Dr. Gregory A. Cooksey

My interests lie in developing microfluidics systems that enable real-time imaging and control of the microenvironments of single cells and groups of cells in high-throughput experiments. Important aspects of this work involve creating macro-to-micro interfaces, designing schemes to deliver and manipulate multiple fluid flows inside microchambers, and developing image analysis tools for real-time system feedback and quantitative measurements within regions of interest. Currently, we are conducting experiments in a high-throughput microfluidic toxicity platform for timelapse observation of 128-culture chambers (see photo): multiple chemical (toxin) gradients and different biomarkers are multiplexed for a total of 128 unique exposure conditions.

128-chamber design

Awards:
National Research Council Postdoctoral Fellowship, NIST, 2007
NSF IGERT Graduate Fellowship in Nanotechnology, UW, 2004
Whitaker Foundation Fellowship in Biomedical Engineering, 1999
Barry M. Goldwater Scholar, 1997
Nikon Small World 2008, Juried, Image of Distinction, 2008
“Images from Science 2008,” 2 photos, Juried, 2008
Featured Photos, Science Illustrated (2009) and Discover Magazine (2008)

Selected Publications:
Cooksey GA, Plant AL, Atencia J. (2009) “A vacuum manifold for rapid world-to-chip connectivity of complex PDMS microdevices.” Lab on a Chip, DOI:10.1039/B820683J

Cooksey GA, Sip CG, Albert Folch A. (2009). “A multi-purpose microfluidic perfusion system with combinatorial choice of inputs, mixtures, gradient patterns, and flow rates.” Lab on a Chip, DOI: 10.1039/B806803H.

Lam EW, Cooksey GA, Finlayson BA, Folch A. (2006). “Microfluidic circuits with tunable flow resistances.” Applied Physics Letters 89, 164105.

Talley CE, Cooksey GA, Dunn RC. (1996) “High resolution fluorescence imaging with cantilevered near-field fiber optic probes” Applied Physics Letters, 69, 3809-3811.