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Publication Citation: Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells

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Author(s): Subhadip Bodhak; Sheng Lin-Gibson; Carl G. Simon Jr; Luis Fernandez de Castro Diaz; Pamela G. Robey; Sergei A. Kuznetsov; Tina Kilts; Marian F. Young;
Title: Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells
Published: April 19, 2014
Abstract: The use of in vivo transplantation assays under defined experimental conditions has become a valuable standard for evaluating osteogenic potential of different stem cell populations and is the subject of ongoing pre-clinical reconstructive therapy research. Therefore, development of a cost effective in vivo transplantation assay technique offering low volume sampling and rapid analysis in a high-throughput manner is of great interest due to the escalating health care costs. Herein, we have successfully validated an in vivo combinatorial transplant model by developing a miniature cassette transplant with multiple slots for screening osteogenesis of various human bone marrow stromal cells (hBMSCs) and bone grafting formulations (e.g., HA/TCP powder, Gelfoam) in subcutaneous space of immunodeficient mice. Our results confirmed that microwell-type combinatorial cassette transplant model can be used in in vivo cell based transplantation assay applications to efficiently, systematically and rapidly test as many as hBMSCs/vehicles combinations for maximizing osteogenesis.
Proceedings: Society for Biomaterials 2014 Annual Meeting & Exposition
Volume: 1
Issue: 1
Pages: pp. 1 - 1
Location: Denver, CO
Dates: April 16-19, 2014
Keywords: Combinatorial In Vivo Screening; Osteogenesis; Human Bone Marrow Stromal Cells; Mouse subcutaneous surgery, Histology
Research Areas: Biomaterials, Tissue Engineering