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Young Jong Lee, Ph.D.

Research Interests

  • Label-Free Chemical Imaging of Cells and Tissues Using Broadband CARS Microscopy
  • Coherent Raman Spectroscopy to Rapidly Characterize the Higher Order Structure of Proteins
  • Statistical/Quantitative Image Analysis of Hyperspectral Raman Image Data
  • Molecular Structure of Complex Materials by in-situ Imaging Chemical Composition and Orientation

We use CARS microscopy for label-free chemical imaging of biological and complex materials systems. We have improved the sensitivity of broadband CARS to obtain Raman images of cells and tissues much faster than conventional Raman microscopy. We have demonstrated that CARS imaging can measure the degree of differentiation of stem cells (Lee et al. 2014) and track chemical signaling pathways in white blood cells (Afonso et al. 2012). This highly sensitive chemical imaging method is also being used to provide 3-D chemical and orientational morphology for other complex materials systems, including polymer blends (Lee et al. 2012, 2011), biofilms, and hydrogels.

We are developing a new coherent Raman spectroscopic method to characterize the higher order structure of complex biomolecules, such as therapeutic proteins and their aggregates.


Postdoctoral Opportunities

NRC Postdoctoral Fellowship: 2-year fellowship, US citizens only. Please visit two following opportunities and contact me if interested.

 

Publications at NIST

  • N. V. Gohad, N. Aldred, C. M. Hartshorn, Y. J. Lee, M. T. Cicerone, B. Orihuela, A. S. Clare, D. Rittschof, A. S. Mount, Nat. Commun. published online (2014). Synergistic Roles for Lipids and Proteins in the Permanent Adhesive of Barnacle Larvae
  • C. H. Camp Jr., Y. J. Lee, J. M. Meddleston, C. M. Hartshorn, A. R. Hight Walker, J. Rich, J. Lathia, M. T. Cicerone, Nat. Photonics published online (2014). High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
  • Y. J. Lee, S. L. Vega, P. J. Patel, K. A. Aamer, P. V. Moghe, M. T. Cicerone, Tissue Eng. Part C, 20, 562 (2014). Quantitative, Label-Free Characterization of Stem Cell Differentiation at the Single-Cell Level by Broadband Coherent Anti-Stokes Raman Scattering Microscopy
  • M. Hartshorn, Y. J. Lee, C. H. Camp, Z. Liu, J. M. Heddleston, N. Canfield, T. A. Rhodes, A. R. Hight Walker, P. Marsac, M. T. Cicerone, Anal. Chem. 85, 8102 (2013). Multicomponent Chemical Imaging of Pharmaceutical Solid Dosage Forms with Broadband CARS Microscopy
  • Y. J. Lee, C. R. Snyder, A. M. Forster, M. T. Cicerone, W. Wu, ACS Macro Lett. 1, 1347 (2012). Imaging the Molecular Structure of Polyethylene Blends with Broadband Coherent Raman Microscopy
  • J. P. Dunkers, Y. J. Lee, K. Chatterjee, Biomaterials 33, 2119 (2012) Single Cell Viability Measurements in 3D Scaffolds Using in situ Label Free Imaging by Optical Coherence Microscopy
  • P. V. Afonso, M. Janka-Junttila, Y. J. Lee, C. P. McCann, C. M. Oliver, K. A. Aamer, W. Losert, M. T. Cicerone, C. A. Parent, Developmental Cell 22, 1079 (2012). LTB4 Is a Signal Relay Molecule for Neutrophil Chemotaxis
  • M. T. Cicerone, K. A. Aamer, Y. J. Lee, E. Vartianen, J. Raman Spectrosc. 43, 637 (2012) Maximum Entropy and Time-Domain Kramers-Kronig Phase Retrieval Approaches are Functionally Equivalent for CARS Micro-Spectroscopy
  • Y. J. Lee, D. Moon, K. B. Migler, M. T. Cicerone, Anal. Chem. 83, 2733 (2011). Quantitative Image Analysis of Broadband CARS Hyperspectral Images of Polymer Blends
  • Y. J. Lee, S. H. Parekh, F. A. Fagan, M. T. Cicerone, Phys. Rev. B 82, 165432 (2010). Phonon dephasing and population decay dynamics of the G-band of semiconducting single-wall carbon nanotubes
  • S. H. Parekh, Y. J. Lee, K. A. Aamer, M. T. Cicerone, Biophys. J. 99, 2695 (2010). Label-free cellular imaging by broadband coherent anti-Stokes Raman scattering microscopy
  • K. Chatterjee, S. Lin-Gibson, W. E. Wallace, S. H. Parekh, Y. J. Lee, M. T. Cicerone, M. F. Young, C. G. Simon, Jr., Biomaterials31, 5051 (2010). The Effect of 3D Hydrogel Scaffold Modulus on Osteoblast Differentiation and Mineralization Revealed by Combinatorial Screening Biomaterials
  • Y. J. Lee, S. H. Parekh, Y. H. Kim, M. T. Cicerone, Opt. Express 18, 4371 (2010). Optimized continuum from a photonic crystal fiber for broadband time-resolved coherent anti-Stokes Raman scattering
  • Y. Liu, Y. J. Lee, M. T. Cicerone, Opt. Lett. 34, 1363 (2009). Broadband CARS Spectral Phase Retrieval Using a Time-Domain Kramers-Kronig Transform
  • Y. J. Lee, M. T. Cicerone, Opt. Express 17, 123 (2009). Single-shot interferometric approach to background free broadband coherent anti-Stokes Raman scattering spectroscopy
  • Y. Liu, Y. J. Lee, M. T. Cicerone, J. Raman Spectrosc. 40, 726 (2009). Fast Extraction of Resonant Vibrational Response from CARS Spectra with Arbitrary Nonresonant Background
  • Y. J. Lee, M. T. Cicerone, Appl. Phys. Lett. 92, 041108 (2008). Vibrational Dephasing Time Imaging by Time-Resolved Broadband Coherent Anti-Stokes Raman Scattering Microscopy
  • Y. J. Lee, Y. Liu, M. T. Cicerone, Opt. Lett. 32, 3370 (2007). Characterization of 3-color CARS in a 2-pulse broadband CARS Spectrum 

