J.A. "The Use of Dinoflagellate Bioluminescence to Characterize Cell Stimulation in Bioreactors." Biotechnol Bioeng. 2003; 83(1): 93-103.