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Sandra M. Da Silva

My current research is focused on understanding the fundamental parameters affecting biological sampling to: (1) develop new measurement capabilities for assessing the extent of contamination and (2) evaluate the effectiveness of decontamination procedures. The study is approached thru fundamental parameters such as adhesion forces, essential to understanding the nature of variability present in surface sampling methodologies. Parameters impacting adhesion include surface properties, environmental conditions and sampling materials. This work contributes to the development of surface sampling standards fundamental to assessing threats to human health as well as for microbial community control technologies.

Previously, my research focused on (1) on the mechanistic study of the peroxyoxalate reaction, by proposing 1,2-dioxetanodione as a key high-energy intermediate. An understanding of the peroxyoxalate mechanism has implications on the development of new analytical e bioanalytical tools involving chemiluminescence. (2) Photodynamic Therapy (PDT) working on synthesis and characterization of mitochondrial fluorescent dyes based on rhodamine derivatives for application in tumor cells and (3) on the development of a luminescent CdSe/ZnS quantum dot based FRET sensors for cellular proteolytic activity of adenocarcinoma pancreatic cells (CAPAN-2) in microfluidic device. The FRET pair was composed by CdSe/ZnS quantum dots (QD) and rhodamine-peptide-PAH poly(allylamine hydrochloride) as donor and acceptor respectively embedded into a PEM (polyelectrolyte multilayer) film.


Fellowships:

1999-2000: Fapesp (Fundação de Amparo à Pesquisa do Estado de São Paulo) - Brazil

2001-2004: CNPq (Conselho Nacional de Pesquisa) - Brazil

05/2003-08/2003: DAAD (Deutcher Akademischer Austauschdienst in Organic Chemistry - Institute - Friedrich-Schiller University, Jena, Germany.


Publications:

Stevani, C.V., Silva, S.M. & Baader, W.J. "Studies on the Involvement of the CIEEL Sequence in the Peroxyoxalate Reaction"; em: Bioluminescence and Chemiluminescence, Perspectives for the 21st Century, eds: Roda, A., Pazzagli, M., Kricka, L.J. & Stanley, P.E., John Wiley & Sons, Bologna, Italy, 33-38, 1999.

Stevani. C.V, Silva, S.M. & Baader, W.J "Studies on the Mechanism of the Excitation Step in Peroxyoxalate Chemiluminescence" Eur. J. Org. Chem., 2000, 4037-4046.

Silva, S.M., Wagner, K., Weiss, D., Beckert, R. & Baader, W.J. " Studies on the Chemiexcitation Step in Peroxyoxalate Chemiluminescence Using Steroid-Substituted Activators" Luminescence, 2002, 17, 362-369.

Silva, S.M., Jr., F.C., Oyamaguchi, K.H., Ciscato, L.F.L.M., Stevani, C.V. & Baader, W.J. "Kinetic Studies on the Peroxyoxalate Chemiluminescence Reaction: Determination Of the Cyclization Rate Constant" Luminescence, 2002, 17, 313-320.

Martins TS, Araújo AAS, Silva SM, Matos JR, Isolani PC & Vicentini G, "Syntesis, Characterization, Spectroscopy Study and Thermal Analysis of Rare Earth Picrate Complexes with L-Arginine" J. Sol. State. Chem., 2003, 171, 212-216.

Crivat, G., Da Silva, SM.,Reyes, DR., Locascio, L., Gaitan, M., Rosenweig N & Rosenzweig Z., "Quantum Dot FRET-Based Probes in Thin Films Grown in Microfluidic Channels" J. Am. Chem. Soc, 2010, 132, 1460-1461.

Belostotsky, I., Da Silva, SM., Paez, MG and Indig, GI. "Mitochondrial Targeting for Photochemotherapy. Can Selective Tumor Cell Killing be predicted Based on n-octanol/water Distribution Coefficients?" Biotech & Histochem, 2010, 1-13.

Da Silva, SM., Filliben, JJ and Morrow, JB., "Role of Surface Chemistry in the Extraction of Bacillus anthracis Sterne from Wipe Materials". (Submitted to Applied Environmental Microbiology).

sandra_dasilva

Position:

Guest Researcher
Biochemical Science Division
Bioassay Methods Group

Employment History:

2008-present: PostDoctoral Researcher/Guest Researcher, Biochemical Science Division, NIST, Maryland.
2006-2008: PostDoctoral Researcher, Chemistry department - University of New Orleans - New Orleans.
2004-2006: PostDoctoral Reseracher, Biochemistry Department - University of Wisconsin – Milwaukee.

Education:

PhD in Organic Chemistry, University of São Paulo, Brazil, 2004.

Contact

Phone: 301-975-4665
Email: sandra.dasilva@nist.gov