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|Author(s):||Subhadip Bodhak; Sheng Lin-Gibson; Carl G. Simon Jr.; Luis Fernandez de Castro Diaz; Pamela G. Robey; Sergei A. Kuznetsov; Tina Kilts; Marian F. Young;|
|Title:||Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells|
|Published:||April 19, 2014|
|Abstract:||The use of in vivo transplantation assays under defined experimental conditions has become a valuable standard for evaluating osteogenic potential of different stem cell populations and is the subject of ongoing pre-clinical reconstructive therapy research. Therefore, development of a cost effective in vivo transplantation assay technique offering low volume sampling and rapid analysis in a high-throughput manner is of great interest due to the escalating health care costs. Herein, we have successfully validated an in vivo combinatorial transplant model by developing a miniature cassette transplant with multiple slots for screening osteogenesis of various human bone marrow stromal cells (hBMSCs) and bone grafting formulations (e.g., HA/TCP powder, Gelfoam) in subcutaneous space of immunodeficient mice. Our results confirmed that microwell-type combinatorial cassette transplant model can be used in in vivo cell based transplantation assay applications to efficiently, systematically and rapidly test as many as hBMSCs/vehicles combinations for maximizing osteogenesis.|
|Proceedings:||Society for Biomaterials 2014 Annual Meeting & Exposition|
|Pages:||pp. 1 - 1|
|Dates:||April 16-19, 2014|
|Keywords:||Combinatorial In Vivo Screening, Osteogenesis, Human Bone Marrow Stromal Cells, Mouse subcutaneous surgery, Histology|
|Research Areas:||Biomaterials, Tissue Engineering|