Label-Free Chemical Imaging of Live Cells and Tissues Using CARS Microscopy
Phonon Dynamics of Carbon Nanotubes and Graphene
Quantitative Chemical Image Analysis Using Multivariate Analysis Methods
Dr. Lee's current interest is (1) to develop CARS microscopy for a label-free chemical imaging of live cells and tissues and (2) to obtain spatially resolved dynamics of vibrational states or phonons of highly heterogeneous materials, including carbon nanotubes and graphene.
Recently improved sensitivity has demonstrated broadband CARS imaging can obtain Raman chemical images of cells and tissues at least ten times faster than state-of-the-art confocal Raman microscopy. (Biophys. J. (2010)) Dr. Lee is interested in chemical imaging of cells at different differentiation stages and in tracking biological activity occurring in individual cells. This sensitive chemical imaging method will be able to provide 3D physical and chemical structures of other complex materials systems including polymer blends, biofilms, and hydrogels.
Dr. Lee's is also working on a project of vibrational dynamics imaging of nano- and micromaterials using the novel time-resolved CARS technique, which can measure the vibrational dephasing times and population decay times of the same Raman modes at the same sample location with a high spatial resolution at the optical diffraction limit. A recent report (Phys. Rev. B (2010)) demonstrates that phonon dynamics of the G-mode of semiconducting single walled carbon nanotubes in different molecular environments can be measured, suggestive of different influence on the pure dephasing and population decay dynamics. Dr. Lee is also currently studying spatially resolved phonon dynamics in single- and a few-layer graphene.
Publications at NIST
Y. J. Lee, D. Moon, K. B. Migler, M. T. Cicerone, Anal. Chem. 83, 2733 (2011).
Quantitative Image Analysis of Broadband CARS Hyperspectral Images of Polymer Blends.
Y. J. Lee, S. H. Parekh, F. A. Fagan, M. T. Cicerone, Phys. Rev. B in press (2010).
Phonon dephasing and population decay dynamics of the G-band of semiconducting single-wall carbon nanotubes.
S. H. Parekh, Y. J. Lee, K. A. Aamer, M. T. Cicerone, Biophys. J. in press (2010).
Label-free cellular imaging by broadband coherent anti-Stokes Raman scattering microscopy.
K. Chatterjee, S. Lin-Gibson, W. E. Wallace, S. H. Parekh, Y. J. Lee, M. T. Cicerone, M. F. Young, C. G. Simon, Jr., Biomaterials31, 5051 (2010).
The Effect of 3D Hydrogel Scaffold Modulus on Osteoblast Differentiation and Mineralization Revealed by Combinatorial Screening Biomaterials
Y. J. Lee, S. H. Parekh, Y. H. Kim, M. T. Cicerone, Opt. Express 18, 4371 (2010).
Optimized continuum from a photonic crystal fiber for broadband time-resolved coherent anti-Stokes Raman scattering.
Y. Liu, Y. J. Lee, M. T. Cicerone, Opt. Lett. 34, 1363 (2009).
Broadband CARS Spectral Phase Retrieval Using a Time-Domain Kramers-Kronig Transform.
Y. J. Lee, M. T. Cicerone, Opt. Express 17, 123 (2009).
Single-shot interferometric approach to background free broadband coherent anti-Stokes Raman scattering spectroscopy.
Y. Liu, Y. J. Lee, M. T. Cicerone, J. Raman Spectrosc. 40, 726 (2009).
Fast Extraction of Resonant Vibrational Response from CARS Spectra with Arbitrary Nonresonant Background.
Y. J. Lee, M. T. Cicerone, Appl. Phys. Lett. 92, 041108 (2008).
Vibrational Dephasing Time Imaging by Time-Resolved Broadband Coherent Anti-Stokes Raman Scattering Microscopy.
Y. J. Lee, Y. Liu, M. T. Cicerone, Opt. Lett. 32, 3370 (2007).
Characterization of 3-color CARS in a 2-pulse broadband CARS Spectrum.