Selected Publications before NIST: 

  • Y. J. Lee, D. Y. Kim, P. F. Barbara, J. Phys. Chem. B110, 9739 (2006). Effects of Sample Preparation and Excitation Conditions on the Single Molecule Spectroscopy of Conjugated Polymers
  • Y. J. Lee, S.-J. Park, A. J. Gesquiere, P. F. Barbara, Appl. Phys. Lett. 87, 051906 (2005). Probing a Molecular Interface in a Functioning Organic Diode
  • Y. J. Lee, D. Y. Kim, J. K. Grey, P. F. Barbara, ChemPhysChem 6, 2404 (2005). Variable Temperature Single-Molecule Dynamics of MEH–PPV
  • J. K. Grey, D. Y. Kim, Y. J. Lee, J. J. Gutierrez, N. Luong, J. P. Ferraris, P. F. Barbara, Angew. Chem. Int. Ed. 44, 6207 (2005). Single Molecule Studies of Electronic Energy Transfer in a Diblock Conjugated Polymer
  • P. F. Barbara, A. J. Gesquiere, S.-J. Park, Y. J. Lee, Acc. Chem. Res. 38, 602 (2005). Single Molecule Spectroscopy of Conjugated Polymers
  • Y. J. Lee, T. Zhang, P. F. Barbara, J. Phys. Chem. B 108, 5175 (2004). Kinetics of Electron Attachment to Reverse Micelles
  • Y. J. Lee, T. W. Kee, T. Zhang, P. F. Barbara, J. Phys. Chem. B 108, 3474 (2004). Pump–Probe Spectroscopy of the Hydrated Electron in Reverse Micelles
  • Y. J. Lee, S. H. Cho, N. J. Kim, S. K. Kim, Mol. Phys. 110, 1781 (2012). Experimental Determination of the Ratio of Partial Photoionization Cross Sections from Na 3p 2P3/2 by Polarization Anisotropy Quantum Beats
  • S. M. Jin, Y. J. Lee, J. Yu, S. K. Kim, Bull. Korean Chem. Soc. 25, 1829 (2004). Development of Femtosecond Stimulated Raman Spectroscopy: Stimulated Raman Gain via Elimination of Cross Phase Modulation
  • Y. J. Lee, N. W. Song, S. K. Kim, J. Phys.Chem. A 106, 5582 (2002). Photoluminescence of C60 and Its Photofragments in the Gas Phase
  • Y. J. Lee, S. H. Cho, N. J. Kim, S. K. Kim, J. Chem. Phys. 115, 739 (2001). Anisotropy Quantum Beat in Two-Photon Ionization of Coherently Excited Hyperfine States of Na
Photo of Young Lee

Position:

Research Chemist
Biosystems and Biomaterials Division
Biomaterials Group

Employment History:

2013 - present: Research Chemist, Biomaterials Group, Biosystems and Biomaterials Division

2006 - 2012: Guest Researcher, Biomaterials Group, Polymers Division, NIST

2002 - 2006: Postdoctoral Fellow, University of Texas at Austin

Education:

Ph.D., Physical Chemistry, Seoul National University, (2001)

M.S., Physical Chemistry, Seoul National University, (1996)

B.S., Chemistry, Seoul National University, (1994)

Contact

Phone: (301) 975-6832
Email: youngjong.lee@nist.gov
Fax: (301) 975-4977