Dr. Hua-Jun He

My research at NIST focuses on the development of high throughput based methods for protein and DNA biomarkers, i.e. a fluorescence resonance energy transfer (FRET) based method for the detection of DNA binding activities of nuclear factor κB and an antibody array for sensing the insulin signaling pathway. Recently, I am working on the standardization of cardiac Troponin I measurement project and glycoprotein metrology for efficient biomanufacturing of safe and effective therapeutic proteins.

Memberships and Committees:

The Endocrine Society
America Diabetes Association (ADA)

Selected Publications:

1. Sige Zou, Hua-Jun He, Yaping Zong, Leming Shi, Lili Wang. DNA-Microarrays: Applications, Future Trends, and Need for Standardization, In "Springer Series on Fluorescence, Volume 6. Standardization and QualityAssurance in Fluorescence Measurements II", Ute Resch-Genger (ed), Springer-Verlag Berlin Heidelberg, 2008, pp215-237.

2. Hua-Jun He, Richard Pires, Tie-Nian Zhu, Anhong Zhou, Adolfas K. Gaigalas, Sige Zou, Lili Wang. Fluorescence resonance energy transfer based method for detection of DNA binding activities of nuclear factor κB. BioTechniques, 2007, 43(1):93-98.

3. Wang L, Cole KD, Peterson A, Hua-Jun He, Gaigalas AK, Zong Y. Monoclonal antibody selection for interleukin-4 quantification using suspension arrays and forward-phase protein microarrays. Journal of Proteome Research, 2007, 6(12):4720-4727.

4. Tie-Nian Zhu, Hua-Jun He, Sutapa Kole, Theresa D’Souza, Rachana Agarwal, Patrice J. Morin, Michel Bernier. Filamin A-mediated downregulation of the exchange factor Ras-GRF1 correlates with decreased matrix metalloproteinase-9 expression in human melanoma cells. The Journal of Biological Chemistry, 2007, 282(20):14816-26.

5. L. Wang, K. D. Cole, Hua-Jun He, D. K. Hancock, A. K. Gaigalas, Y. Zong. Comparison of Ovalbumin Quantification Using Forward-Phase Protein Microarrays and Suspension Arrays. Journal of Proteome Research, 2006, 5 (7):1770 -1775.

6. Hua-Jun He, Tie-Nian Zhu, Yi Xie, Jinshui Fan, Sutapa Kole, Satya Saxena, Michel Bernier. Pyrrolidine dithiocarbamate inhibits interleukin-6 signaling through impaired STAT3 activation and association with transcriptional coactivators in hepatocytes. The Journal of Biological Chemistry, 2006, 281(42):31369-79.

7. Michel Bernier, Hua-Jun He, Yong-Kook Kwon, Hyeung-Jin Jang. The roles of phospholipase C-gamma1 and actin-binding protein filamin A in signal transduction of the insulin receptor, In "Vitamins and Hormones", Gerald Litwack(ed), Academic Press, New York, 2004, Vol 69, pp221-247.