Publications before NIST
Y. J. Lee, D. Y. Kim, P. F. Barbara, J. Phys. Chem. B110, 9739 (2006).
Effects of Sample Preparation and Excitation Conditions on the Single Molecule Spectroscopy of Conjugated Polymers.
Y. J. Lee, S.-J. Park, A. J. Gesquiere, P. F. Barbara, Appl. Phys. Lett. 87, 051906 (2005).
Probing a Molecular Interface in a Functioning Organic Diode.
Y. J. Lee, D. Y. Kim, J. K. Grey, P. F. Barbara, ChemPhysChem 6, 2404 (2005).
Variable Temperature Single-Molecule Dynamics of MEH–PPV.
J. K. Grey, D. Y. Kim, Y. J. Lee, J. J. Gutierrez, N. Luong, J. P. Ferraris, P. F. Barbara, Angew. Chem. Int. Ed. 44, 6207 (2005).
Single Molecule Studies of Electronic Energy Transfer in a Diblock Conjugated Polymer.
P. F. Barbara, A. J. Gesquiere, S.-J. Park, Y. J. Lee, Acc. Chem. Res. 38, 602 (2005).
Single Molecule Spectroscopy of Conjugated Polymers.
A. J. Gesquiere, Y. J. Lee, J. Yu, P. F. Barbara, J. Phys.Chem. B 109, 12366 (2005).
Single Molecule Modulation Spectroscopy of Conjugated Polymers.
T. Zhang, Y. J. Lee, T. W. Kee, P. F. Barbara, Chem. Phys. Lett. 403, 257 (2005).
The Mechanism of Electron–Cation Geminate Recombination in Liquid Isooctane.
Y. J. Lee, T. Zhang, P. F. Barbara, J. Phys. Chem. B 108, 5175 (2004).
Kinetics of Electron Attachment to Reverse Micelles.
Y. J. Lee, T. W. Kee, T. Zhang, P. F. Barbara, J. Phys. Chem. B 108, 3474 (2004).
Pump–Probe Spectroscopy of the Hydrated Electron in Reverse Micelles.
S. M. Jin, Y. J. Lee, J. Yu, S. K. Kim, Bull. Korean Chem. Soc. 25, 1829 (2004).
Development of Femtosecond Stimulated Raman Spectroscopy: Stimulated Raman Gain via Elimination of Cross Phase Modulation.
Y. J. Lee, N. W. Song, S. K. Kim, J. Phys.Chem. A 106, 5582 (2002).
Photoluminescence of C60 and Its Photofragments in the Gas Phase.
Y. J. Lee, S. H. Cho, N. J. Kim, S. K. Kim, J. Chem. Phys. 115, 739 (2001).
Anisotropy Quantum Beat in Two-Photon Ionization of Coherently Excited Hyperfine States of Na.
N. W. Song, Y. J. Lee, S. K. Kim, D. Kim, Phys. Rev. Lett. 73, 2634 (1994).
Comment on "Nanosecond-Gated Detection of Room-Temperature Fluorescence of C60 in Solution."
Biosystems and Biomaterials Division
2006 - present: Guest Researcher, Biomaterials Group, Polymers Division, NIST
2002 - 2006: Postdoctoral Fellow, University of Texas at Austin
Dr. Lee joined the Biomaterials Group of the Polymers Division at NIST in 2006 to work on the Coherent Anti-Stokes Raman Scattering (CARS) imaging project for living tissue and cellular chemical imaging and for phonon dynamics imaging of nanomaterials. His major physical chemistry research background is based on time-resolved spectroscopy on the photo-induced dynamics of energy transfer and charge transfer in various physical and chemical systems.
Ph.D., Physical Chemistry, Seoul National University, (2001)
M.S., Physical Chemistry, Seoul National University, (1996)
B.S., Chemistry, Seoul National University, (1994)
Phone: (301) 975-6832