Fatima Sequeira has been implementing a cytotoxicity assay for better understanding of how known chemical toxins influence protein expression. Through the use of automated microscopy and a monkey kidney cell line which expresses destabilized green florescent protein, she has been able to monitor the effect these reference toxins have on the system. Further interest in performing a cross-species comparison of these toxins in yeast cells will provide insight if these toxins work through similar pathways in both organisms.
In the next year she plans to start work on the development of a quantitative metric for a dual component glycosylation probe. The first goals of this project will be establish, evaluate, and optimize the performance of dual components of the system. Once this is done she envisions that this system could be used as a biomarker for cancer cells undergoing hypoxia.
Fatima obtained her doctoral degree from the State University of New York at Buffalo in synthetic organic chemistry. During her time in Dr. Chemler's group she developed new chemical methods to provide efficient and concise synthesis of nitrogen heterocycles. The research strived to expand and demonstrate the utility of copper(II)-difunctionalization reactions of alkenes for the formation of nitrogen heterocycles. These methods provide a viable synthetic route to chiral indolines, which are prevalent in medicinal chemistry research.
F. C. Sequiera, S. R. Chemler (2012) "Stereoselective Synthesis of Morpholines Via Copper(II)-Promoted Alkene Aminooxygenation," Org. Lett. 14, 4482-5
F. C. Sequeira, M. T. Bovino, A. J. Chipre, S. R. Chemler (2012) "Multigram Synthesis of a Chiral Substituted Indoline Via Copper-Catalyzed Alkene Aminooxygenation," Synthesis, 44, 1481-1484.
F. C. Sequeira, B. W. Turnpenny, S. R. Chemler (2010), "Copper-Promoted and Copper-Catalyzed Intermolecular Alkene Diamination," Angew. Chem. Int. Ed. 49, 6365-6368.
Biosystems and Biomaterials Division
Cell Systems Science Group
(2012-present) NRC Postdoc, National Institute of Standards and Technology, Gaithersburg, MD
Ph.D. Organic Chemistry, State University of New York at Buffalo, 2012.